Research Article

Department of Plastic Surgery, The Third Xiangya Hospital of Central South University, Changsha 410013, Hunan, PR China

Department of Cosmetic & Plastic Surgery, The First People’s Hospital of Chenzhou, Chenzhou 423000, Hunan, PR China

^‡Authors contributed equally

Department of Plastic Surgery, The Third Xiangya Hospital of Central South University, Changsha 410013, Hunan, PR China

Center for Medical Genetics & Hunan Key Laboratory of Medical Genetics, School of Life Sciences, Central South University, Changsha, Hunan, PR China

^‡Authors contributed equally

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Hongbo Xu^‡

Department of General Surgery, The Third Xiangya Hospital of Central South University, Changsha 410013, Hunan, PR China

^‡Authors contributed equally

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Qiangru Huang

Department of Plastic Surgery, The Third Xiangya Hospital of Central South University, Changsha 410013, Hunan, PR China

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Zi Wen

Department of Plastic Surgery, The Third Xiangya Hospital of Central South University, Changsha 410013, Hunan, PR China

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Yawei Liu

Department of Plastic Surgery, The Third Xiangya Hospital of Central South University, Changsha 410013, Hunan, PR China

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Cheng Peng

*Author for correspondence:

E-mail Address: pcheng83@sina.com

Department of Plastic Surgery, The Third Xiangya Hospital of Central South University, Changsha 410013, Hunan, PR China

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Published Online:3 Dec 2019https://doi.org/10.2217/nnm-2018-0487

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Abstract

Aim: Diabetic wound healing is seriously interrupted, and administration of KGF for wound treatment is restricted by its inherent instability. We aim to develop an ideal way toward KGF stabilization, thus improving diabetic wound healing. Materials & methods: We conjugated KGF with gold nanoparticles (GNPs) and determined the stability and binding affinity. Biological effects of conjugates (KGF-GNPs) were evaluated in vitro and in an animal model. Results: KGF-GNPs revealed high stability under hostile circumstances because of the preserved secondary structure and possessed elevated binding affinity to KGF receptor. Moreover, application of KGF-GNPs contributed to accelerated wound recovery in diabetic rats, including re-epithelialization and contraction. Conclusion: KGF-GNPs were promising for future clinical application for diabetic wound therapy.

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Papers of special note have been highlighted as: • of interest; •• of considerable interest

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Vol. 14, No. 22

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History

Received 14 December 2018

Accepted 3 September 2019

Published online 3 December 2019

Published in print November 2019

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Keywords

Author contributions

C Peng designed and supervised the study. Q Tang, S Li, Z Wen and Y Liu performed experiments. Q Tang, S Li, H Xu and Q Huang contributed to data acquisition and analysis. S Li, Q Tang and H Xu wrote the first draft of the manuscript. All authors contributed in revising the manuscript and approved the final manuscript.

Financial & competing interests disclosure

This work was financially supported by National Natural Science Foundation of China (grant number 81301636), Health Commission of Hunan Province (grant number 20180961). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

All animal experiments were conducted in accordance with the guidelines and the ethical standards of Animal Care and Use Committee of Central South University.

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Improved stability of KGF by conjugation with gold nanoparticles for diabetic wound therapy

Abstract

References