Research Article

Key Laboratory of Major Diseases in Children & National Key Discipline of Pediatrics, Ministry of Education, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing, China

^‡Authors contributed equally

Junhong Ai^‡

^‡Authors contributed equally

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Yali Liu

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Chunyan Liu

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Zhengde Xie

*Author for correspondence:

E-mail Address: xiezhengde@bch.com.cn

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Published Online:18 Nov 2014https://doi.org/10.2217/fvl.14.71

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Abstract

ABSTRACT

The viral IL-10 (vIL-10) gene of Epstein–Barr virus (EBV) exhibits high homology with human IL-10 gene. In order to characterize vIL-10 gene in EBV-infected children in China, DNA sequencing of this gene was performed in 249 cases of EBV-associated infectious mononucleosis, 46 EBV-associated hemophagocytic lymphohistiocytosis and six chronic active EBV infection. Eight silent and seven nonsilent mutations were identified, of which silent mutation c9980a was prominently presented (75.7%, 228/301) in the studied cases. Two subtypes of the vIL-10 gene, including pattern B95–8 and pattern SPM (mutation in the signal peptide), were identified. Pattern B95-8 was the dominant subtype in infectious mononucleosis, EBV-hemophagocytic lymphohistiocytosis and chronic active EBV infection. These results indicated that the vIL-10 gene exists as a highly conservative form in EBV-associated infection in Chinese children.

Keywords:

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Vol. 9, No. 10

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Published online 18 November 2014

Published in print October 2014

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Acknowledgements

The authors thank the staff of virology laboratory in Beijing Children's Hospital for collecting the samples used in these studies.

Author contributions

W Lu and J Ai carried out most of the experiments and wrote the manuscript. Y Liu and C Liu helped with the experiments. Z Xie designed the experiments and revised the manuscript. All of the authors read and approved the final version of this manuscript.

Financial & competing interests disclosure

The authors express their thanks to the research project sponsored by the Natural Science Foundation of China (NSFC; no. 81241066). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct

The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.

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Characteristics of viral IL-10 gene in Epstein–Barr virus infection in children in China

Abstract

ABSTRACT

References