New compound probiotic beverage protects against antibiotic-associated diarrhea in mice by modulating the microbiota
Abstract
Probiotics and their products are the classic way to treat and prevent gastrointestinal diseases. In this study, the authors designed new combinations and doses of probiotic beverages for antibiotic-associated diarrhea. Group S1 was different from the other groups, including Lactobacillus rhamnosus HN001, Lactobacillus acidophilus NCFM and Bifidobacterium lactis BI-07. Its inulin content was higher than those of the other groups. Mice were induced with a 16-day administration of triple antibiotics in advance for 2 weeks prior to antibiotic treatment. In the experiment, the treatment group returned to normal more quickly than the placebo group. In group S1, the relative abundance of the phylum Firmicutes and genus Lactobacillus increased, and the structure of the microbiota was the closest to normal among all groups. In conclusion, the combinations of probiotic beverages effectively caused structural recovery of the gut and fecal microbiota against antibiotic-associated diarrhea, and the S1 formula showed the best efficacy.
Graphical abstract
References
- 1. . Toxin-producing Klebsiella oxytoca as a cause of antibiotic-associated colitis. Almanac Clinical Medicine 46(5), 497–503 (2018).
- 2. Expert consensus document. The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat. Rev. Gastroenterol. Hepatol. 11, 506–514 (2014).
- 3. . Strain-specificity and disease-specificity of probiotic efficacy: a systematic review and meta-analysis. Front. Med. 5, 124 (2018).
- 4. Probiotics for the prevention of antibiotic-associated diarrhoea in children. J. Pediatr. Gastr. Nutr. 12(6), 463–466 (2016).
- 5. . Prebiotic inulin enriched with oligofructose in combination with the probiotics Lactobacillus rhamnosus and Bifidobacterium lactis modulates intestinal immune functions in rats. J. Nutri. 134(1), 153–156 (2004).
- 6. . Probiotic strains and their combination inhibit in vitro adhesion of pathogens to pig intestinal mucosa. Curr. Microbiol. 55(3), 260–265 (2007).
- 7. Fermented wheat aleurone enriched with probiotic strains LGG and Bb12 modulates markers of tumor progression in human colon cells. Nutr. Cancer 63(1), 151–160 (2010).
- 8. Fermented milk supplemented with probiotics and prebiotics can effectively alter the intestinal microbiota and immunity of host animals. J. Dairy Sci. 95(9), 4813–4822 (2012).
- 9. . Probiotics for the prevention of pediatric antibiotic-associated diarrhoea: summary of a Cochrane review. Explore 15(5), 382–383 (2019).
- 10. Bacteroides fragilis protects against antibiotic-associated diarrhoea in rats by modulating intestinal defenses. Front. Immunol. 9, 1040 (2018).
- 11. Reclamation of herb residues using probiotics and their therapeutic effect on diarrhoea. Mediat. Inflamm. 2017, 1–8 (2017).
- 12. . Circular RNA expression profiles and features in NAFLD mice: a study using RNA-seq data. J. Translati. Med. 18(1), 476 (2020).
- 13. Acute elevated resistin exacerbates mitochondrial damage and aggravates liver steatosis through AMPK/PGC-1 α signaling pathway in male NAFLD mice. Horm. Metab. Res. 53, 132–144 (2021).
- 14. . Cutadapt removes adapter sequences from high-throughput sequencing reads. Embnet J. 17(1), 10–12 (2011).
- 15. . VSEARCH: a versatile open source tool for metagenomics. PEERJ 10(4), 1–22 (2016).
- 16. Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons. Genome Res. 21(3), 494–504 (2011).
- 17. . UPARSE: highly accurate OTU sequences from microbial amplicon reads. Nature Methods: Techniques for Life Scientists Chemists 10(10), 996–998 (2013).
- 18. R C.J. Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl. Environ. Microb. 73(16), 5261–5267 (2007).
- 19. The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Res. 41(D1), D590–D596 (2013).
- 20. Structural changes of gut microbiota during berberine-mediated prevention of obesity and insulin resistance in high-fat diet-fed rats. PLOS ONE 7(8), e42529 (2012).
- 21. . Practical innovations for high-throughput amplicon sequencing. Nature Methods 10(10), 999–1002 (2013).
- 22. Clostridium butyricum combined with Bifidobacterium infantis probiotic mixture restores fecal microbiota and attenuates systemic inflammation in mice with antibiotic-associated diarrhoea. Biomed. Res. Int. 2015, 1–9 (2015).
- 23. Structural modulation of gut microbiota during alleviation of antibiotic-associated diarrhoea with herbal formula. Int. J. Biol. Macromol. 105, 1622–1629 (2017).
- 24. Bacteroides fragilis protects against antibiotic-associated diarrhoea in rats by modulating intestinal defenses. Front. Immunol. 9, 1040 (2018).
- 25. . Host immune response to antibiotic perturbation of the microbiota. Commentaries 3(2), 100–103 (2010).
- 26. . Antibiotics and the developing infant gut microbiota and resistome. Curr. Opin. MIcrobioL. 27, 51–56 (2015).
- 27. . Influence of a probiotic mixture on antibiotic induced microbiota disturbances. World J. Gastroenterol. 20(33), 11878–11885 (2014).
- 28. Body mass index and sex affect diverse microbial niches within the gut. Front. Microbiol. 9, 1–12 (2018).
- 29. . Bacterial diversity in intestinal mucosa of antibiotic-associated diarrhoea mice. 3 Biotech. 9(12), 444 (2019).
- 30. . Proteobacteria: microbial signature of dysbiosis in gut microbiota. Trends Biotechnol. 33(9), 496–503 (2015).
- 31. Antibiotics can cause weight loss by impairing gut microbiota in mice and the potent benefits of lactobacilli. Bioscience, Biotechnology Biochemistry 84, 1–10 (2020).
- 32. . Antibiotics and the gut microbiota. J. Clin. Invest. 124, 4212–4218 (2014).
- 33. . Postnatal administration of Lactobacillus rhamnosus HN001 ameliorates perinatal broad‐spectrum antibiotic‐induced reduction in myelopoiesis and t cell activation in mouse pups. Mol. Nutr. Food Res. 62, 1–33 (2018).
- 34. Probiotic bacteria Lactobacillus acidophilus NCFM and Bifidobacterium lactis Bi-07 versus placebo for the symptoms of bloating in patients with functional bowel disorders: a double-blind study. J. Clin. Gastroenterol. 45(6), 518–525 (2011).
- 35. . Members of the Lactobacillus genus complex (LGC) as opportunistic pathogens: a review. Microorganisms 7(5), 126 (2019).
- 36. . Probiotic properties of Weissella strains isolated from human faeces. Anaerobe 18(1), 96–102 (2012).
- 37. . Strain-specificity and disease-specificity of probiotic efficacy: a systematic review and meta-analysis. Frontiers in Medicine 5, 124 (2018).
- 38. . Meta-analysis of the effects of Bifidobacterium preparations for the prevention and treatment of pediatric antibiotic-associated diarrhoea in China. Complement. Ther. Med. 33, 105–113 (2017).
- 39. Microbiota benefits after inulin and partially hydrolized guar gum supplementation: a randomized clinical trial in constipated women. Nutricion Hospitalaria 27(1), 123–129 (2012).
- 40. Conventional and sugar-free probiotic white chocolate: effect of inulin DP on various quality properties and viability of probiotics. J. Funct. Foods 43, 206–213 (2018).
- 41. Plant-derived prebiotics and its health benefits. New Look Phytomedicine 2019, 63–88 (2019).