Research Article

Prophylactic and therapeutic role of catechin-loaded poly(D,L-lactic-co-glycolic acid) nanocapsules in gastric ulcer by in vitro and in vivo approach

*Author for correspondence: Tel.: +91 983 611 6930;

E-mail Address: anirbanroy1234@gmail.com

https://orcid.org/0000-0001-9134-6952

Department of Food Technology & Biochemical Engineering, Jadavpur University, Kolkata, West Bengal, 700032, India

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Runu Chakraborty

https://orcid.org/0000-0003-4026-2542

Department of Food Technology & Biochemical Engineering, Jadavpur University, Kolkata, West Bengal, 700032, India

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Published Online:12 Apr 2023https://doi.org/10.2217/nnm-2022-0267

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Abstract

Background: Gastric ulcer develops from imbalance of gastro-aggressive and protective factors. As existing drugs have adverse effects, use of natural products is in continuous expansion. In this study, we prepared nanoformulation with catechin and polylactide-co-glycolide to provide a sustained, controlled and targeted delivery. Materials & methods: Detailed characterization and toxicity study of nanoparticles were done on cells and Wistar rats. The comparative actions of free compound and nanocapsule were investigated in vitro and in vivo during treatment of gastric injury. Results: Nanocatechin improved bioavailability, reduced gastric damage at a significantly lower dose (2.5 mg/kg) by safeguarding from reactive oxygen species, restored mitochondrial integrity and downregulated MMP-9 and other inflammatory mediators. Conclusion: Nanocatechin is a better alternative for preventing and healing gastric ulcers.

Plain language summary

Gastric ulcer, a chronic disease, has a widespread effect on the global populace. Side effects become an issue with available drugs, so natural products are getting acceptance. A promising nanodrug has been designed with catechin, the primary component of green tea, to offer enhanced potency at a lower dose. Toxicity and efficacy studies on laboratory rats have shown its suitability for biological use. In our experimental model of gastric ulcer in rats, nanocatechin was given as drug. It showed improved absorption and relatively fast healing without any adverse impacts. Molecular-level research demonstrated its role in restoring mitochondrial integrity. Thus, it may be an alternative choice for treating stomach ulcers in the clinical setting.

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Papers of special note have been highlighted as: • of interest; •• of considerable interest

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Vol. 18, No. 3

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History

Received 14 October 2022

Accepted 20 February 2023

Published online 12 April 2023

Published in print February 2023

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Supplementary data

To view the supplementary data that accompany this paper please visit the journal website at: www.futuremedicine.com/doi/suppl/10.2217/nnm-2022-0267

Acknowledgments

The authors thankfully acknowledge the contribution of AK Roy, (Jadavpur University, Kolkata, India) for his generous help in preparing figures, formatting the manuscript and statistical analysis.

Financial & competing interests disclosure

The authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

All experimental procedures and protocols used in this study were reviewed and approved by the institutional animal ethics committee and were conducted according to Committee for the Purpose of Control and Supervision of Experiments on Animals guidelines.

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