Lineages and sublineages of human papillomavirus type 16 in cervical samples of Iranian women
Abstract
Aim: This study was designed to analyze intratypic variations of human papillomavirus type 16 (HPV16) and to assess the risk of these variants for progression to cervical cancer. Materials & Methods: HPV16 variants of 58 women were determined by PCR-directed sequencing. Results: The most frequent lineage was D (67.2%) followed by A (32.8%). Lineage A was found predominantly in normal (62.5%) and cervical intraepithelial neoplasia-1 (CIN-1) (83.3%), while lineage D was the most prevalent variant in cervical cancer (100%). Conclusion: The present study revealed a distinct pattern of HPV16 variants in Iran. Based on our data, the predominant HPV16 lineage was D and there was a significant association between lineage D variants and cervical cancer.
Tweetable abstract
The intratypic variations of HPV16 were assessed in Iranian women with cervical cancer. HPV16 variants of 58 women were determined using PCR followed by directed sequencing. The most frequent lineage was D followed by A. This study revealed a distinct pattern of HPV16 variants in Iran.
Papers of special note have been highlighted as: • of interest; •• of considerable interest
References
- 1. GLOBOCAN. Estimated Cancer Incidence, Mortality and Prevalence Worldwide in 2020 (2020).
- 2. . Human papillomavirus (HPV) 18 genetic variants and cervical cancer risk in Taizhou area, China. Gene 647, 192–197 (2018).
- 3. . Cervical cancer. Best practice & research Clinical obstetrics & gynaecology. 26(3), 293–309 (2012).
- 4. Genotype distribution of Human Papillomavirus (HPV) in patients with Recurrent Respiratory Papillomatosis (RRP) in Iran. Respir. Med. 132, 270 (2017).
- 5. Human Papillomavirus Investigation in Head and Neck Squamous Cell Carcinoma: Initial Report from the Low Risk HPV Types Associations. Asian Pacific journal of cancer prevention. 18(9), 2573 (2017).
- 6. The biology and life-cycle of human papillomaviruses. Vaccine 30, F55–F70 (2012).
- 7. . The biology of human papillomaviruses. Human Papillomavirus. 45, 19–32 (2014).
- 8. Human papillomavirus types in 115,789 HPV-positive women: a meta-analysis from cervical infection to cancer. Int. J. Cancer 131(10), 2349–2359 (2012).
- 9. . Human papillomavirus burden in different cancers in Iran: a systematic assessment. Asian Pac J Cancer Prev. 15(17), 7029–7035 (2014).
- 10. . Fundamental Biology of Human Papillomaviruses. Recurrent Respiratory Papillomatosis Springer, Cham (2018).1–17
- 11. . Human papillomavirus in cervical cancer and oropharyngeal cancer: one cause, two diseases. Cancer 123(12), 2219–2229 (2017).
- 12. Risks for persistence and progression by human papillomavirus type 16 variant lineages among a population-based sample of Danish women. Cancer Epidemiology and Prevention Biomarkers. 20(7), 1315–1321 (2011).
- 13. Predictors of human papillomavirus persistence among women with equivocal or mildly abnormal cytology. Int. J. Cancer 126(3), 684–691 (2010).
- 14. Classification and evolution of human papillomavirus genome variants: alpha-5 (HPV26, 51, 69, 82), Alpha-6 (HPV30, 53, 56, 66), Alpha-11 (HPV34, 73), Alpha-13 (HPV54) and Alpha-3 (HPV61). Virology 516, 86–101 (2018).
- 15. . Human papillomavirus genome variants. Virology 445(1–2), 232–243 (2013). •• The comperehensive classification of lineages and sulineages of several HPV genotypes.
- 16. Human papillomavirus type 16 variant lineages in United States populations characterized by nucleotide sequence analysis of the E6, L2, and L1 coding segments. J. Virol. 69(12), 7743–7753 (1995).
- 17. Human papillomavirus type 16 sequence variation in cervical cancers: a worldwide perspective. J. Virol. 71(3), 2463–2472 (1997).
- 18. E6 molecular variants of human papillomavirus (HPV) type 16: an updated and unified criterion for clustering and nomenclature. Virology 410(1), 201–215 (2011).
- 19. Human papillomavirus 16 sub-lineage dispersal and cervical cancer risk worldwide: whole viral genome sequences from 7116 HPV16-positive women. Papillomavirus Res. 7, 67–74 (2019). •• A comperehensive study regard to HPV16 lineages and sublineages in the world.
- 20. . HPV 16 and its variants: minor genome variations make a big difference. J. Natl Cancer Inst. 108(9), djw123 (2016).
- 21. Identification of human papillomavirus-16 E6 variation in cervical cancer and their impact on T and B cell epitopes. J. Virol. Methods 218, 51–58 (2015).
- 22. Complete genome sequences of eight human papillomavirus type 16 Asian American and European variant isolates from cervical biopsies and lesions in Indian women. Genome announcements. 4(3), e00243–16 (2016).
