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Characterization of a monoclonal antibody that binds to both gp120 and gp41

    Hao Zheng

    Department of Radiology, Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, PR China

    ,
    Jiansong Tang

    China Bioengineering Technology Group Limited, Unit 209, Building 16W, Hong Kong Science Park, Shatin, NT, HK 999077, PR China

    ,
    Shiqiang Lu

    Department of Antibody Engineering, Simcere Pharmaceutical Group, Nanjing, PR China

    ,
    Qian Qian

    Soochow University Laboratory of Cancer Molecular Genetics, Medical College of Soochow University, Suzhou 215123, PR China

    ,
    Wan Liu

    Department of Medicine, Faculty of Medicine, the University of Hong Kong, Pokfulam, Hong Kong, PR China

    ,
    Zheng Yang

    Department of Tuberculosis Prevention, Shenzhen Center for Chronic Disease Control, Shenzhen, PR China

    ,
    Bing Liu

    Department of Respiratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, PR China

    ,
    Long Long

    School of Dental Medicine, University of Colorado Denver, Denver, CO, USA

    ,
    Xiaomei Ding

    China Bioengineering Technology Group Limited, Unit 209, Building 16W, Hong Kong Science Park, Shatin, NT, HK 999077, PR China

    ,
    Pinya Lin

    China Bioengineering Technology Group Limited, Unit 209, Building 16W, Hong Kong Science Park, Shatin, NT, HK 999077, PR China

    ,
    Johnny Pun

    China Bioengineering Technology Group Limited, Unit 209, Building 16W, Hong Kong Science Park, Shatin, NT, HK 999077, PR China

    ,
    Kiana Wong

    China Bioengineering Technology Group Limited, Unit 209, Building 16W, Hong Kong Science Park, Shatin, NT, HK 999077, PR China

    ,
    Zhao Yin

    China Bioengineering Technology Group Limited, Unit 209, Building 16W, Hong Kong Science Park, Shatin, NT, HK 999077, PR China

    ,
    Tattung Wong

    Hong Kong Institute of Precision Health Management Limited, United Centre, Hong Kong, PR China

    ,
    Wing TY Loo

    Precision Health Research Center Company Limited, Shatin, Hong Kong, PR China

    ,
    Ke Zhang

    The Key and Characteristic Laboratory of Modern Pathogen Biology, Guizhou Medical University, Department of Education of Guizhou Province, Guiyang, PR China

    Department of Parasitology, Basic Medical College, Guizhou Medical University, Guiyang, PR China

    ,
    Hui Huang

    *Author for correspondence:

    E-mail Address: huanghnj@qq.com

    China Bioengineering Technology Group Limited, Unit 209, Building 16W, Hong Kong Science Park, Shatin, NT, HK 999077, PR China

    Nanjing University of Information Science & Technology, Nanjing, Jiangsu, PR China

    Authors contributed equally

    Search for more papers by this author

    &
    Jianguo Liang

    **Author for correspondence:

    E-mail Address: jayliang@cbtgene.hk

    China Bioengineering Technology Group Limited, Unit 209, Building 16W, Hong Kong Science Park, Shatin, NT, HK 999077, PR China

    Authors contributed equally

    Search for more papers by this author

    Published Online:https://doi.org/10.2217/fvl-2018-0097

    Aim: AIDS has become a global pandemic. Characterization of broadly HIV-1-neutralizing antibodies (bnAbs) may facilitate the vaccine design. Methods & materials: Recombinant antibody library construction provides a resourceful way of monoclonal antibody screening and isolation against HIV-1. Results: In this study, we screened a novel human monoclonal antibody, named 2B8, which can bind both the gp120 and gp41 subunits of the HIV-1 envelope glycoprotein (Env). 2B8 did not bind to the CD4 binding site mutant, gp120 D368R, suggesting that the 2B8 epitope is conformational and overlaps the CD4 binding site on gp120. 2B8 neutralized 50% of the HIV-1 cell line-based pseudo virus isolates tested. Conclusion: The further study of its novel epitope may reveal the new mechanism of neutralization and assist the design of vaccine immunogens against HIV-1.

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