Laboratory diagnosis of HSV and varicella zoster virus infections

Papers of special note have been highlighted as: • of interest; •• of considerable interest

References

• 1 Steiner I. Herpes virus infection of the peripheral nervous system. Handb. Clin. Neurol. 115, 543–558 (2013).
  MedlineGoogle Scholar
• 2 Norberg P. Divergence and genotyping of human alpha-herpesviruses: an overview. Infect. Genet. Evol. 10(1), 14–25 (2010).
  Medline CASGoogle Scholar
• 3 Van De Perre P, Segondy M, Foulongne V et al. Herpes simplex virus and HIV-1: deciphering viral synergy. Lancet Infect. Dis. 8(8), 490–497 (2008).
  Medline CASGoogle Scholar
• 4 Corey L, Whitley RJ, Stone EF, Mohan K. Difference between herpes simplex virus type 1 and type 2 neonatal encephalitis in neurological outcome. Lancet 1(8575–8576), 1–4 (1988).
  Medline CASGoogle Scholar
• 5 Bader MS. Herpes zoster: diagnostic, therapeutic, and preventive approaches. Postgrad. Med. 125(5), 78–91 (2013).•• One of the first papers to demonstrate the usefulness of molecular methods in HSV etiology diagnosis.
  MedlineGoogle Scholar
• 6 Gershon MD, Gershon AA. VZV infection of keratinocytes: production of cell-free infectious virions in vivo. Curr. Top. Microbiol. Immunol. 342, 173–188 (2010).
  Medline CASGoogle Scholar
• 7 Zuckerman RA, Limaye AP. Varicella zoster virus (VZV) and herpes simplex virus (HSV) in solid organ transplant patients. Am. J. Transplant. 13(Suppl. 3), 55–66 (2013).
  Medline CASGoogle Scholar
• 8 Schiffer JT, Abu-Raddad L, Mark KE et al. Frequent release of low amounts of herpes simplex virus from neurons: results of a mathematical model. Sci. Transl. Med. 1(7), 7ra16 (2009).
  MedlineGoogle Scholar
• 9 Mertz GJ, Benedetti J, Ashley R, Selke SA, Corey L. Risk factors for the sexual transmission of genital herpes. Ann. Int. Med. 116(3), 197–202 (1992).
  Medline CASGoogle Scholar
• 10 Druce J, Catton M, Chibo D et al. Utility of a multiplex PCR assay for detecting herpesvirus DNA in clinical samples. J. Clin. Microbiol. 40(5), 1728–1732 (2002).
  Medline CASGoogle Scholar
• 11 Kalman CM, Laskin OL. Herpes zoster and zosteriform herpes simplex virus infections in immunocompetent adults. Am. J. Med. 81(5), 775–778 (1986).
  Medline CASGoogle Scholar
• 12 Jain S, Wyatt D, McCaughey C, O'Neill HJ, Coyle PV. Nested multiplex polymerase chain reaction for the diagnosis of cutaneous herpes simplex and herpes zoster infections and a comparison with electronmicroscopy. J. Med. Virol. 63(1), 52–56 (2001).
  Medline CASGoogle Scholar
• 13 Sullivan M, Sams R 2nd, Jamieson B, Holt J. Clinical inquiries. What is the best test to detect herpes in skin lesions? J. Fam. Pract. 55(4), 346, 348 (2006).
  MedlineGoogle Scholar
• 14 Nahass GT, Goldstein BA, Zhu WY, Serfling U, Penneys NS, Leonardi CL. Comparison of Tzanck smear, viral culture, and DNA diagnostic methods in detection of herpes simplex and varicella–zoster infection. JAMA 268(18), 2541–2544 (1992).
  Medline CASGoogle Scholar
• 15 Frisch S, Guo AM. Diagnostic methods and management strategies of herpes simplex and herpes zoster infections. Clin. Geriat. Med. 29(2), 501–526 (2013).
  MedlineGoogle Scholar
• 16 Coffin SE, Hodinka RL. Utility of direct immunofluorescence and virus culture for detection of varicella-zoster virus in skin lesions. J. Clin. Microbiol. 33(10), 2792–2795 (1995).
  Medline CASGoogle Scholar
• 17 Zirn JR, Tompkins SD, Huie C, Shea CR. Rapid detection and distinction of cutaneous herpesvirus infections by direct immunofluorescence. J. Am. Acad. Dermatol. 33(5 Pt 1), 724–728 (1995).
