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Sunitinib inhibits PD-L1 expression in osteosarcoma by targeting STAT3 and remodels the immune system in tumor-bearing mice

    Xian Liang Duan

    The Second Department of Orthopedic, The Affiliated Hospital of Beihua University, Jilin 132011, PR China

    ,
    Jian Ping Guo

    The Second Department of Orthopedic, The Affiliated Hospital of Beihua University, Jilin 132011, PR China

    ,
    Fan Li

    The Second Department of Orthopedic, The Affiliated Hospital of Beihua University, Jilin 132011, PR China

    ,
    Chao Xiu

    Medical Imaging Center, The Affiliated Hospital of Beihua University, Jilin 132011, PR China.

    &
    Hua Wang

    *Author for correspondence: Fax: 0432 6216 6003;

    E-mail Address: 981448258@qq.com

    The Department of Orthopedic, The Affiliated Hospital of Beihua University, Jilin 132011, PR China.

    Published Online:https://doi.org/10.2217/fon-2019-0725

    Aim: Exploring the mechanisms of the combination therapy using VEGFR-TKI and immune checkpoint inhibitors might be useful to control the development of osteosarcoma. Materials & methods: The expression of PD-L1 and STAT3 in osteosarcoma were determined with western blot. Proliferation, migration and invasion were determined with CCK-8 and Transwell assays. Lung metastases, tumor growth, survival and immune cell populations were performed in tumor-bearing mice. Results: Sunitinib reduced the expression of PD-L1 by inhibiting the activation of STAT3 and suppressed the migration and invasion in osteosarcoma cells. Combination therapy reduced lung metastases, tumor growth, improved survival and reverse tumor microenvironment in tumor-bearing mice. Conclusion: Sunitinib inhibits PD-L1 expression by targeting STAT3 and remodels the immune system in tumor-bearing mice.

    Papers of special note have been highlighted as: • of interest; •• of considerable interest

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