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Phenotypic detection of quorum sensing inhibition in Pseudomonas aeruginosa pyoverdine and swarming by volatile organic products

    Román Y Ramírez-Rueda

    *Author for correspondence:

    E-mail Address: royer94@gmail.com

    Department of Plant Biology, PPG BTPB & PPG BV, Institute of Biology, University of Campinas - UNICAMP, Postal Box 6109, Campinas – SP 13083-970, Brazil

    Faculty of Health Sciences. Pedagogical & Technological University of Colombia, Postal Box 0387437173 Tunja, Calle 24 N° 5-63, Colombia

    &
    Marcos J Salvador

    Department of Plant Biology, PPG BTPB & PPG BV, Institute of Biology, University of Campinas - UNICAMP, Postal Box 6109, Campinas – SP 13083-970, Brazil

    Published Online:https://doi.org/10.2217/fmb-2020-0033

    Aim: To determine phenotypically the anti quorum-sensing (QS) activity of 30 volatile organic products (VOPs) through the inhibition of swarming motility and pyoverdine production in Pseudomonas aeruginosa. Materials & methods: Twenty-four essential oils and six small volatile organic compounds randomly selected were screened for their anti-QS activity by violacein inhibition on Chromobacterium violaceum. The VOPs with positive results were subsequently evaluated for swarming motility and pyoverdine production on P. aeruginosa determining the colony diameter and fluorescence under UV light, respectively. Results: Fifty percent of VOPs tested showed strong violacein inhibition, 40% presented anti-swarming activity and 33% inhibited pyoverdine production. Conclusion: Our data demonstrate that VOPs have a great potential to inhibit virulence factors mediated by QS in P. aeruginosa

    Papers of special note have been highlighted as: • of interest; •• of considerable interest

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