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Rescue of streptomycin activity by piperine in Mycobacterium tuberculosis

    Leonora L Calsavara

    Postgraduate Program in Bioscience & Physiopathology, State University of Maringá (UEM), Maringá, Paraná, Brazil

    Department of Clinical Analysis & Biomedicine, Laboratory of Medical Bacteriology, State University of Maringa, Parana, Brazil

    ,
    Laíse A Hegeto

    Postgraduate Program in Health Sciences, State University of Maringá (UEM), Maringá, Paraná, Brazil

    Department of Clinical Analysis & Biomedicine, Laboratory of Medical Bacteriology, State University of Maringa, Parana, Brazil

    ,
    Eloisa G Sampiron

    Postgraduate Program in Health Sciences, State University of Maringá (UEM), Maringá, Paraná, Brazil

    Department of Clinical Analysis & Biomedicine, Laboratory of Medical Bacteriology, State University of Maringa, Parana, Brazil

    ,
    Giovana F Costacurta

    Postgraduate Program in Health Sciences, State University of Maringá (UEM), Maringá, Paraná, Brazil

    Department of Clinical Analysis & Biomedicine, Laboratory of Medical Bacteriology, State University of Maringa, Parana, Brazil

    ,
    Letícia S Murase

    Postgraduate Program in Health Sciences, State University of Maringá (UEM), Maringá, Paraná, Brazil

    Department of Clinical Analysis & Biomedicine, Laboratory of Medical Bacteriology, State University of Maringa, Parana, Brazil

    ,
    João VP Souza

    Postgraduate Program in Bioscience & Physiopathology, State University of Maringá (UEM), Maringá, Paraná, Brazil

    Department of Clinical Analysis & Biomedicine, Laboratory of Medical Bacteriology, State University of Maringa, Parana, Brazil

    ,
    Aryadne L de Almeida

    Postgraduate Program in Bioscience & Physiopathology, State University of Maringá (UEM), Maringá, Paraná, Brazil

    Department of Clinical Analysis & Biomedicine, Laboratory of Medical Bacteriology, State University of Maringa, Parana, Brazil

    ,
    Nathally C de S Santos

    Postgraduate Program in Bioscience & Physiopathology, State University of Maringá (UEM), Maringá, Paraná, Brazil

    Department of Clinical Analysis & Biomedicine, Laboratory of Medical Bacteriology, State University of Maringa, Parana, Brazil

    ,
    Vera LD Siqueira

    Postgraduate Program in Bioscience & Physiopathology, State University of Maringá (UEM), Maringá, Paraná, Brazil

    Department of Clinical Analysis & Biomedicine, Laboratory of Medical Bacteriology, State University of Maringa, Parana, Brazil

    ,
    Regiane B de L Scodro

    Postgraduate Program in Health Sciences, State University of Maringá (UEM), Maringá, Paraná, Brazil

    Department of Clinical Analysis & Biomedicine, Laboratory of Medical Bacteriology, State University of Maringa, Parana, Brazil

    ,
    Rosilene F Cardoso

    Postgraduate Program in Bioscience & Physiopathology, State University of Maringá (UEM), Maringá, Paraná, Brazil

    Postgraduate Program in Health Sciences, State University of Maringá (UEM), Maringá, Paraná, Brazil

    Department of Clinical Analysis & Biomedicine, Laboratory of Medical Bacteriology, State University of Maringa, Parana, Brazil

    &
    Katiany R Caleffi-Ferracioli

    *Author for correspondence:

    E-mail Address: krcferracioli2@uem.br

    Postgraduate Program in Bioscience & Physiopathology, State University of Maringá (UEM), Maringá, Paraná, Brazil

    Department of Clinical Analysis & Biomedicine, Laboratory of Medical Bacteriology, State University of Maringa, Parana, Brazil

    Published Online:https://doi.org/10.2217/fmb-2020-0124

    Aim: To evaluate the modulatory effect of piperine (PIP) on streptomycin (SM) activity in Mycobacterium tuberculosis (Mtb). Materials & methods: SM and PIP minimum inhibitory concentration (MIC) and combinatory activity were determined in Mtb H37Rv and in susceptible and resistant clinical isolates. Ethidium bromide accumulation assay and relative quantification of efflux pumps genes (rv1258c, rv1218c and rv2942), after SM and SM+PIP combination exposure, were also performed. Results: PIP concentration of 25 μg/ml (1/4× MIC) was able to inhibit efflux pumps activity, to modulate SM activity in Mtb, and conducted changes in the relative quantification of efflux pumps genes. Conclusion: SM+PIP combination was able to rescue the SM-susceptible MIC values in SM-resistant Mtb.

    Graphical abstract

    Papers of special note have been highlighted as: • of interest

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