Research Article

Chemically synthesized ciprofloxacin-PEG-FeO nanotherapeutic exhibits strong antibacterial and controlled cytotoxic effects

Department of Microbiology, University of Haripur, Khyber Pakhtunkhwa, 22620, Pakistan

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Sobia Nisa

*Author for correspondence:

E-mail Address: sobia@uoh.edu.pk

https://orcid.org/0000-0002-4541-1490

Department of Microbiology, University of Haripur, Khyber Pakhtunkhwa, 22620, Pakistan

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Syeda Asma Bano

Department of Microbiology, University of Haripur, Khyber Pakhtunkhwa, 22620, Pakistan

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Muhammad Zia

Department of Biotechnology, Quaid e Azam University Islamabad, Islamabad, 15320, Pakistan

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Published Online:26 Mar 2024https://doi.org/10.2217/nnm-2023-0298

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Abstract

Aim: To develop a biocompatible conjugated ciprofloxacin-PEG-FeO nanodelivery system with increased efficacy of available therapeutics in a controlled manner. Materials & methods: FeO nanoparticles were synthesized by chemical and biological methods and modified as ciprofloxacin-PEG-FeO nanoformulations. After initial antibacterial and cytotoxicity studies, the effective and biocompatible nanoformulations was further fabricated as nanotherapeutics for in vivo studies in mouse models. Results: Chemically synthesized ciprofloxacin-PEG-FeO nanoformulations demonstrated boosted antibacterial activity against clinically isolated bacterial strains. Nanoformulations were also found to be compatible with baby hamster kidney 21 cells and red blood cells. In in vivo studies, nanotherapeutic showed wound-healing effects with eradication of Staphylococcus aureus infection. Conclusion: The investigations indicate that the developed nanotherapeutic can eradicate localized infections and enhance wound healing with controlled cytotoxicity.

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A ciprofloxacin-PEG-FeO nanodrug delivery system was developed, which showed enhanced antibiotic efficacy with a broad range of effects and controlled cytotoxicity in in vitro and in vivo experiments.

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Papers of special note have been highlighted as: • of interest; •• of considerable interest

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Vol. 19, No. 10

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History

Received 12 October 2023

Accepted 24 January 2024

Published online 26 March 2024

Published in print April 2024

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Supplementary data

To view the supplementary data that accompany this paper please visit the journal website at: www.futuremedicine.com/doi/suppl/10.2217/nnm-2023-0298

Author contributions

Conceptualization: S Nisa and Hussan. Methodology: S Nisa and Hussan. Software: Hussan Validation: S Nisa and M Zia. Formal analysis: Hussan. Investigation: S Nisa and M Zia. Resources: M Zia. Data interpretation: Hussan. Writing: Hussan. Review and editing: S Nisa and SA Bano. Visualization: S Nisa and M Zia. Supervision: S Nisa and M Zia. Project administration: S Nisa, SA Bano and M Zia. All authors have read and agreed to the final version of the manuscript.

Financial disclosure

The authors have no financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending or royalties.

Competing interests disclosure

The authors have no competing interests or relevant affiliations with any organization or entity with an interest in or conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending or royalties.

Writing disclosure

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The present study was approved by the departmental or institutional review board and ethical review board of the University of Haripur (Haripur, Pakistan) with registration no. (F20-1986-MIC-PhD[Micro]/UOH) and NIH ethical letter (F.1-5/ERC/2021).

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