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Research Article

Hyaluronic acid-engineered Bcl-2 inhibitor nanocrystals for site-specific delivery to breast tumor cells

    Dilip Panwar

    Pharmaceutical Nanotechnology Research Laboratory, Department of Pharmaceutics, National Institute of Pharmaceutical Education & Research (NIPER), Hyderabad, 500037, Telangana, India

    ,
    Pradip Thakor

    Pharmaceutical Nanotechnology Research Laboratory, Department of Pharmaceutics, National Institute of Pharmaceutical Education & Research (NIPER), Hyderabad, 500037, Telangana, India

    ,
    Madhu Sharma

    Division of Pharmaceutics, Council of Scientific & Industrial Research-Central Drug Research Institute (CSIR-CDRI), Lucknow, 226017, Uttar Pradesh, India

    ,
    Avijit Kumar Bakshi

    Division of Pharmaceutics, Council of Scientific & Industrial Research-Central Drug Research Institute (CSIR-CDRI), Lucknow, 226017, Uttar Pradesh, India

    ,
    Valamla Bhavana

    Pharmaceutical Nanotechnology Research Laboratory, Department of Pharmaceutics, National Institute of Pharmaceutical Education & Research (NIPER), Hyderabad, 500037, Telangana, India

    ,
    Vaibhavi Srivastava

    Pharmaceutical Nanotechnology Research Laboratory, Department of Pharmaceutics, National Institute of Pharmaceutical Education & Research (NIPER), Hyderabad, 500037, Telangana, India

    ,
    Prabhat Ranjan Mishra

    Division of Pharmaceutics, Council of Scientific & Industrial Research-Central Drug Research Institute (CSIR-CDRI), Lucknow, 226017, Uttar Pradesh, India

    ,
    Shashi Bala Singh

    Department of Biological Sciences, National Institute of Pharmaceutical Education & Research (NIPER), Hyderabad, 500037, Telangana, India

    &
    Neelesh Kumar Mehra

    *Author for correspondence: Tel.: +91 883 903 0437;

    E-mail Address: neelesh@niperhyd.ac.in

    ;

    E-mail Address: neelesh81mph@gmail.com

    Pharmaceutical Nanotechnology Research Laboratory, Department of Pharmaceutics, National Institute of Pharmaceutical Education & Research (NIPER), Hyderabad, 500037, Telangana, India

    Published Online:https://doi.org/10.2217/nnm-2023-0132

    Aim: This investigation aims to repurpose venetoclax using hyaluronic acid-coated venetoclax nanocrystals (HA-VEN-NCs) to target breast cancer. Materials & methods: An antisolvent precipitation method was used to fabricate the nanocrystals and optimize them using central composite design. Hyaluronic acid (HA)-coated and -uncoated nanocrystals were compared in terms of in vitro drug release, cell line studies, CD44-expressing breast tumor cell binding capability and anticancer activity. Results: HA-VEN-NCs and venetoclax nanocrystals (VEN-NCs) showed pH-responsive drug-release behavior, exhibiting sustained release at pH 6.8. Our extensive in vitro cell line investigation showed that HA-VEN-NCs efficiently bind to CD44-expressing breast tumor cells and possess excellent anticancer activity (IC50: 2.00 μg/ml) compared with VEN-NCs. Conclusion: Our findings anticipate that HA-VEN-NCs could serve as valuable nanoplatforms for cancer treatments in the future.

    Graphical abstract

    Papers of special note have been highlighted as: • of interest; •• of considerable interest

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