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Research Article

Hyperthermia and doxorubicin release by Fol-LSMO nanoparticles induce apoptosis and autophagy in breast cancer cells

    Neha Kulkarni-Dwivedi

    Nanobioscience Group, Agharkar Research Institute, Pune, 411004, Maharashtra, India

    Savitribai Phule Pune University, Pune, 411007, Maharashtra, India

    ,
    Pratikshkumar R Patel

    Polymer Science & Engineering, CSIR – National Chemical Laboratory, Pune, 411008, Maharashtra, India

    Academy of Scientific & Innovative Research, Ghaziabad, 201002, Uttar Pradesh, India

    ,
    Bhupendra V Shravage

    Savitribai Phule Pune University, Pune, 411007, Maharashtra, India

    Developmental Biology Group, Agharkar Research Institute, Pune, 411004, Maharashtra, India

    ,
    Rinku D Umrani

    LJ Institute of Pharmacy, LJ University, LJ Campus, Ahmedabad, 382210, Gujarat, India

    ,
    Kishore M Paknikar

    Nanobioscience Group, Agharkar Research Institute, Pune, 411004, Maharashtra, India

    Indian Institute of Technology, Powai, Mumbai, 400076, India

    &
    Sachin H Jadhav

    *Author for correspondence:

    E-mail Address: shjadhav@aripune.org

    Nanobioscience Group, Agharkar Research Institute, Pune, 411004, Maharashtra, India

    Savitribai Phule Pune University, Pune, 411007, Maharashtra, India

    Published Online:https://doi.org/10.2217/nnm-2022-0171

    Background: Studies on the anticancer effects of lanthanum strontium manganese oxide (LSMO) nanoparticles (NPs)-mediated hyperthermia at cellular and molecular levels are scarce. Materials & methods: LSMO NPs conjugated with folic acid (Fol-LSMO NPs) were synthesized, followed by doxorubicin-loading (DoxFol-LSMO NPs), and their effects on breast cancer cells were investigated. Results: Hyperthermia (45°C) and combination treatments exhibited the highest (∼95%) anticancer activity with increased oxidative stress. The involvement of intrinsic mitochondria-mediated apoptotic pathway and induction of autophagy was noted. Cellular and molecular evidence confirmed the crosstalk between apoptosis and autophagy, involving Beclin1, Bcl2 and Caspase-3 genes with free reactive oxygen species presence. Conclusion: The study confirmed hyperthermia and doxorubicin release by Fol-LSMO NPs induces apoptosis and autophagy in breast cancer cells.

    Graphical abstract

    Papers of special note have been highlighted as: • of interest; •• of considerable interest

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