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Research ArticleOpen Accesscc iconby iconnc iconnd icon

Improvement strategies for successful next-generation sequencing analysis of lung cancer

    Kei Kunimasa

    *Author for correspondence:

    E-mail Address: kei.kunimasa@oici.jp

    Department of Thoracic Oncology, Osaka International Cancer Institute, Osaka, 541-8567, Japan

    ,
    Shingo Matsumoto

    Department of Thoracic Oncology, National Cancer Center Hospital East, Kashiwa, 277-8577, Japan

    ,
    Kazumi Nishino

    Department of Thoracic Oncology, Osaka International Cancer Institute, Osaka, 541-8567, Japan

    ,
    Harumi Nakamura

    Department of Diagnostic Pathology & Cytology, Osaka International Cancer Institute, Osaka, 541-8567, Japan

    ,
    Hanako Kuhara

    Department of Thoracic Oncology, Osaka International Cancer Institute, Osaka, 541-8567, Japan

    ,
    Motohiro Tamiya

    Department of Thoracic Oncology, Osaka International Cancer Institute, Osaka, 541-8567, Japan

    ,
    Takako Inoue

    Department of Thoracic Oncology, Osaka International Cancer Institute, Osaka, 541-8567, Japan

    ,
    Takahisa Kawamura

    Department of Thoracic Oncology, Osaka International Cancer Institute, Osaka, 541-8567, Japan

    ,
    Hayato Kawachi

    Department of Thoracic Oncology, Osaka International Cancer Institute, Osaka, 541-8567, Japan

    ,
    Kika Kuno

    Department of Thoracic Oncology, Osaka International Cancer Institute, Osaka, 541-8567, Japan

    ,
    Toru Kimura

    Department of General Thoracic Surgery, Osaka International Cancer Institute, Osaka, 541-8567, Japan

    ,
    Tomohiro Maniwa

    Department of General Thoracic Surgery, Osaka International Cancer Institute, Osaka, 541-8567, Japan

    ,
    Jiro Okami

    Department of General Thoracic Surgery, Osaka International Cancer Institute, Osaka, 541-8567, Japan

    ,
    Shin-ichi Nakatsuka

    Department of Diagnostic Pathology & Cytology, Osaka International Cancer Institute, Osaka, 541-8567, Japan

    ,
    Koichi Goto

    Department of Thoracic Oncology, National Cancer Center Hospital East, Kashiwa, 277-8577, Japan

    &
    Toru Kumagai

    Department of Thoracic Oncology, Osaka International Cancer Institute, Osaka, 541-8567, Japan

    Published Online:https://doi.org/10.2217/fon-2020-0332

    Aim: We aimed to improve the success rate of NGS (next-generation sequencing) analysis through improved strategies of lung cancer sampling. Materials & methods: The improvement strategies are as follows. Surgically resected specimens were preferentially submitted in cooperation with pathologists and surgeons. In bronchoscopic samples, the size of the sample collection device and the number of samples collected was increased. Results: The strategies increased the success rate of NGS analysis of DNA from 69.3 to 91.1%, and that of RNA from 64.6 to 90.0%. Discussion: The introduction of strategies aimed at improving the success of NGS analysis resulted in an improvement in the success rate and brought us closer to the delivery of effective precision medicine in cancer therapy.

    Papers of special note have been highlighted as: • of interest; •• of considerable interest

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