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Research Article

Micro-Raman study of the role of sterilization on carbon nanotubes for biomedical applications

    Stefano Bellucci

    † Author for correspondence

    INFN-Laboratori Nazionali di Frascati, Via E. Fermi 40, I-00044 Frascati, Italy.

    ,
    Massimo Chiaretti

    INFN-Laboratori Nazionali di Frascati, Via E. Fermi 40, I-00044 Frascati, Italy.

    General Surgery Department ‘Paride Stefanini’, Rome University “La Sapienza”, P.za A. Moro 5, I-00185 Roma, Italy.

    ,
    Pasquale Onorato

    INFN-Laboratori Nazionali di Frascati, Via E. Fermi 40, I-00044 Frascati, Italy.

    Physics Department ‘A. Volta’, Pavia University, Via Bassi 6, I-27100 Pavia, Italy.

    ,
    Francesco Rossella

    Physics Department ‘A. Volta’, Pavia University, Via Bassi 6, I-27100 Pavia, Italy.

    ,
    Marco Simone Grandi

    Physics Department ‘A. Volta’, Pavia University, Via Bassi 6, I-27100 Pavia, Italy.

    ,
    Pietro Galinetto

    Physics Department ‘A. Volta’, Pavia University, Via Bassi 6, I-27100 Pavia, Italy.

    ,
    Immacolata Sacco

    INFN-Laboratori Nazionali di Frascati, Via E. Fermi 40, I-00044 Frascati, Italy.

    &
    Federico Micciulla

    INFN-Laboratori Nazionali di Frascati, Via E. Fermi 40, I-00044 Frascati, Italy.

    Published Online:https://doi.org/10.2217/nnm.09.100

    Aim: We investigate the effect of four different types of sterilization procedures on the structural properties and morphological features of single-wall carbon nanotube samples approachable by micro-Raman spectroscopy. Sterilization procedures (treatment in humid heat autoclave or ethylene oxide and irradiation with γ-rays or UV light) are necessary in view of the use of carbon nanotube sterile samples in in vivo toxicity tests on laboratory rats. Micro-Raman spectroscopy allows us to estimate several details about the morphology of the single-wall carbon nanotube mixture (mainly the presence of disorder and diameter distribution) before and after the sterilization treatment. Results: The best of these treatments, in other words, the one that least affected the morphology and structural properties of carbon nanotubes, was found to be UV irradiation and has thus been selected for future in vivo tests on rats.

    Papers of special note have been highlighted as: ▪ of interest

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