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Division of Infectious Disease, Shenzhen Children’s Hospital, Shenzhen, 518038, China

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Lifeng Qi

*Author for correspondence: Tel.: +86 189 3869 0190;

E-mail Address: qlf0298@sina.com

https://orcid.org/0000-0003-3307-1967

Division of Infectious Disease, Shenzhen Children’s Hospital, Shenzhen, 518038, China

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Published Online:12 May 2023https://doi.org/10.2217/fvl-2023-0001

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Abstract

Aim: This study aimed to investigate the anti-adenoviral activity of stilbene derivatives from mulberry leaves. Materials & methods: The anti-adenoviral activity was tested against adenoviruses -3 and -7 on human airway epithelial cell models. Cytotoxicity was assessed by LDH assay. Adenoviral DNA was quantified by qPCR. Results: All five tested stilbene derivatives from mulberry leaves exhibited anti-adenoviral activity, with Kuwanon X showing the highest inhibitory effect. Kuwanon X showed no apparent cytotoxicity for a wide range of concentrations. The mechanistic study revealed that Kuwanon X did not affect viral entry and nuclear translocation of the adenoviral genome but reduced viral DNA production. Conclusion: Stilbene derivatives like Kuwanon X from mulberry leaves are good candidates for antiviral treatment against AdV.

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Compound from mulberry leaves shows highly inhibitory effect against human AdV.

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Papers of special note have been highlighted as: • of interest; •• of considerable interest

