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miRNA-767 and its binding site polymorphism in the mTOR gene act as potential biomarkers for female reproductive cancers

    Rabia Shafique

    Cancer Genetics & Epigenetics Research Group, Department of Biosciences, COMSATS University, Park Road Islamabad, Pakistan

    ,
    Ishrat Mahjabeen

    Cancer Genetics & Epigenetics Research Group, Department of Biosciences, COMSATS University, Park Road Islamabad, Pakistan

    ,
    Kashaf Bibi

    Cancer Genetics & Epigenetics Research Group, Department of Biosciences, COMSATS University, Park Road Islamabad, Pakistan

    ,
    Farah Kalsoom

    Department of Pathology, Sir Ganga Ram Hospital, Lahore, Pakistan

    ,
    Muhammad Rizwan

    Cancer Genetics & Epigenetics Research Group, Department of Biosciences, COMSATS University, Park Road Islamabad, Pakistan

    ,
    Nida Sarosh Ashraf

    Cancer Genetics & Epigenetics Research Group, Department of Biosciences, COMSATS University, Park Road Islamabad, Pakistan

    ,
    Azhar Mehmood

    Cancer Genetics & Epigenetics Research Group, Department of Biosciences, COMSATS University, Park Road Islamabad, Pakistan

    ,
    Maria Fazal Ul Haq

    Cancer Genetics & Epigenetics Research Group, Department of Biosciences, COMSATS University, Park Road Islamabad, Pakistan

    ,
    Sumaira Fida Abbasi

    Cancer Genetics & Epigenetics Research Group, Department of Biosciences, COMSATS University, Park Road Islamabad, Pakistan

    ,
    Nadia Saeed

    Cancer Genetics & Epigenetics Research Group, Department of Biosciences, COMSATS University, Park Road Islamabad, Pakistan

    &
    Mahmood Akhtar Kayani

    *Author for correspondence:

    E-mail Address: mkayani@comsats.edu.pk

    Cancer Genetics & Epigenetics Research Group, Department of Biosciences, COMSATS University, Park Road Islamabad, Pakistan

    Published Online:https://doi.org/10.2217/fon-2022-1055

    Aims: The present study aimed to understand the relationship between the mTOR gene SNP (rs2536) and reproductive cancer risk. The expression level of miRNA-767 was also assessed. Methods: 700 tumor samples (300 breast, 200 ovarian and 200 cervical cancers), along with adjacent uninvolved control tissue, were used. rs2536 was screened using Tetra-ARMS PCR and expression level of miRNA-767 was assessed using quantitative PCR. Results: The frequency of the homozygous mutant genotype of rs2536 was observed significantly higher in breast (p < 0.04), ovarian (p < 0.005) and cervical (p < 0.003) cancers. Significant downregulation of miRNA-767 was observed in tumors compared with controls. Conclusion: The present study demonstrates that increased mutant frequency of rs2536 and deregulation of miRNA-767 are associated with increased reproductive cancer risk.

    Papers of special note have been highlighted as: • of interest; •• of considerable interest

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