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Research Article

Exosomal miR-196a-1 promotes gastric cancer cell invasion and metastasis by targeting SFRP1

    Chun Feng

    Department of Gastroenterology, The Third People's Hospital of Bengbu, Bengbu, 233000, PR China

    ,
    Junjun She

    Department of General Surgery, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi 710061, PR China

    ,
    Xiaobing Chen

    Department of Oncology, Henan Cancer Hospital, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, PR China

    ,
    Qunchao Zhang

    Department of Gastroenterology, The Third People's Hospital of Bengbu, Bengbu, 233000, PR China

    ,
    Xu Zhang

    Department of Gastroenterology, The Third People's Hospital of Bengbu, Bengbu, 233000, PR China

    ,
    Yongsheng Wang

    Department of Respiratory Medicine, Nanjing Drum Tower Hospital affiliated to Medical School of Nanjing University, Nanjing 210008, PR China

    ,
    Jiahui Ye

    Department of General Surgery, Nanjing Drum Tower Hospital affiliated to Medical School of Nanjing University, Nanjing 210008, PR China

    ,
    Jiajun Shi

    Department of General Surgery, Nanjing Drum Tower Hospital affiliated to Medical School of Nanjing University, Nanjing 210008, PR China

    ,
    Jinqiu Tao

    Department of General Surgery, Nanjing Drum Tower Hospital affiliated to Medical School of Nanjing University, Nanjing 210008, PR China

    ,
    Min Feng

    Department of General Surgery, Nanjing Drum Tower Hospital affiliated to Medical School of Nanjing University, Nanjing 210008, PR China

    ,
    Wenxian Guan

    Department of General Surgery, Nanjing Drum Tower Hospital affiliated to Medical School of Nanjing University, Nanjing 210008, PR China

    ,
    Hongping Xia

    ***Author for correspondence:

    E-mail Address: xiahongping@njmu.edum.cn

    Department of General Surgery, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi 710061, PR China

    Department of Pathology, School of Basic Medical Sciences & The Affiliated Sir Run Run Hospital & State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 211166, PR China

    ,
    Weijie Zhang

    **Author for correspondence:

    E-mail Address: zhangweijie1616@163.com

    Department of General Surgery, Nanjing Drum Tower Hospital affiliated to Medical School of Nanjing University, Nanjing 210008, PR China

    &
    Guifang Xu

    *Author for correspondence:

    E-mail Address: 13852293376@163.com

    Department of Gastroenterology, Nanjing Drum Tower Hospital affiliated to Medical School of Nanjing University, Nanjing 210008, PR China

    Published Online:https://doi.org/10.2217/nnm-2019-0053

    Aim: To investigate the role of exosomal miRNAs on gastric cancer (GC) metastasis. Materials & methods: miRNA expression profiles of exosomes with distinct invasion potentials were analyzed using miRNA microarray and validated by quantitative real-time PCR. In vitro and in vivo experiments assessed the role of exosomal miR-196a-1 in GC's metastasis. Results: High expression level of exosomal miR-196a-1 expression was significantly associated with poor survival in GC. Exosomes that contained miR-196a-1 were secreted from high-invasive GC cells. Ectopic miR-196a-1 expression promoted invasion of low-invasive GC cells by targeting SFRP1. Conclusion: miR-196a-1 was delivered from high-invasive GC into low-invasive GC cells via exosomes and promoted metastasis to the liver in vitro and in vivo.

    Papers of special note have been highlighted as: • of interest •• of considerable interest

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