Short Communication

Bioinformatic analysis of miRNA–mRNA interaction associated with LMP2A gene in nasopharyngeal carcinoma

Department of Pathogeny Biology, Qingdao University Medical College, 38 Dengzhou Road, Shandong, 266021, China

Department of Clinical Laboratory, the Affiliated Hospital of Qingdao University, 59 Haier Road, Shandong, 266003, China

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Yang Yang

Department of Pathogeny Biology, Qingdao University Medical College, 38 Dengzhou Road, Shandong, 266021, China

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Shuzhen Liu

Department of Blood Transfusion, the Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Shandong, 266003, China

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Jiayi Wang

Department of Pathogeny Biology, Qingdao University Medical College, 38 Dengzhou Road, Shandong, 266021, China

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Bing Luo

*Author for correspondence: Tel.: +86 532 8299 1083;

E-mail Address: qdluobing@163.com

Department of Pathogeny Biology, Qingdao University Medical College, 38 Dengzhou Road, Shandong, 266021, China

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Published Online:8 Jul 2019https://doi.org/10.2217/fvl-2018-0167

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Abstract

Aim: The purpose of the study was to construct miRNA–mRNA network associated with LMP2A in nasopharyngeal carcinoma (NPC). Materials & methods: The dataset GSE53914, GSE12452 and GSE26596 were downloaded from Gene Expression Omnibus and differentially expressed genes (DEGs) identified by GEO2R. Gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were performed by ClusterProfiler R package. Protein–protein interaction network and mRNA–miRNA network associated with LMP2A were constructed. Hub genes were identified by Cytoscape. Results: The 135 DEGs associated with LMP2A were identified in NPC. Gene ontology function analysis showed DEGs were significantly enriched in cell–cell adhesion and NF-κB pathway. The hub genes were related to cell cycle. miRNA–mRNA network associated with LMP2A was constructed. Conclusion: The network may provide a way to explore the function of LMP2A in NPC by miRNA.

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Papers of special note have been highlighted as: • of interest; •• of considerable interest

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Vol. 14, No. 6

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History

Received 3 October 2018

Accepted 5 May 2019

Published online 8 July 2019

Published in print June 2019

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Keywords

Acknowledgments

This research was supported by the technical assistance of the online analysis platform Gene-Cloud of Biotechnology Information website.

Author contributions

Bing Luo and Qianzhu Shi contributed to the conception of the project. Yu Ding and Shuzhen Liu discussed and offered advice for the study. Yang Yang and Jiayi Wang helped analyze the data. Qianzhu Shi wrote the manuscript.

Financial and competing interests disclosure

This work was supported by National Natural Science Foundation of China (NSFC 81571995); Natural Science Foundation of Shandong Province (ZR2015HM069, ZR2017BH106). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The studies have been approved by the Medical Ethics Committee at the Medical College of Qingdao University, China and have been performed in accordance with the ethical standards as laid down in the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards.

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