Research Article

CICS-UBI – Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Avenida Infante D Henrique, Covilhã, 6200–506, Portugal

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Rita Lima-Sousa

https://orcid.org/0000-0003-4764-1967

CICS-UBI – Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Avenida Infante D Henrique, Covilhã, 6200–506, Portugal

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Bruna L Melo

https://orcid.org/0000-0002-0078-1877

CICS-UBI – Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Avenida Infante D Henrique, Covilhã, 6200–506, Portugal

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Paula Ferreira

https://orcid.org/0000-0003-3393-4427

CIEPQPF – Departamento de Engenharia Química, Universidade de Coimbra, Rua Sílvio Lima, Coimbra, 3030–790, Portugal

Department of Chemical & Biological Engineering, Coimbra Institute of Engineering (ISEC), Rua Pedro Nunes, Coimbra, 3030–199, Portugal

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André F Moreira

https://orcid.org/0000-0002-0604-2506

CICS-UBI – Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Avenida Infante D Henrique, Covilhã, 6200–506, Portugal

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Ilídio J Correia

*Author for correspondence: Tel.: +351 275 329 002;

E-mail Address: icorreia@ubi.pt

https://orcid.org/0000-0003-1613-9675

CICS-UBI – Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Avenida Infante D Henrique, Covilhã, 6200–506, Portugal

CIEPQPF – Departamento de Engenharia Química, Universidade de Coimbra, Rua Sílvio Lima, Coimbra, 3030–790, Portugal

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Duarte de Melo-Diogo

**Author for correspondence:

E-mail Address: demelodiogo@fcsaude.ubi.pt

https://orcid.org/0000-0001-9984-3603

CICS-UBI – Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Avenida Infante D Henrique, Covilhã, 6200–506, Portugal

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Published Online:21 Feb 2023https://doi.org/10.2217/nnm-2022-0218

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Abstract

Aims: To address the limitations of IR780 by preparing hydrophilic polymer–IR780 conjugates and to employ these conjugates in the assembly of nanoparticles (NPs) intended for cancer photothermal therapy. Materials & methods: The cyclohexenyl ring of IR780 was conjugated for the first time with thiol-terminated poly(2-ethyl-2-oxazoline) (PEtOx). This novel poly(2-ethyl-2-oxazoline)-IR780 (PEtOx-IR) conjugate was combined with D-α-tocopheryl succinate (TOS), leading to the assembly of mixed NPs (PEtOx-IR/TOS NPs). Results: PEtOx-IR/TOS NPs displayed optimal colloidal stability as well as cytocompatibility in healthy cells at doses within the therapeutic range. In turn, the combination of PEtOx-IR/TOS NPs and near-infrared light reduced heterotypic breast cancer spheroid viability to just 15%. Conclusion: PEtOx-IR/TOS NPs are promising agents for breast cancer photothermal therapy.

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Conventional anticancer approaches are often associated with severe side effects. Herein, the authors assembled a novel nanoparticle whose therapeutic effect is triggered by laser light. In in vitro assays, the produced nanomaterial was able to, after interacting with laser light, reduce the viability of classic and advanced cancer models. In these conditions, but in the absence of laser light, no cytotoxicity was observed. In this way, the on-demand effect (triggered by laser light) may contribute to reduced side effects. Moreover, the produced nanoparticle revealed good stability, which is important for its future translation.

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Papers of special note have been highlighted as: • of interest; •• of considerable interest

