Research Article

Astragaloside-IV induces the differentiation of bone marrow mesenchymal stem cells into osteoblasts through NMUR2-mediated Wnt/β-catenin pathway

Emergency Trauma Center, Henan Province Hospital of TCM, Jinshui District, Zhengzhou City, Henan Province, 450002, China

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Qiuxia Lv

Department of Anorectal, Henan Province Hospital of TCM, Jinshui District, Zhengzhou City, Henan Province, 450002, China

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Zhihui Huang

*Author for correspondence:

E-mail Address: hzhihui_huangzhh@163.com

https://orcid.org/0000-0001-9071-7045

Emergency Trauma Center, Henan Province Hospital of TCM, Jinshui District, Zhengzhou City, Henan Province, 450002, China

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Yang Li

Emergency Trauma Center, Henan Province Hospital of TCM, Jinshui District, Zhengzhou City, Henan Province, 450002, China

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Published Online:31 May 2023https://doi.org/10.2217/rme-2022-0184

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Abstract

Aim: Given that astragaloside-IV (As-IV) has the ability to promote osteogenic differentiation, its mechanism is worthy of exploration. Methods: The effect of As-IV on rat tibial defects was examined by histopathological staining and MiR-CT scan. The alkaline phosphatase (ALP) content, osteogenic differentiation-related gene expressions, and mineralized nodule formation in bone marrow mesenchymal stem cells (BMSCs) were detected. Results: As-IV repaired tibial defects of rats. As-IV or neuromedin receptor 2 (NMUR2) overexpression elevated ALP content, mineralized nodules, osteogenic differentiation-related genes, β-catenin and NMUR2 levels, the effects of which were reversed by NMUR2 silencing or Wnt/β-catenin pathway inhibitors. Conclusion: As-IV regulates the Wnt/β-catenin pathway through NMUR2 to promote the repair of tibial defects in rats and the differentiation of BMSCs into osteoblasts.

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Vol. 18, No. 6

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History

Received 1 November 2022

Accepted 26 April 2023

Published online 31 May 2023

Published in print June 2023

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Keywords

Author contributions

Substantial contributions to conception and design: Yujing Cao. Data acquisition, data analysis and interpretation: Qiuxia Lv, Zhihui Huang, Yang Li. Drafting the article or critically revising it for important intellectual content: Yujing Cao. Final approval of the version to be published: Yujing Cao, Qiuxia Lv, Zhihui Huang, Yang Li. Agreement to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of the work are appropriately investigated and resolved: Yujing Cao, Qiuxia Lv, Zhihui Huang, Yang Li.

Financial & competing interests disclosure

This work was supported by Henan Province Top Talent Training Program of Chinese Medicine (2019). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations.

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