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Evaluation of CCR5-Δ32 mutation and HIV-1 surveillance drug-resistance mutations in peripheral blood mononuclear cells of long-term non progressors of HIV-1-infected individuals

    Khadijeh Khanaliha

    Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran

    ,
    Farah Bokharaei-Salim

    *Author for correspondence: Tel.: +98 21 6604 7831;

    E-mail Address: bokharaei.f@iums.ac.ir

    Department of Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

    ,
    Tahereh Donyavi

    Medical Biotechnology Department, School of Allied Medical Sciences, Iran University of Medical Sciences

    ,
    Javid Sadri Nahand

    Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    ,
    Arezoo Marjani

    Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

    ,
    Sogol Jamshidi

    Department of Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

    ,
    Alireza Khatami

    Department of Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

    ,
    Maryam Moghaddas

    School of Medicine, Iran University of Medical Sciences, Tehran, Iran

    ,
    Maryam Esghaei

    Department of Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

    &
    Atousa Fakhim

    Department of Architectural Engineering, Faculty of Engineering, Islamic Azad University, South Tehran Branch, Tehran, Iran

    Published Online:https://doi.org/10.2217/fvl-2021-0028

    Aim: This study aimed to evaluate chemokine receptor 5 delta 32 (CCR5-Δ32) mutation and HIV-1 surveillance drug-resistance mutations (SDRMs) in peripheral blood mononuclear cells of long-term non progressors (LTNPs) of HIV-1-infected individuals. Materials & methods: This research was performed on 197 treatment-naive HIV-1-infected patients. After follow-up, it was determined that 15 (7.6%) of these people were LTNPs. The PCR assay was performed to identify the CCR5 genotype and HIV-1 SDRMs. Results: One (6.7%) of the LTNPs was heterozygous (wt/Δ32) for the CCR5 delta 32 (CCR5Δ32). However, none of the individuals was homozygous for this mutation (Δ32/Δ32). Moreover, none of the LTNPs showed HIV-1 SDRMs. The CRF35-AD subtype was the most dominant subtype, with a percentage of 93.3%. Conclusion: Iranian elite controllers are negative for CCR5-delta 32 homozygous genotype and drug resistance against antiretroviral drugs.

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