Research Article

Utilizing platelet-rich fibrin bioscaffold at the graft site improves the structure and function of mice ovarian grafts

Department of Biology, Faculty of Science, Arak University, Arak 381-5688138, Iran

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Malek Soleimani Mehranjani

*Author for correspondence: Tel.: +98 918 161 7098; Fax: +98 863 417 3406;

E-mail Address: m-Soleimani@araku.ac.ir

Department of Biology, Faculty of Science, Arak University, Arak 381-5688138, Iran

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Seyed MA Shariatzadeh

Department of Biology, Faculty of Science, Arak University, Arak 381-5688138, Iran

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Published Online:12 Jun 2019https://doi.org/10.2217/rme-2018-0050

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Abstract

Aim: The effect of platelet-rich fibrin (PRF) bioscaffold on the structure and function of mice-autotransplanted ovaries was investigated. Materials & methods: Mice were divided into three groups: control, autografted and autografted + PRF bioscaffold. Angiogenesis, ovary histology and serum biochemical factors were assessed. Results: The total volume of the ovary, the number of follicles and the level of superoxide dismutase activity, total antioxidant capacity, IL-10, progesterone and estradiol were significantly higher in the autografted + PRF bioscaffold group compared with the autografted group. In the autografted + PRF bioscaffold group, angiogenesis was accelerated and apoptosis rate, IL-6, TNF-α, malondialdehyde concentrations were significantly lower compared with the autografted group. Conclusion: PRF bioscaffold improves the structure and function of mice-autografted ovary.

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Vol. 14, No. 5

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History

Received 20 April 2019

Accepted 4 April 2019

Published online 12 June 2019

Published in print May 2019

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Acknowledgments

The authors thank Mehdi Farahani and Samira Nadery for their Technical guidance during this research and the Arak University for its financial support with the grant number of 94/9331.

Financial & competing interests disclosure

The Arak University financially supported this research with the grant number of 94/9331. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

All of the experimental procedures carried out in this study were promoted by the ethical committee of Arak medical science university (IR.ARAKU.REC.1395.78).

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