References

1. Zhao L, Xing Y, Wang R, Yu F, Yu F. Self-assembled nanomaterials for enhanced phototherapy of cancer. ACS Appl. Bio Mater. 3(1), 86–106 (2019).
MedlineGoogle Scholar
2. Li X, Zhong S, Zhang C, Li P, Ran H, Wang Z. MAGE-targeted gold nanoparticles for ultrasound imaging-guided phototherapy in melanoma. Biomed Res. Int. 2020, 6863231 (2020).
MedlineGoogle Scholar
3. Curcio A, Silva AK, Cabana S et al. Iron oxide nanoflowers @ CuS hybrids for cancer tri-therapy: interplay of photothermal therapy, magnetic hyperthermia and photodynamic therapy. Theranostics 9(5), 1288 (2019).
Medline CASGoogle Scholar
4. Leitão MM, de Melo‐Diogo D, Alves CG, Lima‐Sousa R, Correia IJ. Prototypic heptamethine cyanine incorporating nanomaterials for cancer phototheragnostic. Adv. Healthc. Mater. 9(6), 1901665 (2020). •• Summarizes the potential of IR780 (IR)-based nanomaterials in cancer photothermal/photodynamic therapy.
CASGoogle Scholar
5. Vankayala R, Hwang KC. Near‐infrared‐light‐activatable nanomaterial‐mediated phototheranostic nanomedicines: an emerging paradigm for cancer treatment. Adv. Mater. 30(23), 1706320 (2018).
Google Scholar
6. Wang H, Li X, Tse BW-C et al. Indocyanine green-incorporating nanoparticles for cancer theranostics. Theranostics 8(5), 1227 (2018).
Medline CASGoogle Scholar
7. Alves CG, Lima-Sousa R, Melo BL, Moreira AF, Correia IJ, de Melo-Diogo D. Heptamethine cyanine-loaded nanomaterials for cancer immuno-photothermal/photodynamic therapy: a review. Pharmaceutics 14(5), 1015 (2022).
Medline CASGoogle Scholar
8. Egloff-Juras C, Bezdetnaya L, Dolivet G, Lassalle H-P. NIR fluorescence-guided tumor surgery: new strategies for the use of indocyanine green. Int. J. Nanomed. 14, 7823 (2019).
MedlineGoogle Scholar
9. Alves CG, de Melo-Diogo D, Lima-Sousa R, Costa EC, Correia IJ. Hyaluronic acid functionalized nanoparticles loaded with IR780 and DOX for cancer chemo-photothermal therapy. Eur. J. Pharm. Biopharm. 137, 86–94 (2019). • Explores the chemo-photothermal capacity of IR and doxorubicin-loaded nanoparticles (NPs).
Medline CASGoogle Scholar
10. Lin T, Yuan A, Zhao X et al. Self-assembled tumor-targeting hyaluronic acid nanoparticles for photothermal ablation in orthotopic bladder cancer. Acta Biomater. 53(15), 427–438 (2017).
Medline CASGoogle Scholar
11. Qiu X, Xu L, Zhang Y et al. Photothermal ablation of in situ renal tumor by PEG-IR780-C13 micelles and near-infrared irradiation. Mol. Pharm. 13(3), 829–838 (2016).
Medline CASGoogle Scholar
12. de Oliveira MA, Machado MGC, Silva SED et al. IR780–polymer conjugates for stable near-infrared labeling of biodegradable polyester-based nanocarriers. Eur. Polym. J. 120, 109255 (2019).
CASGoogle Scholar
13. Yuan A, Qiu X, Tang X, Liu W, Wu J, Hu Y. Self-assembled PEG-IR-780-C13 micelle as a targeting, safe and highly-effective photothermal agent for in vivo imaging and cancer therapy. Biomaterials 51, 184–193 (2015).
Medline CASGoogle Scholar
14. Wan G, Cheng Y, Song J et al. Nucleus-targeting near-infrared nanoparticles based on TAT peptide-conjugated IR780 for photo-chemotherapy of breast cancer. Chem. Eng. J. 380(15), 122458 (2020).
CASGoogle Scholar
15. Nicolas J. Drug-initiated synthesis of polymer prodrugs: combining simplicity and efficacy in drug delivery. Chem. Mater. 28(6), 1591–1606 (2016).
Medline CASGoogle Scholar
16. Manandhar S, Sjöholm E, Bobacka J, Rosenholm JM, Bansal KK. Polymer–drug conjugates as nanotheranostic agents. J. Nanotheranostics 2(1), 63–81 (2021).
Google Scholar
17. Ekladious I, Colson YL, Grinstaff MW. Polymer–drug conjugate therapeutics: advances, insights and prospects. Nat. Rev. Drug Discov. 18(4), 273–294 (2019).
Medline CASGoogle Scholar
18. Novy Z, Lobaz V, Vlk M et al. Head-to-head comparison of biological behavior of biocompatible polymers poly (ethylene oxide), poly (2-ethyl-2-oxazoline) and poly [N-(2-hydroxypropyl) methacrylamide] as coating materials for hydroxyapatite nanoparticles in animal solid tumor model. Nanomaterials (Basel) 10(9), 1690 (2020). •• Reports the in vivo behavior of NPs coated with poly(2-ethyl-2-oxazoline).
Medline CASGoogle Scholar
19. Bludau H, Czapar AE, Pitek AS, Shukla S, Jordan R, Steinmetz NF. POxylation as an alternative stealth coating for biomedical applications. Eur. Polym. J. 88, 679–688 (2017).
