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Bioactive molecule optimized for biofilm reduction related to childhood caries

    Gabriela F Bombarda

    Piracicaba Dental School, Department of Physiological Sciences, University of Campinas, Piracicaba, SP 13414 903, Brazil

    ,
    Pedro L Rosalen

    Piracicaba Dental School, Department of Physiological Sciences, University of Campinas, Piracicaba, SP 13414 903, Brazil

    ,
    Eder R Paganini

    Department of Chemistry & Environmental Sciences, University Júlio de MesquitaFilho, São Jose do Rio Preto, Brazil

    ,
    Mayara AR Garcia

    Department of Chemistry & Environmental Sciences, University Júlio de MesquitaFilho, São Jose do Rio Preto, Brazil

    ,
    Diego R Silva

    Piracicaba Dental School, Department of Physiological Sciences, University of Campinas, Piracicaba, SP 13414 903, Brazil

    ,
    Josy G Lazarini

    Piracicaba Dental School, Department of Physiological Sciences, University of Campinas, Piracicaba, SP 13414 903, Brazil

    ,
    Irlan A Freires

    Piracicaba Dental School, Department of Physiological Sciences, University of Campinas, Piracicaba, SP 13414 903, Brazil

    ,
    Luís O Regasini

    Department of Chemistry & Environmental Sciences, University Júlio de MesquitaFilho, São Jose do Rio Preto, Brazil

    &
    Janaina CO Sardi

    *Author for correspondence: Fax: +55 1921 065 308;

    E-mail Address: janasardi@gmail.com

    Piracicaba Dental School, Department of Physiological Sciences, University of Campinas, Piracicaba, SP 13414 903, Brazil

    Published Online:https://doi.org/10.2217/fmb-2019-0144

    Aim: To evaluate antimicrobial activity of a new nitrochalcone (NC-E08) against Candida albicans and Streptococcus mutans, and its toxicity. Materials & methods: Minimum inhibitory concentration (MIC) and minimum bactericidal concentration/minimum fungicidal concentration (MFC) were determined against C. albicans and S. mutans, as well as antibiofilm potential and toxicity (human gingival fibroblast and Galleria mellonella). Infection and treatment were performed in G. mellonella. Results & conclusion: NC-E08 showed antimicrobial activity in C. albicans (MIC: 0.054 mM) and S. mutans (MIC: 0.013 mM); 10xMIC treatment reduced 4.0 log10 biofilms for both strains and there was a reduction in survival of mixed biofilms of C. albicans and S. mutans (6.0 and 4.0 log10, respectively). NC-E08 showed no cytotoxicity in human gingival fibroblast cells and G. mellonella. NC-E08 after larval infection protected them 90% (p < 0.05). Thus, is a promising one for the prevention and treatment of S. mutans and C. albicans infections.

    Papers of special note have been highlighted as: • of interest; •• of considerable interest

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