- 23. Differential HPV16 variant distribution in squamous cell carcinoma, adenocarcinoma and adenosquamous cell carcinoma. Int. J. Cancer 140(9), 2092–2100 (2017).
- 24. Epidemiology of cervical cancer and human papilloma virus infection among Iranian women – analyses of national data and systematic review of the literature. Gynecol. Oncol. 128(2), 277–281 (2013).
- 25. Meta-analysis of type-specific human papillomavirus prevalence in Iranian women with normal cytology, precancerous cervical lesions and invasive cervical cancer: implications for screening and vaccination. J. Med. Virol. 87(2), 287–295 (2015).
- 26. Human papillomavirus type 16 lineage analysis based on E6 region in cervical samples of Iranian women. Infect Genet Evol. 55, 26–30 (2017). • First report of HPV16 lineages and sublineages in Iran.
- 27. Genetic Diversity of HPV 16 and HPV 18 Based on Partial Long Control Region in Iranian Women. Canadian Journal of Infectious Diseases and Medical Microbiology. 2022, 4759871 (2022).
- 28. . Commercially available assays for multiplex detection of alpha human papillomaviruses. Expert review of anti-infective therapy. 8(10), 1139–1162 (2010).
- 29. Human papillomavirus type 16 variant analysis of E6, E7, and L1 [corrected] genes and long control region in [corrected] cervical carcinomas in patients in northeast China. J. Clin. Microbiol. 49(7), 2656–2663 (2011).
- 30. Characterization of sequence variations within HPV16 isolates among Indian women: prediction of causal role of rare non-synonymous variations within intact isolates in cervical cancer pathogenesis. Virology 377(1), 143–150 (2008).
- 31. Genetic diversity of HPV 16 and HPV 18 based on partial long control region in Iranian women. Can J Infect Dis Med Microbiol. 2022, 4759871 (2022). • A new study regard to HPV16 lineages and sublineages in Iran.
- 32. HPV16 genetic variation and the development of cervical cancer worldwide. Br. J. Cancer 108(1), 240 (2013).
- 33. Human papillomavirus type 16 genetic variants: phylogeny and classification based on E6 and LCR. J. Virol. 86(12), 6855–6861 (2012).
- 34. Human papillomavirus type 16 and 18 variants: race-related distribution and persistence. J. Natl Cancer Inst. 98(15), 1045–1052 (2006).
- 35. Enhanced oncogenicity of human papillomavirus type 16 (HPV16) variants in Japanese population. Cancer Lett. 156(2), 159–165 (2000).
- 36. Asian-American variants of human papillomavirus 16 and risk for cervical cancer: a case-control study. J. Natl Cancer Inst. 93(17), 1325–1330 (2001).
- 37. Distribution of HPV16 and 18 intratypic variants in normal cytology, intraepithelial lesions, and cervical cancer in a Mexican population. Gynecol. Oncol. 102(2), 230–235 (2006).
- 38. Analysis of human papillomavirus type-16 variants in Italian women with cervical intraepithelial neoplasia and cervical cancer. J. Med. Virol. 74(1), 117–126 (2004).
- 39. Human papillomavirus 16 non-European variants are preferentially associated with high-grade cervical lesions. PLOS ONE. 9(7), e100746 (2014).
- 40. Human papillomavirus type 16 variants and risk of cervical cancer. J NatlCancer Inst. 93(4), 315–318 (2001).
- 41. A population-based prospective study of carcinogenic human papillomavirus variant lineages, viral persistence, and cervical neoplasia. Cancer Res. 70(8), 3159–3169 (2010).
- 42. High grade cervical lesions are caused preferentially by non-European variants of HPVs 16 and 18. Int. J. Cancer 120(8), 1763–1768 (2007).
- 43. Association of HPV16 E6 variants with diagnostic severity in cervical cytology samples of 354 women in a US population. Int. J. Cancer 125(11), 2609–2613 (2009).
- 44. . Human papillomavirus type 16/18 oncoproteins: potential therapeutic targets in non-smoking associated lung cancer. Asian Pacific Journal of Cancer Prevention. 13(11), 5363–5369 (2012).
- 45. . Papillomavirus E6 oncoproteins. Virology 445(1–2), 115–137 (2013).
- 46. HPV16 E6 variants: frequency, association with HPV types and in silico analysis of the identified novel variants. J. Med. Virol. 86(6), 968–974 (2014).
- 47. Molecular variants of HPV-16 associated with cervical cancer in Indian population. Int. J. Cancer 125(1), 91–103 (2009).
- 48. Human papillomavirus 16 E6 variants differ in their dysregulation of human keratinocyte differentiation and apoptosis. Virology 383(1), 69–77 (2009).
- 49. . Transmission between archaic and modern human ancestors during the evolution of the oncogenic human papillomavirus 16. Mol. Biol. Evol. 34(1), 4–19 (2017).