Medline CASGoogle Scholar
20. Koshkina O, Westmeier D, Lang T et al. Tuning the surface of nanoparticles: impact of poly (2‐ethyl‐2‐oxazoline) on protein adsorption in serum and cellular uptake. Macromol. Biosci. 16(9), 1287–1300 (2016).
Medline CASGoogle Scholar
21. Fam SY, Chee CF, Yong CY, Ho KL, Mariatulqabtiah AR, Tan WS. Stealth coating of nanoparticles in drug-delivery systems. Nanomaterials (Basel) 10(4), 787 (2020).
Medline CASGoogle Scholar
22. Xu H, Zhang W, Li Y et al. The bifunctional liposomes constructed by poly(2-ethyl-oxazoline)-cholesteryl methyl carbonate: an effectual approach to enhance liposomal circulation time, pH-sensitivity and endosomal escape. Pharm. Res. 31(11), 3038–3050 (2014).
Medline CASGoogle Scholar
23. Gao N, Xing C, Wang H et al. pH-responsive dual drug-loaded nanocarriers based on poly (2-ethyl-2-oxazoline) modified black phosphorus nanosheets for cancer chemo/photothermal therapy. Front. Pharmacol. 10, 270 (2019).
Medline CASGoogle Scholar
24. Abu Lila AS, Kiwada H, Ishida T. The accelerated blood clearance (ABC) phenomenon: clinical challenge and approaches to manage. J. Control. Rel. 172(1), 38–47 (2013).
MedlineGoogle Scholar
25. d'Avanzo N, Celia C, Barone A et al. Immunogenicity of polyethylene glycol based nanomedicines: mechanisms, clinical implications and systematic approach. Adv. Ther. 3(3), 1900170 (2020).
Google Scholar
26. Luo N, Weber JK, Wang S et al. PEGylated graphene oxide elicits strong immunological responses despite surface passivation. Nat. Commun. 8(1), 1–10 (2017).
MedlineGoogle Scholar
27. Pais-Silva C, de Melo-Diogo D, Correia IJ. IR780-loaded TPGS-TOS micelles for breast cancer photodynamic therapy. Eur. J. Pharm. Biopharm. 113, 108–117 (2017). • Analyzes the effect of D-α-tocopheryl succinate (TOS) in the assembly of IR-incorporating nanomaterials.
Medline CASGoogle Scholar
28. Tsai M-H, Peng C-L, Yang S-J, Shieh M-J. Photothermal, targeting, theranostic near-infrared nanoagent with SN38 against colorectal cancer for chemothermal therapy. Mol. Pharm. 14(8), 2766–2780 (2017).
Medline CASGoogle Scholar
29. Alves CG, de Melo-Diogo D, Lima-Sousa R, Correia IJ. IR780 loaded sulfobetaine methacrylate-functionalized albumin nanoparticles aimed for enhanced breast cancer phototherapy. Int. J. Pharm. 582(30), 119346 (2020).
Medline CASGoogle Scholar
30. Mó I, Alves CG, de Melo‐Diogo D, Lima‐Sousa R, Correia IJ. Assessing the combinatorial chemo‐photothermal therapy mediated by sulfobetaine methacrylate‐functionalized nanoparticles in 2D and 3D in vitro cancer models. Biotechnol. J. 15(12), 2000219 (2020).
CASGoogle Scholar
31. Lima-Sousa R, de Melo-Diogo D, Alves CG et al. Hyaluronic acid functionalized green reduced graphene oxide for targeted cancer photothermal therapy. Carbohydr. Polym. 200, 93–99 (2018).
Medline CASGoogle Scholar
32. de Melo-Diogo D, Lima-Sousa R, Alves CG, Correia IJ. Graphene family nanomaterials for application in cancer combination photothermal therapy. Biomater. Sci. 7(9), 3534–3551 (2019).
Medline CASGoogle Scholar
33. Mó I, Sabino IJ, de Melo-Diogo D, Lima-Sousa R, Alves CG, Correia IJ. The importance of spheroids in analyzing nanomedicine efficacy. Nanomedicine (Lond.) 15(15), 1513–1525 (2020).
CASGoogle Scholar
34. Lu H, Stenzel MH. Multicellular tumor spheroids (MCTS) as a 3D in vitro evaluation tool of nanoparticles. Small 14(13), 1702858 (2018).
Google Scholar
35. Barreras-Urbina CG, Ramírez-Wong B, López-Ahumada GA et al. Nano- and micro-particles by nanoprecipitation: possible application in the food and agricultural industries. Int. J. Food Prop. 19(9), 1912–1923 (2016).
Google Scholar
36. Martínez Rivas CJ, Tarhini M, Badri W et al. Nanoprecipitation process: from encapsulation to drug delivery. Int. J. Pharm. 532(1), 66–81 (2017).
MedlineGoogle Scholar
37. Blanco E, Shen H, Ferrari M. Principles of nanoparticle design for overcoming biological barriers to drug delivery. Nat. Biotechnol. 33(9), 941 (2015).
Medline CASGoogle Scholar
38. Vu-Quang H, Vinding MS, Nielsen T et al. Pluronic F127–folate coated super paramagenic iron oxide nanoparticles as contrast agent for cancer diagnosis in magnetic resonance imaging. Polymers (Basel) 11(4), 743 (2019).
Medline CASGoogle Scholar
