Detection of colonization capacity of probiotic Bifidobacterium breve CCFM1025 in the human gut
Abstract
Aim: To detect the gut colonization capacity of Bifidobacterium breve CCFM1025 with clinical antidepressant-like effects. Materials & methods: A unique gene sequence of B. breve CCFM1025 was discovered based on the genome analysis of 104 B. breve strains and a strain-specific primer (1025T5) was designed. In vitro and in vivo samples were used to validate the specificity and quantitative capability of this primer in the PCR system. Results: Quantitative PCR using strain-specific primers enabled absolute quantification of CCFM1025 in fecal samples within 104–1010 cells/g (R2 >0.99). CCFM1025 remained highly detectable in volunteer feces 14 days after cessation of administration, demonstrating its favorable colonization characteristics. Conclusion: CCFM1025 can colonize the healthy human gut.
Tweetable abstract
Investigators developed an assay based on a specific gene to quantify Bifidobacterium in the human gut. Autochthonous B. breve in the human gut exhibited stronger gut colonization capacity than some exogenous commercial probiotics.
Papers of special note have been highlighted as: • of interest; •• of considerable interest
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