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Research Article

Towards epigenetic regulation of triple-negative breast cancer via ligand-mediated nanoparticles

    Guilherme F Picheth

    *Author for correspondence:

    E-mail Address: gfpicheth@ufpr.br

    Department of Basic Pathology, Federal University of Paraná, Curitiba, Paraná, Brazil

    Department of Biochemistry, Federal University of Paraná, Curitiba, Paraná, 8150-000, Brazil

    ,
    Gabriela C Cardoso

    Department of Basic Pathology, Federal University of Paraná, Curitiba, Paraná, Brazil

    ,
    Michelle B Collini

    Department of Basic Pathology, Federal University of Paraná, Curitiba, Paraná, Brazil

    ,
    João OC Filizzola

    Department of Basic Pathology, Federal University of Paraná, Curitiba, Paraná, Brazil

    ,
    Leonardo B Colauto

    Department of Basic Pathology, Federal University of Paraná, Curitiba, Paraná, Brazil

    ,
    Giovana G Nunes

    Department of Chemistry, Federal University of Paraná, Curitiba, Paraná, 8150-000, Brazil

    ,
    Cleverton L Pirich

    Department of Bioprocess Engineering & Biotechnology, Federal University of Paraná, Curitiba, Paraná, 8150-000, Brazil

    ,
    Thiago Alessandre da Silva

    Department of Bioprocess Engineering & Biotechnology, Federal University of Paraná, Curitiba, Paraná, 8150-000, Brazil

    ,
    Flávia LD Pontes

    Department of Biochemistry, Federal University of Paraná, Curitiba, Paraná, 8150-000, Brazil

    ,
    Ingrid F Zattoni

    School of Medicine, Pontifical Catholic University of Paraná. Curitiba, Paraná, 80215-901, Brazil

    ,
    Edneia ASR Cavalieri

    Department of Basic Pathology, Federal University of Paraná, Curitiba, Paraná, Brazil

    &
    Giseli Klassen

    **Author for correspondence:

    E-mail Address: giseli@ufpr.br

    Department of Basic Pathology, Federal University of Paraná, Curitiba, Paraná, Brazil

    Published Online:https://doi.org/10.2217/nnm-2023-0006

    Aims: Develop and analyze triple-negative breast cancer targeted nanoparticles loaded with the demethylating agent decitabine. Materials & methods: The polymers were synthesized by ring-opening polymerization of D,L-lactide and formulated into nanoparticles via emulsion-evaporation method. The nanoparticles were characterized by physicochemical analysis as well as in vitro using breast cancer cell lineages. Results & conclusion: The targeted nanoparticles exhibited a hydrodynamic diameter of 75 ± 12 nm, zeta potential -6.3 ± 0.2 mV and spherical morphology, and displayed greater in vitro accumulation into MDA-MB-231 (triple-negative breast cancer cell-line) compared with MCF7 and HB4A cell lineages as verified by fluorescence confocal microscopy and significant demethylating effects via ADAM33 screening by PCR.

    Graphical abstract

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