39. Chen M, Bhattarai N, Cong M, Pérez RL, McDonough KC, Warner IM. Mitochondria targeting IR780-based nanoGUMBOS for enhanced selective toxicity towards cancer cells. RSC Adv. 8(55), 31700–31709 (2018).
Medline CASGoogle Scholar
40. Tan Y, Zhu Y, Wen L et al. Mitochondria-responsive drug release along with heat shock mediated by multifunctional glycolipid micelles for precise cancer chemo-phototherapy. Theranostics 9(3), 691–707 (2019).
Medline CASGoogle Scholar
41. Li Y, Kröger M, Liu WK. Shape effect in cellular uptake of PEGylated nanoparticles: comparison between sphere, rod, cube and disk. Nanoscale 7(40), 16631–16646 (2015).
Medline CASGoogle Scholar
42. Dias DR, Moreira AF, Correia IJ. The effect of the shape of gold core–mesoporous silica shell nanoparticles on the cellular behavior and tumor spheroid penetration. J. Mater. Chem. B 4(47), 7630–7640 (2016).
Medline CASGoogle Scholar
43. Chithrani DB. Intracellular uptake, transport, and processing of gold nanostructures. Mol. Membr. Biol. 27(7), 299–311 (2010).
Medline CASGoogle Scholar
44. Zhang K, Fang H, Chen Z, Taylor J-SA, Wooley KL. Shape effects of nanoparticles conjugated with cell-penetrating peptides (HIV Tat PTD) on CHO cell uptake. Bioconjug. Chem. 19(9), 1880–1887 (2008).
Medline CASGoogle Scholar
45. Zhao J, Lu H, Wong S, Lu M, Xiao P, Stenzel MH. Influence of nanoparticle shapes on cellular uptake of paclitaxel loaded nanoparticles in 2D and 3D cancer models. Polym. Chem. 8(21), 3317–3326 (2017).
CASGoogle Scholar
46. Ernsting MJ, Murakami M, Roy A, Li S-D. Factors controlling the pharmacokinetics, biodistribution and intratumoral penetration of nanoparticles. J. Control. Rel. 172(3), 782–794 (2013).
Medline CASGoogle Scholar
47. Hsiue G-H, Wang C-H, Lo C-L, Wang C-H, Li J-P, Yang J-L. Environmental-sensitive micelles based on poly (2-ethyl-2-oxazoline)-b-poly (L-lactide) diblock copolymer for application in drug delivery. Int. J. Pharm. 317(1), 69–75 (2006).
Medline CASGoogle Scholar
48. Yang G, Xu L, Xu J et al. Smart nanoreactors for pH-responsive tumor homing, mitochondria-targeting, and enhanced photodynamic-immunotherapy of cancer. Nano Lett. 18(4), 2475–2484 (2018).
Medline CASGoogle Scholar
49. de Melo‐Diogo D, Pais‐Silva C, Dias DR, Moreira AF, Correia IJ. Strategies to improve cancer photothermal therapy mediated by nanomaterials. Adv. Healthc. Mater. 6(10), 1700073 (2017).
Google Scholar
50. Dai L, Li K, Li M et al. Size/charge changeable acidity-responsive micelleplex for photodynamic-improved PD-L1 immunotherapy with enhanced tumor penetration. Adv. Funct. Mater. 28(18), 1707249 (2018).
Google Scholar
51. Duan X, Li Y. Physicochemical characteristics of nanoparticles affect circulation, biodistribution, cellular internalization, and trafficking. Small 9(9–10), 1521–1532 (2013).
Medline CASGoogle Scholar
52. Kulbacka J, Pucek A, Kotulska M et al. Electroporation and lipid nanoparticles with cyanine IR-780 and flavonoids as efficient vectors to enhanced drug delivery in colon cancer. Bioelectrochemistry 110, 19–31 (2016).
Medline CASGoogle Scholar
53. Lima-Sousa R, Alves CG, Melo BL et al. Poly(2-ethyl-2-oxazoline) functionalized reduced graphene oxide: optimization of the reduction process using dopamine and application in cancer photothermal therapy. Mater. Sci. Eng. C Mater. Biol. Appl. 130, 112468 (2021).
Medline CASGoogle Scholar
54. Wang K, Zhang Y, Wang J et al. Self-assembled IR780-loaded transferrin nanoparticles as an imaging, targeting and PDT/PTT agent for cancer therapy. Sci. Rep. 6(1), 1–11 (2016).
MedlineGoogle Scholar
55. Jiang C, Cheng H, Yuan A, Tang X, Wu J, Hu Y. Hydrophobic IR780 encapsulated in biodegradable human serum albumin nanoparticles for photothermal and photodynamic therapy. Acta Biomater. 14(1), 61–69 (2015).
Medline CASGoogle Scholar
56. Kuang Y, Zhang K, Cao Y et al. Hydrophobic IR-780 dye encapsulated in cRGD-conjugated solid lipid nanoparticles for NIR imaging-guided photothermal therapy. ACS Appl. Mater. Interfaces 9(14), 12217–12226 (2017).
Medline CASGoogle Scholar
57. Potara M, Nagy-Simon T, Focsan M et al. Folate-targeted pluronic–chitosan nanocapsules loaded with IR780 for near-infrared fluorescence imaging and photothermal–photodynamic therapy of ovarian cancer. Colloids Surf. B Biointerfaces 203, 111755 (2021).
Medline CASGoogle Scholar