References

1. Fu Y, Tang Z, Ye Z et al. Human adenovirus type 7 infection causes a more severe disease than type 3. BMC Infect. Dis. 19(1), 36 (2019).
MedlineGoogle Scholar
2. Hu K, Fu M, Guan X et al. Penton base induces better protective immune responses than fiber and hexon as a subunit vaccine candidate against adenoviruses. Vaccine 36(29), 4287–4297 (2018).
Medline CASGoogle Scholar
3. Dotan M, Zion E, Bilavsky E et al. Adenovirus can be a serious, life-threatening disease, even in previously healthy children. Acta Paediatr. 111(3), 614–619 (2022).
MedlineGoogle Scholar
4. Xie L, Zhang B, Xiao N et al. Epidemiology of human adenovirus infection in children hospitalized with lower respiratory tract infections in Hunan, China. J. Med. Virol. 91(3), 392–400 (2019).
Medline CASGoogle Scholar
5. Jin Y, Zhang RF, Xie ZP et al. Prevalence of adenovirus in children with acute respiratory tract infection in Lanzhou, China. Virol. J. 10, 271 (2013).
MedlineGoogle Scholar
6. Lynch JP 3rd, Kajon AE. Adenovirus: epidemiology, global spread of novel serotypes, and advances in treatment and prevention. Semin. Respir. Crit. Care Med. 37(4), 586–602 (2016).
MedlineGoogle Scholar
7. Dodge MJ, Macneil KM, Tessier TM, Weinberg JB, Mymryk JS. Emerging antiviral therapeutics for human adenovirus infection: recent developments and novel strategies. Antivir. Res. 188, 105034 (2021).
Medline CASGoogle Scholar
8. Kuwatsuka Y, Atsuta Y, Hirakawa A et al. Use of unapproved or off-label drugs in Japan for the treatment of graft-versus-host disease and post-transplant viral infection. Int. J. Hematol. 112(6), 841–850 (2020).
Medline CASGoogle Scholar
9. Lopez SMC, Michaels MG, Green M. Adenovirus infection in pediatric transplant recipients: are effective antiviral agents coming our way? Curr. Opin. Organ Transplant. 23(4), 395–399 (2018).
MedlineGoogle Scholar
10. Wen P, Hu T-G, Linhardt RJ, Liao S-T, Wu H, Zou Y-X. Mulberry: a review of bioactive compounds and advanced processing technology. Trends Food Sci. Technol. 83, 138–158 (2019).
CASGoogle Scholar
11. Afrin S, Giampieri F, Forbes-Hernández TY et al. Manuka honey synergistically enhances the chemopreventive effect of 5-fluorouracil on human colon cancer cells by inducing oxidative stress and apoptosis, altering metabolic phenotypes and suppressing metastasis ability. Free Radic. Biol. Med. 126, 41–54 (2018).
Medline CASGoogle Scholar
12. Baranauskaite J, Kubiliene A, Marksa M et al. The influence of different oregano species on the antioxidant activity determined using HPLC postcolumn DPPH method and anticancer activity of carvacrol and rosmarinic acid. BioMed. Res. Int. 2017, 1681392 (2017).
MedlineGoogle Scholar
13. Sharma A, Kashyap D, Sak K, Tuli HS, Sharma AK. Therapeutic charm of quercetin and its derivatives: a review of research and patents. Pharm. Pat. Anal. 7(1), 15–32 (2018).
Medline CASGoogle Scholar
14. Ma G, Chai X, Hou G, Zhao F, Meng Q. Phytochemistry, bioactivities and future prospects of mulberry leaves: a review. Food Chem. 372, 131335 (2022).
Medline CASGoogle Scholar
15. Kim H-B, Lim JD, Kim A-J, Kim Y-S, Kwon O-C. Comparison with various mulberry leaves' and fruit's extract in lipid accumulation inhibitory effect at adipocyte model. Int. J. Indust. Entomol. 35(1), 1–6 (2017). • Demonstrates the inhibitory effect of extracts from mulberry leaves on a adipocyte model.
Google Scholar
16. Gryn-Rynko A, Bazylak G, Olszewska-Slonina D. New potential phytotherapeutics obtained from white mulberry (Morus alba L.) leaves. Biomed. Pharmacother. 84, 628–636 (2016).
Medline CASGoogle Scholar
17. He X, Fang J, Ruan Y et al. Structures, bioactivities and future prospective of polysaccharides from Morus alba (white mulberry): a review. Food Chem. 245, 899–910 (2018). • A review on compounds with bioactivity from mulberry leaves.
Medline CASGoogle Scholar
18. Li F, Zhang B, Chen G, Fu X. The novel contributors of anti-diabetic potential in mulberry polyphenols revealed by UHPLC-HR-ESI-TOF-MS/MS. Food Rev. Int. 100, 873–884 (2017).
Medline CASGoogle Scholar
19. Zhang D-Y, Wan Y, Hao J-Y et al. Evaluation of the alkaloid, polyphenols, and antioxidant contents of various mulberry cultivars from different planting areas in eastern China. Ind. Crops Prod. 122, 298–307 (2018).
CASGoogle Scholar
20. Ma F, Shen W, Zhang X et al. Anti-HSV activity of Kuwanon X from mulberry leaves with genes expression inhibitory and HSV-1 induced NF-κB deactivated properties. Biol. Pharm. Bull. 39(10), 1667–1674 (2016). •• Shows the antiviral activity of Kuwanon X.
Medline CASGoogle Scholar
21. Zou L, Yi L, Yu J et al. Adenovirus infection in children hospitalized with pneumonia in Guangzhou, China. Influenza Other Respir. Viruses 15(1), 27–33 (2021).
Medline CASGoogle Scholar
22. Qi L, Wang Y, Wang H, Deng J. Adenovirus 7 induces interlukin-6 expression in human airway epithelial cells via p38/NF-κB signaling pathway. Front. Immunol. 11, 551413 (2020).
Medline CASGoogle Scholar
23. Yu P, Lou D, Qi L, Chen Z. The novel antiviral properties of brassicasterol against human adenovirus. Future Virol. 16(9), 591–600 (2021). • Shows the anti-AdV activity of other natural products.
CASGoogle Scholar
24. Wang K, Huang S, Kapoor-Munshi A, Nemerow G. Adenovirus internalization and infection require dynamin. J. Virol. 72(4), 3455–3458 (1998).
Medline CASGoogle Scholar
25. Lee JA, Kim NH, Kim SJ, Choi EH, Lee HJ. Rapid identification of human adenovirus types 3 and 7 from respiratory specimens via multiplex type-specific PCR. J. Clin. Microbiol. 43(11), 5509–5514 (2005).
Medline CASGoogle Scholar
26. Schreiner S, Martinez R, Groitl P et al. Transcriptional activation of the adenoviral genome is mediated by capsid protein VI. PLoS Pathog. 8(2), e1002549 (2012).
Medline CASGoogle Scholar
27. Marrugal-Lorenzo JA, Serna-Gallego A, González-González L et al. Inhibition of adenovirus infection by mifepristone. Antivir. Res. 159, 77–83 (2018).
Medline CASGoogle Scholar
28. Pushpakom S, Iorio F, Eyers PA et al. Drug repurposing: progress, challenges and recommendations. Nat. Rev. Drug Discov. 18(1), 41–58 (2019).
Medline CASGoogle Scholar
29. Ali-Seyed M, Jantan I, Vijayaraghavan K, Bukhari SNA. Betulinic acid: recent advances in chemical modifications, effective delivery, and molecular mechanisms of a promising anticancer therapy. Chem. Biol. Drug Des. 87(4), 517–536 (2016).
Medline CASGoogle Scholar
30. Baltina L. Chemical modification of glycyrrhizic acid as a route to new bioactive compounds for medicine. Curr. Med. Chem. 10(2), 155–171 (2003).
Medline CASGoogle Scholar

Vol. 18, No. 5

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History

Received 3 January 2023

Accepted 20 April 2023

Published online 12 May 2023

Published in print April 2023

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Supplementary data

To view the supplementary data that accompany this paper please visit the journal website at: www.futuremedicine.com/doi/suppl/10.2217/fvl-2023-0001

Author contributions

C Li and L Qi designed the study. C Li, J Deng and L Qi performed the experiments, analyzed the data and made the figures. C Li drafted the manuscript and all authors reviewed and edited the manuscript.

Financial & competing interests disclosure

This work was supported by the Hospital-level project of Shenzhen Children's Hospital (ynkt2020-zz14) and the Shenzhen Fund for Guangdong Provincial High-level Clinical Key Specialties (SZGSP012). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

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Kuwanon X from mulberry leaves exhibits antiviral activity against human adenoviruses

Abstract

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