Preliminary Communication

Characterization of carbapenem-resistant Escherichia coli and Klebsiella: a role for AmpC-producing isolates

Department of Microbiology & Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

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Ehsan Aryan

Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

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Masoud Youssefi

https://orcid.org/0000-0001-6028-3573

Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

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Kiarash Ghazvini

Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

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Zahra Meshkat

Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

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Yousef Amini

Infectious Disease & Tropical Medicine Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran

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Hadi Safdari

Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

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Mohammad Derakhshan

Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

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Hadi Farsiani

*Author for correspondence: Tel.: +98 513 802 2205;

E-mail Address: farsianih@mums.ac.ir

https://orcid.org/0000-0002-4738-0245

Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

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Published Online:27 Apr 2023https://doi.org/10.2217/fmb-2021-0211

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Abstract

Aim: This study aimed to investigate the role of AmpC enzymes in carbapenem resistance among AmpC/extended-spectrum β-lactamase (ESBL)-producing clinical isolates of Escherichia coli and Klebsiella spp. Methods: Fifty-six bacterial strains that were AmpC producers were examined. The antibiotic susceptibility test was performed by the disk diffusion and E-test. The prevalence of the plasmid carbapenemase was determined using PCR. Results: The resistance to meropenem in the AmpC⁺/ESBL⁺ group was 64%, higher than that reported for the AmpC⁻/ESBL⁺ group. Ten isolates of the carbapenem-resistant AmpC producers were negative for carbapenemase-encoding genes. Conclusion: Carbapenem resistance among AmpC-producing isolates with negative results for carbapenemase-encoding genes potentially demonstrates the role of AmpC enzymes among these isolates.

Keywords:

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Vol. 18, No. 4

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History

Received 9 August 2021

Accepted 8 December 2022

Published online 27 April 2023

Published in print March 2023

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Keywords

Author contributions

K Salimiyan Rizi and H Farsiani contributed to the concept and design of the study. E Aryan, M Youssefi, K Ghazvini and Z Meshkat contributed with reagents and materials. Data acquisition was done by H Safdari and M Derakhshan. Data analysis and interpretation of results were done by Y Amini, H Farsiani and K Salimiyan Rizi. The manuscript was drafted by K Salimiyan Rizi and H Farsiani. All authors have critically revised the manuscript and approved the final version.

Acknowledgments

The authors wish to thank the staff of the bacteriology laboratory of Imam Reza, Akbar and Doctor Sheikh Hospitals (Mashhad, Iran) for their technical assistance.

Financial & competing interests disclosure

This work was supported by the Vice Chancellor for Research of Mashhad University of Medical Sciences (Mashhad, Iran) (grant no. 950839). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations.

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