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Identification of adenovirus species in Iranian pediatric population with severe acute respiratory infections

Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

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Jila Yavarian

Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

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Somayeh Shatizadeh Malekshahi

Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

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Maryam Naseri

Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

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Azadeh Shadab

Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

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Nastaran Ghavami

Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

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Talat Mokhtari-Azad

*Author for correspondence: Tel.: +98 21 88962343; Fax: +98 21 88962343;

E-mail Address: mokhtari@tums.ac.ir

Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

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Published Online:7 Nov 2019https://doi.org/10.2217/fvl-2019-0074

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Abstract

Aim: In this study we aimed to find the rate of human adenovirus (HAdV) infections and identify HAdVs molecular epidemiology in children younger than 5 years old with clinical diagnosis of severe acute respiratory infections in Iran. Patients & methods: A semi-nested PCR with in-house designed primers for HAdV was performed on 200 oropharyngeal swabs collected within 5 days from the onset of symptoms. The positive samples were subjected to sequencing and a phylogenetic tree was drawn. Results: Out of 200 specimens, 71 (35.5%) cases were positive for HAdVs. Detected strains matched with HAdV species B (80.3%), C (15.5%), D (2.8%) and E (1.8%). HAdV- B14/55 and HAdV-B3 were the most prevalent types, respectively. Conclusion: This study showed that infections with HAdV species B, were common in children, who were clinically diagnosed as severe acute respiratory infection cases in Iran. The results could be useful for future epidemiological researches.

Keywords:

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Vol. 14, No. 9

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History

Received 30 June 2019

Accepted 19 September 2019

Published online 7 November 2019

Published in print September 2019

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Acknowledgments

We express our thanks to our colleagues at Iran National Influenza Center, School of Public Health, Tehran University of Medical Sciences.

Financial & competing interests disclosure

This study was funded and supported by Tehran University of Medical Sciences (Grant No. 91-04-27-21030). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

All specimens included in this study were collected by Iran Ministry of Health for national influenza surveillance. No extra samples were collected for this study. The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations.

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