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Regenerative Medicine

Clinical implementation of a procedure to prepare bone marrow cells from cadaveric vertebral bodies

    Albert D Donnenberg

    † Author for correspondence

    Department of Medicine, University of Pittsburgh School of Medicine, PA, USA.

    ,
    Vijay S Gorantla

    McGowan Institute for Regenerative Medicine, PA, USA

    Department of Surgery, University of Pittsburgh School of Medicine, PA, USA

    ,
    Stefan Schneeberger

    Department of Plastic & Reconstructive Surgery, Johns Hopkins University School of Medicine, MD, USA

    Department of General, Thoracic & Transplant Surgery, Innsbruck Medical University, Innsbruck, Austria

    ,
    Linda R Moore

    Hillman Cancer Center, University of Pittsburgh Medical Center, 5117 Centre Avenue, Suite 2.42 Research Pavilion, Pittsburgh, PA 15213, USA

    ,
    Gerald Brandacher

    Department of Plastic & Reconstructive Surgery, Johns Hopkins University School of Medicine, MD, USA

    ,
    Heather M Stanczak

    Hillman Cancer Center, University of Pittsburgh Medical Center, 5117 Centre Avenue, Suite 2.42 Research Pavilion, Pittsburgh, PA 15213, USA

    ,
    Eileen K Koch

    Hillman Cancer Center, University of Pittsburgh Medical Center, 5117 Centre Avenue, Suite 2.42 Research Pavilion, Pittsburgh, PA 15213, USA

    &
    WP Andrew Lee

    Department of Plastic & Reconstructive Surgery, Johns Hopkins University School of Medicine, MD, USA

    Published Online:https://doi.org/10.2217/rme.11.89

    Aims: Vertebral bone marrow is a rich and easily accessible source of hematopoietic and mesenchymal stem cells that has been used to promote chimerism and transplantation tolerance in connection with cadaveric organ transplantation. The purpose of this study is to provide a detailed account of the procedure used to prepare the first five vertebral bone marrow products for infusion in conjunction with the first hand/hand–forelimb transplants performed at the University of Pittsburgh (PA, USA). Materials & methods: The cell separation and release testing were performed at the University of Pittsburgh Cancer Institute’s Hematopoietic Stem Cell Laboratory, a Good Manufacturing Practice-compliant facility accredited for clinical cell processing by the Foundation for Accreditation of Cellular Therapy (FACT) and for clinical flow cytometry by the College of American Pathologists (CAP).

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