  Medline CASGoogle Scholar
• 18 Anderson NW, Buchan BW, Ledeboer NA. Light microscopy, culture, molecular, and serologic methods for detection of herpes simplex virus. J. Clin. Microbiol. 52(1), 2–8 (2014).
  MedlineGoogle Scholar
• 19 Caviness AC, Oelze LL, Saz UE, Greer JM, Demmler-Harrison GJ. Direct immunofluorescence assay compared with cell culture for the diagnosis of mucocutaneous herpes simplex virus infections in children. J. Clin. Virol. 49(1), 58–60 (2010).• Excellent and state-of-the-art minireview article.
  MedlineGoogle Scholar
• 20 Chan EL, Brandt K, Horsman GB. Comparison of Chemicon SimulFluor direct fluorescent antibody staining with cell culture and shell vial direct immunoperoxidase staining for detection of herpes simplex virus and with cytospin direct immunofluorescence staining for detection of varicella-zoster virus. Clin. Diagn. Lab. Immunol. 8(5), 909–912 (2001).
  Medline CASGoogle Scholar
• 21 Brumback BG, Farthing PG, Castellino SN. Simultaneous detection of and differentiation between herpes simplex and varicella-zoster viruses with two fluorescent probes in the same test system. J. Clin. Microbiol. 31(12), 3260–3263 (1993).
  Medline CASGoogle Scholar
• 22 Ramaswamy M, McDonald C, Smith M et al. Diagnosis of genital herpes by real time PCR in routine clinical practice. Sex. Transm. Infect. 80(5), 406–410 (2004).
  Medline CASGoogle Scholar
• 23 Huang YT, Hite S, Duane V, Yan H. CV-1 and MRC-5 mixed cells for simultaneous detection of herpes simplex viruses and varicella zoster virus in skin lesions. J. Clin. Virol. 24(1–2), 37–43 (2002).
  Medline CASGoogle Scholar
• 24 Erlich KS. Laboratory diagnosis of herpesvirus infections. Clin. Lab. Med. 7(4), 759–776 (1987).
  Medline CASGoogle Scholar
• 25 Weinberg A, Clark JC, Schneider SA, Forghani B, Levin MJ. Improved detection of varicella zoster infection with a spin amplification shell vial technique and blind passage. Clin. Diagn. Virol. 5(1), 61–65 (1996).
  Medline CASGoogle Scholar
• 26 Singh A, Preiksaitis J, Ferenczy A, Romanowski B. The laboratory diagnosis of herpes simplex virus infections. Can. J. Infect. Dis. Med. Microbiol. 16(2), 92–98 (2005).
  MedlineGoogle Scholar
• 27 Leland DS, Ginocchio CC. Role of cell culture for virus detection in the age of technology. Clin. Microbiol. Rev. 20(1), 49–78 (2007).•• Excellent and comprehensive review article.
  Medline CASGoogle Scholar
• 28 Miller NS, Yen-Lieberman B, Poulter MD, Tang YW, Granato PA. Comparative clinical evaluation of the IsoAmp^® HSV Assay with ELVIS^® HSV culture/ID/typing test system for the detection of herpes simplex virus in genital and oral lesions. J. Clin. Virol. 54(4), 355–358 (2012).• Describes the first simple and rapid molecular device that has potential for point-of-care testing.
  MedlineGoogle Scholar
• 29 Patel N, Kauffmann L, Baniewicz G, Forman M, Evans M, Scholl D. Confirmation of low–titer, herpes simplex virus-positive specimen results by the enzyme-linked virus-inducible system (ELVIS) using PCR and repeat testing. J. Clin. Microbiol. 37(12), 3986–3989 (1999).
  Medline CASGoogle Scholar
• 30 Wilson DA, Yen-Lieberman B, Schindler S, Asamoto K, Schold JD, Procop GW. Should varicella-zoster virus culture be eliminated? A comparison of direct immunofluorescence antigen detection, culture, and PCR, with a historical review. J. Clin. Microbiol. 50(12), 4120–4122 (2012).
  Medline CASGoogle Scholar
• 31 Hodinka RL. Point: is the era of viral culture over in the clinical microbiology laboratory? J. Clin. Microbiol. 51(1), 2–4 (2013).• Nice comparative review of current laboratory techniques for the detection of varicella zoster virus.
  Medline CASGoogle Scholar
• 32 Guido M, Tinelli A, De Donno A et al. Susceptibility to varicella-zoster among pregnant women in the province of Lecce, Italy. J. Clin. Virol. 53(1), 72–76 (2012).
  Medline CASGoogle Scholar
• 33 Heininger U, Seward JF. Varicella. Lancet 368(9544), 1365–1376 (2006).
