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Concentration and integrity indexes of urine cell-free DNA as promising biomarkers for early lung cancer diagnosis

    Sai Ren

    Department of Laboratory Medicine, Daping Hospital, Army Medical University, Chongqing 400042, PR China

    ,
    Xiaodong Ren

    Department of Laboratory Medicine, Daping Hospital, Army Medical University, Chongqing 400042, PR China

    ,
    Haiqin Guo

    Department of Pulmonology, Southwest Hospital, Army Medical University, Chongqing 400038, PR China

    ,
    Lan Liang

    Department of Pulmonology, Southwest Hospital, Army Medical University, Chongqing 400038, PR China

    ,
    Kun Wei

    Department of Laboratory Medicine, Daping Hospital, Army Medical University, Chongqing 400042, PR China

    ,
    Lifang Guo

    Department of Laboratory Medicine, Daping Hospital, Army Medical University, Chongqing 400042, PR China

    ,
    Xuemei Qu

    Department of Laboratory Medicine, Daping Hospital, Army Medical University, Chongqing 400042, PR China

    ,
    Xiaotian Dai

    **Author for correspondence: Tel.: +86 23 6876 5180;

    E-mail Address: daixt1973@163.com

    Department of Pulmonology, Southwest Hospital, Army Medical University, Chongqing 400038, PR China

    &
    Qing Huang

    *Author for correspondence: Tel.: +86 23 6875 7601;

    E-mail Address: dr.q.huang@gmail.com

    Department of Laboratory Medicine, Daping Hospital, Army Medical University, Chongqing 400042, PR China

    Published Online:https://doi.org/10.2217/pme-2020-0019

    Aim: To explore the role of urine cell-free DNA (ucfDNA) concentration and integrity indexes as potential biomarkers for lung cancer diagnosis. Materials & methods: Quantitative real-time PCR targeting Arthrobacter luteus (ALU) repeats at three size fragments (ALU-60, 115 and 247 bp) was performed in 55 lung cancer patients and 35 healthy individuals. Results: ucfDNA concentration and integrity indexes were significantly higher in lung cancer patients than in healthy controls. The area under the receiver operating characteristic curve for differentiating patients with stage I/II from healthy controls by ALU fragments concentration were 0.856, 0.909 and 0.932, respectively. In addition, the ucfDNA integrity indexes in patients with lymph node metastasis were significantly higher than in patients with non-metastatic. Conclusion: ucfDNA concentration and integrity indexes could serve as promising biomarkers for lung cancer diagnosis.

    Papers of special note have been highlighted as: • of interest; •• of considerable interest

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