We use cookies to improve your experience. By continuing to browse this site, you accept our cookie policy.×
Skip main navigation
Aging Health
Bioelectronics in Medicine
Biomarkers in Medicine
Breast Cancer Management
CNS Oncology
Colorectal Cancer
Concussion
Epigenomics
Future Cardiology
Future Medicine AI
Future Microbiology
Future Neurology
Future Oncology
Future Rare Diseases
Future Virology
Hepatic Oncology
HIV Therapy
Immunotherapy
International Journal of Endocrine Oncology
International Journal of Hematologic Oncology
Journal of 3D Printing in Medicine
Lung Cancer Management
Melanoma Management
Nanomedicine
Neurodegenerative Disease Management
Pain Management
Pediatric Health
Personalized Medicine
Pharmacogenomics
Regenerative Medicine

Loop-mediated isothermal amplification assay in combination with lateral flow dipstick in HSV type 1

    Fei Gao‡

    Clinical Research Center, HE Eye Hospital of He University, Shenyang, Liaoning, China

    College of Life Science & Health, Northeastern University, Shenyang, Liaoning, China

    ‡Authors contributed equally

    Search for more papers by this author

    ,
    Zhuoshi Wang‡

    Stem Cell Research Center, Precision Medical Innovation Institute, He University, Shenyang, Liaoning, China

    ‡Authors contributed equally

    Search for more papers by this author

    ,
    Mingqi Zhang

    Stem Cell Research Center, Precision Medical Innovation Institute, He University, Shenyang, Liaoning, China

    ,
    Yan Zhao

    Stem Cell Research Center, Precision Medical Innovation Institute, He University, Shenyang, Liaoning, China

    ,
    Tao Yao

    Clinical Research Center, HE Eye Hospital of He University, Shenyang, Liaoning, China

    ,
    Ling Xu

    Clinical Research Center, HE Eye Hospital of He University, Shenyang, Liaoning, China

    ,
    Xiangdong He

    Stem Cell Research Center, Precision Medical Innovation Institute, He University, Shenyang, Liaoning, China

    ,
    Bing Wang

    **Author for correspondence:

    E-mail Address: wangbing@mail.neu.edu.cn

    College of Life Science & Health, Northeastern University, Shenyang, Liaoning, China

    &
    Wei He

    *Author for correspondence: Tel.: +86 024 86502018;

    E-mail Address: hsyk2017@163.com

    Clinical Research Center, HE Eye Hospital of He University, Shenyang, Liaoning, China

    Stem Cell Research Center, Precision Medical Innovation Institute, He University, Shenyang, Liaoning, China

    Published Online:

    Aim: Diagnostic methods for HSV-1 need to be updated. Materials & methods: Serial dilutions of HSV-1 DNA and ocular pathogenic microorganisms were tested by loop-mediated isothermal amplification-lateral flow dipstick assay (LAMP-LFD assay). The reliability of the LAMP-LFD assay and quantitative real-time PCR (qPCR) for HSV-1 detection was also validated using 30 clinical swab samples. Results: The LAMP reaction is optimal at 65°C and 30 min. And the LAMP-LFD assay could detect HSV-1 up to 10 copies/μl and no cross-reactivity with other pathogens was observed. Moreover, LAMP-LFD had similar positive rates to qPCR in clinical samples, and sensitivity and specificity were highly consistent with those of qPCR. Conclusion: LAMP-LFD assay is a rapid technique for the diagnosis of HSV-1.