  MedlineGoogle Scholar
• 34 Grahn A, Studahl M, Nilsson S, Thomsson E, Backstrom M, Bergstrom T. Varicella-zoster virus (VZV) glycoprotein E is a serological antigen for detection of intrathecal antibodies to VZV in central nervous system infections, without cross-reaction to herpes simplex virus 1. Clin. Vac. Immunol. 18(8), 1336–1342 (2011).
  Medline CASGoogle Scholar
• 35 Laderman EI, Whitworth E, Dumaual E et al. Rapid, sensitive, and specific lateral-flow immunochromatographic point-of-care device for detection of herpes simplex virus type 2-specific immunoglobulin G antibodies in serum and whole blood. Clin. Vac. Immunol. 15(1), 159–163 (2008).
  Medline CASGoogle Scholar
• 36 Nagel MA, Forghani B, Mahalingam R et al. The value of detecting anti-VZV IgG antibody in CSF to diagnose VZV vasculopathy. Neurology 68(13), 1069–1073 (2007).
  Medline CASGoogle Scholar
• 37 Martins TB, Welch RJ, Hill HR, Litwin CM. Comparison of a multiplexed herpes simplex virus type-specific immunoglobulin G serology assay to immunoblot, Western blot, and enzyme-linked immunosorbent assays. Clin. Vac. Immunol. 16(1), 55–60 (2009).
  Medline CASGoogle Scholar
• 38 Sauerbrei A, Wutzler P. Novel recombinant ELISA assays for determination of type-specific IgG antibodies against HSV-1 and HSV-2. J. Virol. Meth. 144(1–2), 138–142 (2007).
  Medline CASGoogle Scholar
• 39 Gorander S, Svennerholm B, Liljeqvist JA. Secreted portion of glycoprotein g of herpes simplex virus type 2 is a novel antigen for type-discriminating serology. J. Clin. Microbiol. 41(8), 3681–3686 (2003).
  MedlineGoogle Scholar
• 40 Morrow R, Friedrich D. Performance of a novel test for IgM and IgG antibodies in subjects with culture-documented genital herpes simplex virus-1 or -2 infection. Clin. Microbiol. Infect. 12(5), 463–469 (2006).
  Medline CASGoogle Scholar
• 41 Rowley AH, Whitley RJ, Lakeman FD, Wolinsky SM. Rapid detection of herpes-simplex-virus DNA in cerebrospinal fluid of patients with herpes simplex encephalitis. Lancet 335(8687), 440–441 (1990).
  Medline CASGoogle Scholar
• 42 Tang YW, Mitchell PS, Espy MJ, Smith TF, Persing DH. Molecular diagnosis of herpes simplex virus infections in the central nervous system. J. Clin. Microbiol. 37(7), 2127–2136 (1999).•• One of the first papers to demonstrate the usefulness of molecular methods in HSV etiology diagnosis.
  Medline CASGoogle Scholar
• 43 Lemieux B, Li Y, Kong H, Tang YW. Near instrument-free, simple molecular device for rapid detection of herpes simplex viruses. Expert Rev. Mol. Diagn. 12(5), 437–443 (2012).
  Medline CASGoogle Scholar
• 44 Selvaraju SB, Wurst M, Horvat RT, Selvarangan R. Evaluation of three analyte-specific reagents for detection and typing of herpes simplex virus in cerebrospinal fluid. Diagn. Microbiol. Infect. Dis. 63(3), 286–291 (2009).• Review article that introduces and contrasts the current US FDA-approved device for HSV detection.
  Medline CASGoogle Scholar
• 45 Van Der Pol B, Warren T, Taylor SN et al. Type-specific identification of anogenital herpes simplex virus infections by use of a commercially available nucleic acid amplification test. J. Clin. Microbiol. 50(11), 3466–3471 (2012).
  Medline CASGoogle Scholar
• 46 Kim HJ, Tong Y, Tang W et al. A rapid and simple isothermal nucleic acid amplification test for detection of herpes simplex virus types 1 and 2. J. Clin. Virol. 50(1), 26–30 (2011).
  Medline CASGoogle Scholar
• 47 Fan F, Stiles J, Mikhlina A, Lu X, Babady NE, Tang YW. Clinical validation of the Lyra Direct HSV1+2/VZV Assay for simultaneous detection and differentiation of three herpes viruses in cutaneous and mucosal lesions. J. Clin. Microbiol. 52(10), 3799–3801 (2014).• Describes clinical validation results of the first US FDA-cleared multiplex PCR assay for detection and differentiation of HSV-1, HSV-2 and varicella zoster virus.
