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Short Communication

Establishment and evaluation of a multiplex PCR amplification-based sequencing method for respiratory virus type A

    Junhong Luo‡

    NHC Key laboratory of Enteric Pathogenic Microbiology, Jiangsu Province Engineering Research Center of Health Emergency, Jiangsu Provincial Center for Disease Control & Prevention, Nanjing, China

    School of Public Health, Nanjing medical university, Nanjing, China

    ‡Authors contributed equally to this study

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    ,
    Yao Huang‡

    Yangzhou City Center for Disease Control & Prevention, Yangzhou, China

    ‡Authors contributed equally to this study

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    ,
    Yan Guo‡

    Department of Neonatology, Children's Hospital of Nanjing Medical University, Nanjing, China

    ‡Authors contributed equally to this study

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    ,
    Zixinrong Meng

    School of Public Health, Nanjing medical university, Nanjing, China

    ,
    Kangchen Zhao

    NHC Key laboratory of Enteric Pathogenic Microbiology, Jiangsu Province Engineering Research Center of Health Emergency, Jiangsu Provincial Center for Disease Control & Prevention, Nanjing, China

    ,
    Xiaojuan Zhu

    NHC Key laboratory of Enteric Pathogenic Microbiology, Jiangsu Province Engineering Research Center of Health Emergency, Jiangsu Provincial Center for Disease Control & Prevention, Nanjing, China

    ,
    Le Zhou

    Yangzhou City Center for Disease Control & Prevention, Yangzhou, China

    ,
    Tao Wu

    NHC Key laboratory of Enteric Pathogenic Microbiology, Jiangsu Province Engineering Research Center of Health Emergency, Jiangsu Provincial Center for Disease Control & Prevention, Nanjing, China

    ,
    Qiao Qiao

    NHC Key laboratory of Enteric Pathogenic Microbiology, Jiangsu Province Engineering Research Center of Health Emergency, Jiangsu Provincial Center for Disease Control & Prevention, Nanjing, China

    ,
    Yiyue Ge

    NHC Key laboratory of Enteric Pathogenic Microbiology, Jiangsu Province Engineering Research Center of Health Emergency, Jiangsu Provincial Center for Disease Control & Prevention, Nanjing, China

    ,
    Qin Xu

    *Author for correspondence: Tel.: 15 855 287 0983;

    E-mail Address: 1483214000@qq.com

    Yangzhou City Center for Disease Control & Prevention, Yangzhou, China

    &
    Lunbiao Cui

    **Author for correspondence: Tel.: 86 25 83756372;

    E-mail Address: lbcui@jscdc.cn

    NHC Key laboratory of Enteric Pathogenic Microbiology, Jiangsu Province Engineering Research Center of Health Emergency, Jiangsu Provincial Center for Disease Control & Prevention, Nanjing, China

    School of Public Health, Nanjing medical university, Nanjing, China

    Published Online:https://doi.org/10.2217/fvl-2023-0083

    Aim: This study aimed to establish a simple whole-genome sequencing method for respiratory virus type A (RSVA) used in genomic epidemiological studies. Methods: Multiple primers were designed to amplify overlapping amplicons specific to RSVA. The amplicons were sequenced using Illumina and Nanopore sequencing platforms and the sequencing performances of the two platforms were also compared. Results: The study shows that the developed method could recover almost full genomes of RSVA when Ct values were less than 33 for clinical samples. Conclusion: In this study, a whole-genome sequencing method was developed for RSVA based on multiplex PCR.

    Papers of special note have been highlighted as: • of interest

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