Clinical implementation of a procedure to prepare bone marrow cells from cadaveric vertebral bodies
Abstract
Aims: Vertebral bone marrow is a rich and easily accessible source of hematopoietic and mesenchymal stem cells that has been used to promote chimerism and transplantation tolerance in connection with cadaveric organ transplantation. The purpose of this study is to provide a detailed account of the procedure used to prepare the first five vertebral bone marrow products for infusion in conjunction with the first hand/hand–forelimb transplants performed at the University of Pittsburgh (PA, USA). Materials & methods: The cell separation and release testing were performed at the University of Pittsburgh Cancer Institute’s Hematopoietic Stem Cell Laboratory, a Good Manufacturing Practice-compliant facility accredited for clinical cell processing by the Foundation for Accreditation of Cellular Therapy (FACT) and for clinical flow cytometry by the College of American Pathologists (CAP).
Bibliography
- 1 Fontes P, Rao AS, Carroll P et al. Bone marrow augmentation of donor–cell chimerism in kidney, liver, heart, and pancreas islet transplantation. Lancet344,151–155 (1994).
- 2 Rao AS, Fontes P, Zeevi A et al. Combined bone marrow and whole organ transplantation from the same donor. Transplant. Proc.26,3377–3378 (1994).
- 3 Pham SM, Keenan RJ, Rao AS et al. Perioperative donor bone marrow infusion augments chimerism in heart and lung transplant recipients. Ann. Thorac. Surg.60,1015–1020 (1995).
- 4 Rao AS, Phil D, Fontes P et al. An attempt to induce tolerance with infusion of donor bone marrow in organ allograft recipients. Adv. Exp. Med. Biol.417,269–274 (1997).
- 5 Millan MT, Shizuru JA, Hoffmann P et al. Mixed chimerism and immunosuppressive drug withdrawal after HLA-mismatched kidney and hematopoietic progenitor transplantation. Transplantation73,1386–1391 (2002).
- 6 Azari KK, Imbriglia JE, Goitz RJ et al. Technical aspects of the recipient operation in hand transplantation. J. Reconstr. Microsurg. doi: 10.1055/s-0031-1285820 (2011) (Epub ahead of print).
- 7 Gorantla VS, Schneeberger S, Moore LR et al. Development and validation of a procedure to isolate viable bone marrow cells from the vertebrae of cadaveric organ donors for composite organ grafting. Cytotherapy DOI: 10.3109/14653249.2011.605350 (2011) (Epub ahead of print).
- 8 Clinical and Laboratory Standards Institute.Laboratory Documents: Development and Control; Approved Guideline (Fifth Edition). Clinical and Laboratory Standards Institute, Wayne, PA, USA, 1–81 (2006).
- 9 Sutherland DR, Anderson L, Keeney M, Nayar R, Chin-Yee I. The ISHAGE guidelines for CD34+ cell determination by flow cytometry. International Society of Hematotherapy and Graft Engineering. J. Hematother.5,213–226 (1996).
- 10 Donnenberg AD, Koch EK, Griffin DL et al. Viability of cryopreserved BM progenitor cells stored for more than a decade. Cytotherapy4,157–163 (2002).
- 11 Mather JP, Roberts PE. Acridine orange–ethidium bromide viability determination. In: Introduction to Cell and Tissue Culture: Theory and Technique. Plenum Press, New York, NY, USA, 74 (1998).
- 12 Donnenberg AD. T-cell depletion and allograft engineering. In: Hematopoietic Stem Cell Therapy. Ball ED, Lister J, Law P (Eds). Churchill Livingstone, Philadelphia, PA, USA 335–344 (2000).
- 13 Sahota A, Gao S, Hayes J, Jindal RM. Microchimerism and rejection: a meta-analysis. Clin. Transplant.14,345–350 (2000).
- 14 Sharp TG, Sachs DH, Matthews JG, Maples J, Woody JN, Rosenberg SA. Harvest of human bone marrow directly from bone. J. Immunol. Methods69,187–195 (1984).
- 15 Rybka WB, Fontes PA, Rao AS et al. Hematopoietic progenitor cell content of vertebral body marrow used for combined solid organ and bone marrow transplantation. Transplantation59,871–874 (1995).
- 16 Moore S. Pancreatic DNase. In: The Enzymes (Volume 14). Boyer PD (Ed.). Academic Press, NY, USA, 281–296 (1981).
- 17 Windrum P, Morris TCM, Drake MB, Niederwieser D, Ruutu T. Variation in dimethyl sulfoxide use in stem cell transplantation: a survey of EBMT centres. Bone Marrow Transplant.36,601–603 (2005).