  MedlineGoogle Scholar
• 48 Gitman MR, Ferguson D, Landry ML. Comparison of Simplexa HSV 1 & 2 PCR with culture, immunofluorescence, and laboratory-developed TaqMan PCR for detection of herpes simplex virus in swab specimens. J. Clin. Microbiol. 51(11), 3765–3769 (2013).
  Medline CASGoogle Scholar
• 49 IMDx HSV-1/2 for Abbott m2000 summary. www.accessdata.fda.gov/cdrh_docs/pdf14/K140198.pdf.
  Google Scholar
• 50 Evaluation of automatic class III designation for Lyra™ Direct HSV 1 + 2/VZV assay. Decision summary. www.accessdata.fda.gov/cdrh_docs/reviews/K133448.pdf.
  Google Scholar
• 51 Kinonen CL, Gleason BC, Thomas AB, Kaul KL, Cibull TL. Dermal hypersensitivity reaction: a PCR-confirmed pattern of herpetic dermatitis. J. Cutan. Pathol. 39(10), 929–935 (2012).
  MedlineGoogle Scholar
• 52 Sankuntaw N, Sukprasert S, Engchanil C et al. Single tube multiplex real-time PCR for the rapid detection of herpesvirus infections of the central nervous system. Mol. Cell. Probes 25(2–3), 114–120 (2011).
  Medline CASGoogle Scholar
• 53 Espy MJ, Uhl JR, Sloan LM et al. Real-time PCR in clinical microbiology: applications for routine laboratory testing. Clin. Microbiol. Rev. 19(1), 165–256 (2006).
  Medline CASGoogle Scholar
• 54 Cobo F. Application of molecular diagnostic techniques for viral testing. Open Virol. J. 6, 104–114 (2012).
  MedlineGoogle Scholar
• 55 Mehta SK, Tyring SK, Gilden DH et al. Varicella-zoster virus in the saliva of patients with herpes zoster. J. Infect. Dis. 197(5), 654–657 (2008).
  MedlineGoogle Scholar
• 56 Leung J, Harpaz R, Baughman AL et al. Evaluation of laboratory methods for diagnosis of varicella. Clin. Infect. Dis. 51(1), 23–32 (2010).
  Medline CASGoogle Scholar
• 57 Tan HH, Goh CL. Viral infections affecting the skin in organ transplant recipients: epidemiology and current management strategies. Am. J. Clin. Dermatol. 7(1), 13–29 (2006).
  MedlineGoogle Scholar
• 58 Bennett S, Carman WF, Gunson RN. The development of a multiplex real-time PCR for the detection of herpes simplex virus 1 and 2, varizella zoster virus, adenovirus and Chlamydia trachomatis from eye swabs. J. Virol. Meth. 189(1), 143–147 (2013).
  Medline CASGoogle Scholar
• 59 Kannangai R, Sachithanandham J, Mahadevan A et al. Association of neurotropic viruses in HIV-infected individuals who died of secondary complications of tuberculosis, cryptococcosis, or toxoplasmosis in South India. J. Clin. Microbiol. 51(3), 1022–1025 (2013).
  MedlineGoogle Scholar
• 60 Tan TY, Zou H, Ong DC et al. Development and clinical validation of a multiplex real-time PCR assay for herpes simplex and varicella zoster virus. Diagn. Mol. Pathol. 22(4), 245–248 (2013).
  Medline CASGoogle Scholar
• 61 Buelow DR, Bankowski MJ, Fofana D, Gu Z, Pounds S, Hayden RT. Comparison of two multiplexed PCR assays for the detection of HSV-1, HSV-2, and VZV with extracted and unextracted cutaneous and mucosal specimens. J. Clin. Virol. 58(1), 84–88 (2013).
  Medline CASGoogle Scholar
• 62 Legoff J, Feghoul L, Mercier-Delarue S et al. Broad-range PCR-electrospray ionization mass spectrometry for detection and typing of adenovirus and other opportunistic viruses in stem cell transplant patients. J. Clin. Microbiol. 51(12), 4186–4192 (2013).
  Medline CASGoogle Scholar
• 63 Leveque N, Van Haecke A, Renois F, Boutolleau D, Talmud D, Andreoletti L. Rapid virological diagnosis of central nervous system infections by use of a multiplex reverse transcription-PCR DNA microarray. J. Clin. Microbiol. 49(11), 3874–3879 (2011).