    Papers of special note have been highlighted as: • of interest

    References

    • 1. Zhang X, Jiang Q, Xu X et al. Immune mechanisms induced by an HSV-1 mutant strain: discrepancy analysis of the immune system gene profile in comparison with a wild-type strain. Vaccine 36(18), 2394–2402 (2018).
    • 2. Tsatsos M, MacGregor C, Athanasiadis I et al. Herpes simplex virus keratitis: an update of the pathogenesis and current treatment with oral and topical antiviral agents. Clin. Exp. Ophthalmol. 44(9), 824–837 (2016).
    • 3. Koujah L, Suryawanshi RK, Shukla D. Pathological processes activated by herpes simplex virus-1 (HSV-1) infection in the cornea. Cell. Mol. Life Sci. 76(3), 405–419 (2019).
    • 4. Farooq AV, Shukla D. Herpes simplex epithelial and stromal keratitis: an epidemiologic update. Surv. Ophthalmol. 57(5), 448–462 (2012).
    • 5. Azher TN, Yin XT, Tajfirouz D, Huang AJ, Stuart PM. Herpes simplex keratitis: challenges in diagnosis and clinical management. Clin. Ophthalmol. 11, 185–191 (2017).
    • 6. Anderson NW, Buchan BW, Ledeboer NA. Light microscopy, culture, molecular, and serologic methods for detection of herpes simplex virus. J. Clin. Microbiol. 52(1), 2–8 (2014).
    • 7. Faron ML, Ledeboer NA, Patel A et al. Multicenter evaluation of Meridian Bioscience HSV 1&2 Molecular Assay for Detection of Herpes Simplex Virus 1 and 2 from clinical cutaneous and mucocutaneous specimens. J. Clin. Microbiol. 54(8), 2008–2013 (2016).
    • 8. Pandori MW, Lei J, Wong EH, Klausner J, Liska S. Real-time PCR for detection of herpes simplex virus without nucleic acid extraction. BMC Infect. Dis. 6, 104 (2006).
    • 9. Lalle M, Possenti A, Dubey JP, Pozio E. Loop-mediated isothermal amplification-lateral-flow dipstick (LAMP-LFD) to detect Toxoplasma gondii oocyst in ready-to-eat salad. Food Microbiol. 70, 137–142 (2018).
    • 10. Kaewphinit T, Arunrut N, Kiatpathomchai W et al. Detection of Mycobacterium tuberculosis by using loop-mediated isothermal amplification combined with a lateral flow dipstick in clinical samples. Biomed. Res. Int. 2013, 926230 (2013).
    • 11. Parida M, Sannarangaiah S, Dash PK, Rao PVL, Morita K. Loop mediated isothermal amplification (LAMP): a new generation of innovative gene amplification technique; perspectives in clinical diagnosis of infectious diseases. Rev. Med. Virol. 18(6), 407–421 (2010).
    • 12. Jawla J, Kumar RR, Mendiratta SK et al. Paper-based loop-mediated isothermal amplification and lateral flow (LAMP-LF) assay for identification of tissues of cattle origin. Anal Chim. Acta. 1150, 338220 (2021).
    • 13. Zhang J, Cao J, Zhu M, Xu M, Shi F. Loop-mediated isothermal amplification-lateral-flow dipstick (LAMP-LFD) to detect Mycoplasma ovipneumoniae. World J. Microbiol. Biotechnol. 35(2), 31 (2019).
    • 14. Wastling SL, Picozzi K, Kakembo AS, Welburn SC. LAMP for human African trypanosomiasis: a comparative study of detection formats. PLoS Negl. Trop. Dis. 4(11), e865 (2010).
    • 15. Quyen TL, Ngo TA, Bang DD, Madsen M, Wolff A. Classification of multiple DNA dyes based on inhibition effects on real-time loop-mediated isothermal amplification (LAMP): prospect for point of care setting. Front. Microbiol. 10, 2234 (2019).
    • 16. Sadatsafavi M, Najafzadeh M, Lynd L, Marra C. Reliability studies of diagnostic tests are not using enough observers for robust estimation of interobserver agreement: a simulation study. J. Clin. Epidemiol. 61(7), 722–727 (2008).
    • 17. Dehghan Esmatabadi MJ, Bozorgmehr A, Motalebzadeh H et al. Techniques for evaluation of lamp amplicons and their applications in molecular biology. Asian Pac. J. Cancer Prev. 16(17), 7409–7414 (2015).
    • 18. Allgower SM, Hartmann CA, Lipinski C et al. LAMP-LFD based on isothermal amplification of multicopy gene ORF160b: applicability for highly sensitive low-tech screening of allergenic soybean (Glycine max) in food. Foods 9(12), 1741 (2020). • Because wheat leaf rust caused by wheat rust has an important impact on global wheat production. They report on specific molecular assays for early detection of leaf rust of wheat pathogens by conventional PCR, qPCRand loop-mediated DNA isothermal amplification (LAMP). The developed assay facilitates early and rapid detection of the pathogen on wheat leaves with high sensitivity. The developed LAMP assay also helps in monitoring the pathogen, which will help in accurate prediction and precise control of this disease.
