Research Article

Near-infrared enhances antiangiogenic potentiality of quinacrine-gold hybrid nanoparticles in breast cancer stem cells via deregulation of HSP-70/TGF-β

Cancer Biology Division, School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT), Deemed to be University, Bhubaneswar, Odisha, 751024, India

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Biswajit Das

https://orcid.org/0000-0002-9112-0600

Cancer Biology Division, School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT), Deemed to be University, Bhubaneswar, Odisha, 751024, India

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Chinmay Das

https://orcid.org/0000-0003-2144-667X

Cancer Biology Division, School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT), Deemed to be University, Bhubaneswar, Odisha, 751024, India

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Saptarshi Sinha

https://orcid.org/0000-0003-0687-924X

Cancer Biology Division, School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT), Deemed to be University, Bhubaneswar, Odisha, 751024, India

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Subarno Paul

https://orcid.org/0000-0003-4290-7084

Cancer Biology Division, School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT), Deemed to be University, Bhubaneswar, Odisha, 751024, India

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Rajalaxmi Pradhan

https://orcid.org/0000-0002-2430-7790

Cancer Biology Division, School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT), Deemed to be University, Bhubaneswar, Odisha, 751024, India

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Chanakya Nath Kundu

*Author for correspondence: Tel.: +91 674 272 5466;

E-mail Address: cnkundu@kiitbiotech.ac.in

https://orcid.org/0000-0003-0297-1030

Cancer Biology Division, School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT), Deemed to be University, Bhubaneswar, Odisha, 751024, India

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Published Online:14 Mar 2023https://doi.org/10.2217/nnm-2022-0243

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Abstract

Aim: This study aimed to explore the antiangiogenic mechanism of quinacrine-gold hybrid nanoparticle (QAuNP) and near-infrared (NIR) radiation in patient-derived primary breast cancer stem cells. Materials & methods: Various cell-based in ovo angiogenesis and in vivo patient-derived xenograft mouse systems were used as models for the study. Results: The experimental results showed that QAuNP + NIR treatment deregulated the HSP-70/TGF-β physical interaction in primary breast cancer stem cells. Reduced TGF-β secretion in the tumor microenvironment inhibited angiogenesis activation in endothelial cells by deregulating the TGF-β-mediated PI3K/AKT/mTOR cascade. Conclusion: This study revealed that QAuNP + NIR irradiation downregulated HSP-70 expression, inhibited the HSP-70/TGF-β interaction, reduced the secretion of TGF-β in the tumor microenvironment and ultimately inhibited TGF-β-mediated angiogenesis.

Graphical abstract

Plain language summary

This study discovered that the formation of blood vessels in breast cancer is significantly reduced when hybrid nanoparticles and infrared laser therapy are used to treat breast cancer stem cells. The secretory cytokines in the tumor microenvironment primarily responsible for developing blood vessels in the tumor are dramatically reduced by treatment. As a result, the tumor's blood vessel growth is reduced, making it difficult for the cancer cells to get the nutrients and oxygen they need to survive.

Tweetable abstract

Near-infrared radiation enhances the antiangiogenic potentiality of quinacrine-gold hybrid nanoparticles in patient-derived breast cancer stem cells via deregulation of the HSP-70/TGF-β axis.

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Papers of special note have been highlighted as: • of interest

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Vol. 18, No. 1

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History

Received 23 September 2022

Accepted 24 January 2023

Published online 14 March 2023

Published in print January 2023

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Supplementary data

To view the supplementary data that accompany this paper please visit the journal website at: www.futuremedicine.com/doi/suppl/10.2217/nnm-2022-0243

Author contributions

SR Dash: conceptualization, methodology, investigation, validation, visualization, writing – original draft, writing – review and editing. B Das: formal analysis, validation, visualization, methodology, writing – review and editing. C Das: formal analysis, validation, visualization, methodology. S Sinha: formal analysis, validation, visualization, methodology, writing – review and editing. S Paul: resources, methodology. R Pradhan: methodology. CN Kundu: conceptualization, methodology, investigation, validation, visualization, project administration, supervision, funding acquisition, writing – review and editing.

Financial & competing interests disclosure

This work was financially supported by the Department of Science and Technology (DST) INSPIRE and Indian Council of Medical Research (ICMR; ref no. 5/13/57/2015/-NCD-III). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical disclosure

The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.

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