58. Liu G, Lin T, Zhang Q et al. Hyaluronic acid–IR780 nanoparticles for photothermal ablation in orthotopic renal cancer. J. Nanomater. 2020, 2421971 (2020).
Google Scholar
59. Chen Y, Li Z, Wang H et al. IR-780 loaded phospholipid mimicking homopolymeric micelles for near-IR imaging and photothermal therapy of pancreatic cancer. ACS Appl. Mater. Interfaces 8(11), 6852–6858 (2016).
Medline CASGoogle Scholar
60. Guo F, Yu M, Wang J, Tan F, Li N. The mitochondria-targeted and IR780-regulated theranosomes for imaging and enhanced photodynamic/photothermal therapy. RSC Adv. 6(14), 11070–11076 (2016).
CASGoogle Scholar
61. Zhang C, Liu T, Su Y et al. A near-infrared fluorescent heptamethine indocyanine dye with preferential tumor accumulation for in vivo imaging. Biomaterials 31(25), 6612–6617 (2010).
Medline CASGoogle Scholar
62. Duhem N, Danhier F, Préat V. Vitamin E-based nanomedicines for anti-cancer drug delivery. J. Control. Rel. 182, 33–44 (2014).
Medline CASGoogle Scholar
63. Constantinou C, Papas A, Constantinou AI. Vitamin E and cancer: an insight into the anticancer activities of vitamin E isomers and analogs. Int. J. Cancer 123(4), 739–752 (2008).
Medline CASGoogle Scholar
64. Meyenberg A, Goldblum D, Zingg J-M et al. Tocotrienol inhibits proliferation of human Tenon's fibroblasts in vitro: a comparative study with vitamin E forms and mitomycin C. Graefes Arch. Clin. Exp. Ophthalmol. 243(12), 1263–1271 (2005).
Medline CASGoogle Scholar
65. Gupta AK, Wells S. Surface-modified superparamagnetic nanoparticles for drug delivery: preparation, characterization, and cytotoxicity studies. IEEE Trans. Nanobiosci. 3(1), 66–73 (2004).
MedlineGoogle Scholar
66. Danhier F, Kouhé TTB, Duhem N et al. Vitamin E-based micelles enhance the anticancer activity of doxorubicin. Int. J. Pharm. 476(1–2), 9–15 (2014).
Medline CASGoogle Scholar
67. Liu M, Zhang P, Deng L et al. IR780-based light-responsive nanocomplexes combining phase transition for enhancing multimodal imaging-guided photothermal therapy. Biomater. Sci. 7(3), 1132–1146 (2019).
Medline CASGoogle Scholar
68. Song J, Zhang N, Zhang L et al. IR780-loaded folate-targeted nanoparticles for near-infrared fluorescence image-guided surgery and photothermal therapy in ovarian cancer. Int. J. Nanomed. 14, 2757 (2019).
Medline CASGoogle Scholar
69. Shao J, Liang R, Ding D et al. A smart multifunctional nanoparticle for enhanced near-infrared image-guided photothermal therapy against gastric cancer. Int. J. Nanomed. 16, 2897–2915 (2021).
MedlineGoogle Scholar
70. Wang X, Xuan Z, Zhu X, Sun H, Li J, Xie Z. Near-infrared photoresponsive drug delivery nanosystems for cancer photo-chemotherapy. J. Nanobiotechnol. 18(1), 108 (2020).
MedlineGoogle Scholar
71. Li J, Pu K. Semiconducting polymer nanomaterials as near-infrared photoactivatable protherapeutics for cancer. Acc. Chem. Res. 53(4), 752–762 (2020).
Medline CASGoogle Scholar
72. Zanoni M, Piccinini F, Arienti C et al. 3D tumor spheroid models for in vitro therapeutic screening: a systematic approach to enhance the biological relevance of data obtained. Sci. Rep. 6(1), 1–11 (2016).
MedlineGoogle Scholar
73. Mehta G, Hsiao AY, Ingram M, Luker GD, Takayama S. Opportunities and challenges for use of tumor spheroids as models to test drug delivery and efficacy. J. Control. Rel. 164(2), 192–204 (2012).
Medline CASGoogle Scholar
74. Wang X, Zhen X, Wang J, Zhang J, Wu W, Jiang X. Doxorubicin delivery to 3D multicellular spheroids and tumors based on boronic acid-rich chitosan nanoparticles. Biomaterials 34(19), 4667–4679 (2013).
Medline CASGoogle Scholar
75. Zhao C, Tong Y, Li X et al. Photosensitive nanoparticles combining vascular‐independent intratumor distribution and on‐demand oxygen‐depot delivery for enhanced cancer photodynamic therapy. Small 14(12), 1703045 (2018).
Google Scholar
76. Li W, Ma J, Garcia S et al. Semishell Janus nanoparticle-enabled pH-responsive rod-shaped assembly for photothermal therapy. ACS Appl. Nano Mater. 5(1), 871–880 (2022).
CASGoogle Scholar
77. Bangde P, Pant T, Gaikwad G, Jain R, Dandekar P. Trimethyl chitosan coated palladium nanoparticles as a photothermal agent and its in vitro evaluation in 2D and 3D model of breast cancer cells. Colloids Surf. B Biointerfaces 211, 112287 (2022).
Medline CASGoogle Scholar

