Research Article

Tissue-engineered bones with adipose-derived stem cells – composite polymer for repair of bone defects

Department of Hand & Reconstructive Surgery, Plastic & Reconstructive Surgery Center, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou, Zhejiang, 310014, China

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Jianlong Wu

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Hongrui Lu

*Author for correspondence: Tel.: +86 0571 8589 3485;

E-mail Address: guoenqi@hmc.edu.cn

https://orcid.org/0000-0002-4619-0693

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Liang Wang

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Qiang Chen

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Published Online:15 Jun 2022https://doi.org/10.2217/rme-2022-0044

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Abstract

Background: Development of alternative bone tissue graft materials based on tissue engineering technology has gradually become a research focus. Engineered bone composed of biodegradable, biosafe and bioactive materials is attractive, but also challenging. Materials & methods: An adipose-derived stem cell/poly(L-glutamic acid)/chitosan composite scaffold was further developed for construction of biodegradable and bone-promoting tissue-engineered bone. A series of composite scaffold materials with different physical properties such as structure, pore size, porosity and pore diameter was developed. Results: The composite scaffold showed good biodegradability and water absorption, and exhibited an excellent ability to promote bone differentiation. Conclusion: This type of biodegradable scaffold is expected to be applied to the field of bone repair or bone tissue engineering.

Plain language summary

In recent years, the application of bone graft materials in bone defect repair has become a research hotspot. Engineered bone composed of biodegradable, biosafe and bioactive materials is attractive but also challenging. A composite scaffold composed of adipose-derived stem cells and two polymers was developed for construction of biodegradable and bone-promoting tissue-engineered bone. A series of composite scaffold materials with different physical properties was prepared and studied. The composite scaffold showed good biodegradability and water absorption, and exhibited excellent ability to promote bone differentiation – that is, bone defect repair function. This kind of biodegradable scaffold is expected to be applied to the field of bone repair or bone tissue engineering.

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Reference

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Vol. 17, No. 9

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Received 15 March 2022

Accepted 17 May 2022

Published online 15 June 2022

Published in print September 2022

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Author contributions

E Guo was responsible for study conception and design, obtaining funding and drafting the manuscript. J Wu was responsible for data acquisition. H Lu was responsible for data analysis and drafting the manuscript. L Wang was responsible for data analysis. Q Chen was responsible for revision of the manuscript.

Financial & competing interests disclosure

This work was supported by the Foundation of Science & Technology, Department of Zhejiang Province, P.R.China, under grant LGF19H060010, by the Foundation of Health & Family Planning Commission of Zhejiang Province, P.R. China under grant 2021KY445 and by the Foundation of Department of Education of Zhejiang Province, P.R.China under grant Y202044794. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

This study was approved by the Institutional Animal Care and Use Committee of Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College and was followed the principles of the Declaration of Helsinki.

Availability of data and material

The results and data generated during this research are all included in this article and can be obtained from the corresponding author or the first author.

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