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Study of IsCT analogue peptide against Candida albicans and toxicity/teratogenicity in zebrafish embryos (Danio rerio)

    Mariana C Galeane‡

    Department of Clinical Analysis, School of Pharmaceutical Sciences, São Paulo State University-UNESP, Araraquara, SP, Brazil

    ‡Authors contributed equally

    Search for more papers by this author

    ,
    Paulo C Gomes‡

    Department of Clinical Analysis, School of Pharmaceutical Sciences, São Paulo State University-UNESP, Araraquara, SP, Brazil

    ‡Authors contributed equally

    Search for more papers by this author

    ,
    Junya L Singulani

    Department of Clinical Analysis, School of Pharmaceutical Sciences, São Paulo State University-UNESP, Araraquara, SP, Brazil

    ,
    Maria JS Mendes-Giannini

    Department of Clinical Analysis, School of Pharmaceutical Sciences, São Paulo State University-UNESP, Araraquara, SP, Brazil

    &
    Ana M Fusco-Almeida

    *Author for correspondence: Tel.: +55 (16) 3301 4640 4641;

    E-mail Address: ana.marisa@uol.com.br

    Department of Clinical Analysis, School of Pharmaceutical Sciences, São Paulo State University-UNESP, Araraquara, SP, Brazil

    Published Online:https://doi.org/10.2217/fmb-2022-0210

    Aim: An IsCT analogue peptide (PepM3) was designed based on structural studies of wasp mastoparans and tested against Candida albicans. Its effects on fungal cell membranes and toxicity were evaluated. Materials & methods: Antifungal activity was analyzed using a microdilution susceptibility test. Toxicity was assessed using human skin keratinocytes (HaCaT) and zebrafish embryos. Results: PepM3 demonstrated activity against C. albicans and a synergistic effect with amphotericin B. The peptide presented fungicidal action with damage to the fungal cell membrane, low toxicity in HaCat cells and was nonteratogenic in zebrafish embryos. Conclusion: Evaluating structural modifications is essential for the development of new agents with potential activity against fungal pathogens and for the reduction of toxic and teratogenic effects.

    Papers of special note have been highlighted as: • of interest

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