The global pandemic due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to ∼6.9 million deaths and affected ∼767 million people globally. The approved vaccines have contributed significantly in reducing disease severity and ...
The biopharmaceutical industry is currently being presented with opportunities to improve research and business efficiency via automation and the integration of various systems. In the examples discussed, industrial high-throughput screening systems are ...
Functional knowledge of individual genes encoding components of the cell signaling, metabolic and regulatory pathways is crucial to our understanding of physiology and pathophysiology. A central challenge in functional genomics is the creation of a ...
The effect of a drug is related to the interaction of the numerous gene products that participate in a drug pathway. The clinical importance of genetic variability on the overall outcome of most drug pathways remains to be determined. As a result, there ...
Cell-based microarrays were first described by Ziauddin and Sabatini in 2001 as a novel method for performing high-throughput screens of gene function. In this study, expression vectors containing the open reading frame of human genes were printed onto ...
RNAi screening in mammalian cells has become a valuable method to identify and describe genetic relationships in both basic biology and disease mechanisms. Multiple efforts are underway to standardize how RNAi screening data are reported, including ...
In the last 13 years, three gene therapy trials for the treatment of α-1 antitrypsin deficiency have been conducted. The first trial delivered plasmid encoding the α-1 antitrypsin cDNA to the nasal epithelium using cationic liposomes. The last two trials ...
Alteration in epigenetic regulation of gene expression is a frequent event in human cancer. CpG island hypermethylation and downregulation is observed for many genes involved in a diverse range of functions and pathways that become deregulated in cancer. ...
Evaluation of: Sonoke S, Ueda T, Fujiwara K et al.: Tumor regression in mice by delivery of Bcl2 small interfering RNA with pegylated cationic liposomes. Cancer Res. 68, 8843–8851 (2008). The induction of RNAi, which represents a powerful, naturally ...
Resistance including multidrug resistance to chemotherapy is a common clinical problem in patients suffering from cancer. Multidrug resistance is often mediated by overexpression of transmembrane xenobiotic transport molecules belonging to the superfamily ...
Lentiviral-based genetic therapies offer a valuable addition to the current anti-HIV arsenal and allow for a rational directed approach to evolve HIV-1 to a less pathogenic state. Many lentiviral vector systems have been described that can be either ...
Evaluation of: Medarova Z, Pham W, Farrar C, Petkova V, Moore A: In vivo imaging of siRNA delivery and silencing in tumors. Nat. Med. 13(3), 372–377 (2007)[1]. A recent paper on the fabrication and biological evaluation of a multifunctional nanoparticle ...
Nanoparticles (NPs) and nanosized objects are being incorporated rapidly into clinical medicine and particularly into the field of medical oncology, including breast cancer. A number of novel methods for breast cancer diagnosis and treatment, which are ...
Controlling HIV infection continues to be a major clinical and scientific challenge. Despite the therapeutic benefits associated with HAART, the need for novel treatment approaches to combat HIV-1 remains. Effective inhibition of HIV-1 infection has been ...
Alterations in epigenetic processes probably underlie most human malignancies. Novel genome-wide techniques, such as chromatin immunoprecipitation and high-throughput sequencing, have become state-of-the-art methods to map the epigenomic landscape of ...
Inclisiran is a siRNA inhibiting hepatic PCSK9 synthesis. As a first-in-class therapy, inclisiran has been assessed within the ORION trial program for its low-density lipoprotein cholesterol (LDL-C) lowering efficacy and clinical safety. Phase II and III ...
Aim: To predict siRNAs as a therapeutic intervention for highly infectious new variants of SARS-CoV-2. Methods: Conserved coding sequence regions of 11 SARS-CoV-2 proteins were used to construct siRNAs through sampling of metadata comprising 214,256 ...
The recent development of RNAi-based techniques for protein knockdown in mammalian cells has allowed for unprecedented flexibility in the study of protein function. Currently, large siRNA libraries are available that allow the knockdown of all proteins ...
Chronic HBV infection remains an important public health problem and currently licensed therapies rarely prevent complications of viral persistence. Silencing HBV gene expression using gene therapy, particularly with exogenous activators of RNAi, holds ...