  Medline CASGoogle Scholar
• 64 Sakai K, Wakasugi S, Muchemwa FC, Ihn H. Quick detection of herpes viruses from skin vesicles and exudates without nucleic acid extraction using multiplex PCR. Biosci. Trends 2(4), 164–168 (2008).
  Medline CASGoogle Scholar
• 65 Pandori MW, Lei J, Wong EH, Klausner J, Liska S. Real-time PCR for detection of herpes simplex virus without nucleic acid extraction. BMC Infect. Dis. 6, 104 (2006).
  MedlineGoogle Scholar
• 66 Binkhamis K, Al-Siyabi T, Heinstein C, Hatchette TF, Leblanc JJ. Molecular detection of varicella zoster virus while keeping an eye on the budget. J. Virol. Meth. 202, 24–27 (2014).
  Medline CASGoogle Scholar
• 67 Frobert E, Billaud G, Casalegno JS et al. The clinical interest of HSV1 semi-quantification in bronchoalveolar lavage. J. Clin. Virol. 58(1), 265–268 (2013).
  MedlineGoogle Scholar
• 68 Piret J, Boivin G. Antiviral drug resistance in herpesviruses other than cytomegalovirus. Rev. Med. Virol. 24(3), 186–218 (2014).
  Medline CASGoogle Scholar
• 69 Van Der Beek MT, Claas EC, Van Der Blij-De Brouwer CS et al. Rapid susceptibility testing for herpes simplex virus type 1 using real-time PCR. J. Clin. Virol. 56(1), 19–24 (2013).
  Medline CASGoogle Scholar
• 70 Ihira M, Higashimoto Y, Kawamura Y et al. Cycling probe technology to quantify and discriminate between wild-type varicella-zoster virus and Oka vaccine strains. J. Virol. Meth. 193(2), 308–313 (2013).
  Medline CASGoogle Scholar
• 71 Loparev VN, Argaw T, Krause PR, Takayama M, Schmid DS. Improved identification and differentiation of varicella-zoster virus (VZV) wild-type strains and an attenuated varicella vaccine strain using a VZV open reading frame 62-based PCR. J. Clin. Microbiol. 38(9), 3156–3160 (2000).
  Medline CASGoogle Scholar
• 72 Tang YW, Allawi HT, Deleon-Carnes M, Li H, Day SP, Schmid D. Detection and differentiation of wild-type and vaccine mutant varicella–zoster viruses using an Invader Plus method. J. Clin. Virol. 40(2), 129–134 (2007).
  Medline CASGoogle Scholar
• 73 Thiele S, Borschewski A, Kuchler J, Bieberbach M, Voigt S, Ehlers B. Molecular analysis of varicella vaccines and varicella-zoster virus from vaccine-related skin lesions. Clin. Vac. Immunol. 18(7), 1058–1066 (2011).
  Medline CASGoogle Scholar
• 74 Toi CS, Dwyer DE. Differentiation between vaccine and wild-type varicella-zoster virus genotypes by high-resolution melt analysis of single nucleotide polymorphisms. J. Clin. Virol. 43(1), 18–24 (2008).
  Medline CASGoogle Scholar
• 75 Schmid DS, Jumaan AO. Impact of varicella vaccine on varicella-zoster virus dynamics. Clin. Microbiol. Rev. 23(1), 202–217 (2010).
  Medline CASGoogle Scholar
• 76 Papaevangelou V, Quinlivan M, Lockwood J et al. Subclinical VZV reactivation in immunocompetent children hospitalized in the ICU associated with prolonged fever duration. Clin. Microbiol. Infect. 19(5), E245–E251 (2013).
  Medline CASGoogle Scholar
• 77 Mancuso R, Delbue S, Borghi E et al. Increased prevalence of varicella zoster virus DNA in cerebrospinal fluid from patients with multiple sclerosis. J. Med. Virol. 79(2), 192–199 (2007).
  Medline CASGoogle Scholar
• 78 Yang S, Rothman RE. PCR-based diagnostics for infectious diseases: uses, limitations, and future applications in acute-care settings. Lancet Infect. Dis. 4(6), 337–348 (2004).
  Medline CASGoogle Scholar
• 79 Caliendo AM, Gilbert DN, Ginocchio CC et al. Better tests, better care: improved diagnostics for infectious diseases. Clin. Infect. Dis. 57(Suppl. 3), S139–S170 (2013).• Provides a deep look at the general principles, diagnostic value and limitations of the most current PCR-based platforms as they evolve from bench to bedside.
  MedlineGoogle Scholar