Research Article

Department of Biological Sciences, University of Calgary, Calgary, AB, T2N 1N4, Canada

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Patrick Lai

https://orcid.org/0000-0002-5679-5258

Department of Biological Sciences, University of Calgary, Calgary, AB, T2N 1N4, Canada

Current address: Rane Pharmaceuticals, Edmonton, AB, T6E 5V2, Canada

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Raimar Loebenberg

https://orcid.org/0000-0002-0919-0213

Department of Pharmacy & Pharmaceutical Sciences, University of Alberta, Edmonton, AB, T6G 2E3, Canada

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Elmar J Prenner

*Author for correspondence:

E-mail Address: eprenner@ucalgary.ca

https://orcid.org/0000-0002-4821-2657

Department of Biological Sciences, University of Calgary, Calgary, AB, T2N 1N4, Canada

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Published Online:28 Feb 2023https://doi.org/10.2217/nnm-2022-0232

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Abstract

Aims: Vaping has given rise to e-cigarette or vaping product use-associated lung injury. Model lung surfactant films were used to assess the impact of vape additives (vitamin E, vitamin E acetate, tetrahydrocannabinol, cannabidiol). This work builds upon our previous findings, by incorporating cholesterol, to understand the interplay between the additives and the sterol in surfactant function. Materials & methods: Compression–expansion cycles of lipid monofilm at the air–water interface and Brewster angle microscopy allowed elucidating the effects of vape additives. Results & conclusion: Vape additives at 5 mol% inhibited proper lipid packing and reduced film stability. Cholesterol enhanced the additive effects, resulting in significantly destabilized films and altered domains. The observed impact could signify dysfunctional lung surfactant and impaired lung function.

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Papers of special note have been highlighted as: • of interest; •• of considerable interest

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Vol. 17, No. 30

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History

Received 6 September 2022

Accepted 13 January 2023

Published online 28 February 2023

Published in print December 2022

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Supplementary data

To view the supplementary data that accompany this paper please visit the journal website at: www.futuremedicine.com/doi/suppl/10.2217/nnm-2022-0232

Author contributions

N van Bavel performed the experiments, data analysis and write-up; P Lai, data analysis and write-up; R Loebenberg, experiment design, data analysis and write-up and EJ Prenner, funding, experiment design, data analysis and write-up.

Financial & competing interests disclosure

Funding was provided by Natural Sciences and Engineering Research Council of Canada discovery grant to EJ Prenner. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

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Cholesterol enhances the negative impact of vaping additives on lung surfactant model systems

Abstract

Graphical abstract

References