    • 19. Kumvongpin R, Jearanaikoon P, Wilailuckana C et al. Detection assay for HPV16 and HPV18 by loop-mediated isothermal amplifcation with lateral flow dipstick tests. Mol. Med. Rep. 15(5), 3203–3209 (2017).
    • 20. Zhang X, Lowe SB, Gooding JJ. Brief review of monitoring methods for loop-mediated isothermal amplification (LAMP). Biosens. Bioelectron. 61, 491–499 (2014).
    • 21. Badoul NA, Kagira J, Ng'ong'a F, Dinka H. Development of loop-mediated isothermal amplification combined with lateral flow dipstick assay for a rapid and sensitive detection of cystic echinococcosis in livestock in Kenya. J. Trop. Med. 2022, 4928009 (2022).
    • 22. Joon D, Nimesh M, Gupta S et al. Development and evaluation of rapid and specific sdaA LAMP-LFD assay with Xpert MTB/RIF assay for diagnosis of tuberculosis. J. Microbiol. Methods 159, 161–166 (2019).
    • 23. Allgöwer SM, Hartmann CA, Holzhauser T. The development of highly specific and sensitive primers for the detection of potentially allergenic soybean (Glycine max) using loop-mediated isothermal amplification combined with lateral flow dipstick (LAMP-LFD). Foods 9(4), 423 (2020).
    • 24. Wang L, Chen G, Zhang C, Wang Y, Sun R. Application of loop-mediated isothermal amplification combined with lateral flow dipstick to rapid and sensitive detection of Alexandrium catenella. Environ. Sci. Pollut. Res. Int. 27(4), 4246–4257 (2020).
    • 25. Notomi T, Mori Y, Tomita N, Kanda H. Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects. J. Microbiol. 53(1), 1–5 (2015).
    • 26. Lipsitch M, Davis G, Corey L. Potential benefits of a serodiagnostic test for herpes simplex virus type 1 (HSV-1) to prevent neonatal HSV-1 infection. Sex. Transm. Dis. 29(7), 399–405 (2002).
    • 27. Rechenchoski DZ, Faccin-Galhardi LC, Linhares REC, Nozawa C. Herpesvirus: an underestimated virus. Folia Microbiol. (Praha) 62(2), 151–156 (2017).
    • 28. Fraser NW, Valyi-Nagy T. Viral, neuronal and immune factors which may influence herpes simplex virus (HSV) latency and reactivation. Microb. Pathog. 15(2), 83 (1993).
    • 29. Enomoto Y, Yoshikawa T, Ihira M, Akimoto S, Asano Y. Rapid diagnosis of herpes simplex virus infection by a loop-mediated isothermal amplification method. J. Clin. Microbiol. 43(2), 951–955 (2005).
    • 30. Kaneko H, Iida T, Aoki K, Ohno S, Suzutani T. Sensitive and rapid detection of herpes simplex virus and varicella-zoster virus DNA by loop-mediated isothermal amplification. J. Clin. Microbiol. 43(7), 3290 (2005).
    • 31. Shin J, Ra H, Rho CR. Herpes simplex virus linear endotheliitis in a post-keratoplasty patient: a case report. Medicine 98(3), e14191 (2019).
    • 32. Reyes P, Heidelberger C. Fluorinated pyrimidines XXVI. Mammalian thymidylate synthetase: its mechanism of action and inhibition by fluorinated nucleotides. Mol. Pharmacol. 1(1), 14–30 (1965).
    • 33. Arshad Z, Alturkistani A, Brindley D et al. Tools for the diagnosis of herpes simplex virus 1/2: Systematic Review of studies published between 2012 and 2018. JMIR Public Health Surveill. 5(2), e14216 (2019).
    • 34. Manjunatha C, Sharma S, Kulshreshtha D et al. Rapid detection of Puccinia triticina causing leaf rust of wheat by PCR and loop mediated isothermal amplification. PLOS ONE 13(4), e0196409 (2018).
    • 35. Reddy AK, Balne PK, Reddy RK, Mathai A, Kaur I. Loop-mediated isothermal amplification assay for the diagnosis of retinitis caused by herpes simplex virus-1. Clin. Microbiol. Infect. 17(2), 210–213 (2011).
    • 36. Hardinge P, Kiddle G, Tisi L, Murray JAH. Optimised LAMP allows single copy detection of 35Sp and NOSt in transgenic maize using Bioluminescent Assay in Real Time (BART). Sci. Rep. 8(1), 17590 (2018).
    • 37. Vergara A, Vendrell R, Alejo-Cancho I et al. Rapid detection of herpes simplex virus-1 from bronchoalveolar lavage fluids using loop-mediated isothermal amplification (LAMP). Enferm. Infecc. Microbiol. Clin. (Engl. Ed.) 37(5), 353–354 (2019).
    • 38. Waters RA, Fowler VL, Armson B et al. Preliminary validation of direct detection of foot-and-mouth disease virus within clinical samples using reverse transcription loop-mediated isothermal amplification coupled with a simple lateral flow device for detection. PLOS ONE 9(8), e105630 (2014).