Research Article

miR-125b-5p alleviates the damage of myocardial infarction by inhibiting the NFAT2 to reduce F2RL2 expression

Academy of Medical Engineering & Translational Medicine, Tianjin University, Tianjin, 300072, China

ICU, Department of Cardiac Surgery, Tianjin Chest Hospital, Tianjin, 300222, China

‡These authors contributed equally to this work

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Jie Geng‡

CICU, Tianjin Chest Hospital, Tianjin, 300222, China

‡These authors contributed equally to this work

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Yunpeng Bai

Department of Cardiac Surgery, Tianjin Chest Hospital, Tianjin, 300222, China

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Yujuan Qi

ICU, Department of Cardiac Surgery, Tianjin Chest Hospital, Tianjin, 300222, China

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Chao Chang

ICU, Department of Cardiac Surgery, Tianjin Chest Hospital, Tianjin, 300222, China

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Yan Jiao

ICU, Department of Cardiac Surgery, Tianjin Chest Hospital, Tianjin, 300222, China

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Zhigang Guo

*Author for correspondence:

E-mail Address: guozhigang_gzg@163.com

https://orcid.org/0000-0001-9555-2593

Department of Cardiac Surgery, Tianjin Chest Hospital, Tianjin, 300222, China

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Published Online:21 Jun 2023https://doi.org/10.2217/rme-2022-0150

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Abstract

Aim: To explore the effect of miR-125b-5p/nuclear factor of activated T cells 1 (NFAT2)/F2RL2 on myocardial infarction (MI). Method: After establishment of MI mouse model and oxygen glucose deprivation (OGD)-induced cell model, the effects of NFAT2 on the process of MI were observed, the effects of miR-125b-5p/NFAT2/F2RL2 on the cell viability, apoptosis, and inflammatory factors levels were determined. Result:NFAT2 silencing relieved MI and inhibited the inflammation in MI model mice. In OGD-induced human coronary artery endothelial cells and human cardiac microvascular endothelial cells, miR-125b-5p enhanced cell viability, yet repressed cell apoptosis and inflammatory factors and NFAT2 levels. NFAT2 overexpression reversed the effects of miR-125b-5p, while F2RL2 silencing offset the effects of NFAT2 overexpression. Conclusion: MiR-125b-5p alleviates MI injury by inhibiting NFAT2 level to reduce F2RL2 expression.

Plain language summary

This research proves that miR-125b-5p reduces the level of F2RL2 by preventing the activation of NFAT2 pathway, thereby reducing cardiogenic vascular endothelial cell damage and inflammation (heat, swelling and redness). This may provide a new treatment for heart attacks.

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Keywords:

Papers of special note have been highlighted as: • of interest

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Vol. 18, No. 7

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History

Received 25 August 2022

Accepted 15 May 2023

Published online 21 June 2023

Published in print July 2023

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Supplementary data

To view the supplementary data that accompany this paper please visit the journal website at: www.futuremedicine.com/doi/suppl/10.2217/rme-2022-0150

Author contributions

Substantial contributions to conception and design: Z Wu, J Geng. Data acquisition, data analysis and interpretation: Y Bai, Y Qi, C Chang, Y Jiao, Z Guo. Drafting the article or critically revising it for important intellectual content: Z Wu, J Geng. Final approval of the version to be published: All authors. Agreement to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of the work are appropriately investigated and resolved: All authors.

Financial & competing interests disclosure

This work was supported by the Tianjin Health Science and Technology Project [ZC20147]. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations.

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