Vol. 17, No. 27

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History

Received 24 August 2022

Accepted 19 December 2022

Published online 21 February 2023

Published in print November 2022

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Supplementary data

To view the supplementary data that accompany this paper please visit the journal website at: www.futuremedicine.com/doi/suppl/10.2217/nnm-2022-0218

Author contributions

Investigation, formal analysis and original draft: CG Alves. Investigation, review and editing: R Lima-Sousa. Investigation, review and editing: BL Melo. Investigation: P Ferreira. Investigation, review and editing: AF Moreira. Project administration, funding acquisition, supervision, review and editing: IJ Correia. Conceptualization, investigation, supervision, review and editing: D de Melo-Diogo.

Financial & competing interests disclosure

This study was developed within the scope of Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior projects UIDB/00709/2020 and UIDP/00709/2020, financed by national funds through the Portuguese Foundation for Science and Technology/Ministry of Science, Technology and Higher Education. Funding from POCI-01-0145-FEDER-031462, PTDC/BTA-BTA/0696/2020 and 2022.06320.PTDC is also acknowledged. D de Melo-Diogo acknowledges the Foundation for Science and Technology for financial support given through a Junior Researcher contract (2021.00590.CEECIND). CG Alves, R Lima-Sousa and BL Melo acknowledge funding from individual PhD fellowships from the Foundation for Science and Technology (SFRH/BD/145386/2019, SFRH/BD/144922/2019 and 2021.06044.BD, respectively). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

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Poly(2-ethyl-2-oxazoline)–IR780 conjugate nanoparticles for breast cancer phototherapy

Abstract

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Graphical abstract

References