We use cookies to improve your experience. By continuing to browse this site, you accept our cookie policy.×
Skip main navigation
Open Drawer MenuClose Drawer Menu
Aging Health
Bioelectronics in Medicine
Biomarkers in Medicine
Breast Cancer Management
CNS Oncology
Colorectal Cancer
Concussion
Epigenomics
Future Cardiology
Future Medicine AI
Future Microbiology
Future Neurology
Future Oncology
Future Rare Diseases
Future Virology
Hepatic Oncology
HIV Therapy
Immunotherapy
International Journal of Endocrine Oncology
International Journal of Hematologic Oncology
Journal of 3D Printing in Medicine
Lung Cancer Management
Melanoma Management
Nanomedicine
Neurodegenerative Disease Management
Pain Management
Pediatric Health
Personalized Medicine
Pharmacogenomics
Regenerative Medicine
Short Communication

A blaVIM-1 positive Aeromonas hydrophila strain in a near-drowning patient: evidence for interspecies plasmid transfer within the patient

    Thijs Bosch

    *Author for correspondence: Tel.: +31 (0)30 274 2105;

    E-mail Address: Thijs.bosch@rivm.nl

    Centre for Infectious Diseases Control, National Institute for Public Health & the Environment (RIVM), Bilthoven, The Netherlands

    Search for more papers by this author

    ,
    Rogier Schade

    Amsterdam UMC, Vrije Universiteit Amsterdam, Department of Medical Microbiology & Infection Control, Amsterdam, The Netherlands

    Search for more papers by this author

    ,
    Fabian Landman

    Centre for Infectious Diseases Control, National Institute for Public Health & the Environment (RIVM), Bilthoven, The Netherlands

    Search for more papers by this author

    ,
    Leo Schouls

    Centre for Infectious Diseases Control, National Institute for Public Health & the Environment (RIVM), Bilthoven, The Netherlands

    Search for more papers by this author

    &
    Karin van Dijk

    Amsterdam UMC, Vrije Universiteit Amsterdam, Department of Medical Microbiology & Infection Control, Amsterdam, The Netherlands

    Search for more papers by this author

    Published Online:18 Oct 2019https://doi.org/10.2217/fmb-2019-0091

    Abstract

    Aim: To show that a strain of Aeromonas hydrophila became resistant to carbapenems by interspecies transfer of a plasmid using long-read sequencing. Material & methods: Whole genome sequencing of the four isolates was done using Illumina Hiseq, while the plasmid was reconstructed using the MinION sequencer. The resistome was identified with ResFinder. Results: Whole genome sequencing and long-read sequencing showed that all isolates carried a blaVIM-1 gene located on a 165 kb incA/C plasmid. ResFinder confirmed that the resistome of the plasmid, comprising 13 resistance genes, was identical within all isolates. Discussion: Long-read sequencing using the MinION successfully reconstructed a plasmid that was identical in all isolates, providing evidence for horizontal gene transfer of this blaVIM-1 gene carrying plasmid within the patient.

    Papers of special note have been highlighted as: •• of considerable interest

    References

    • 1. Goncalves Pessoa RB, de Oliveira WF, Marques DSC et al. The genus Aeromonas: a general approach. Microb. Pathog. 130, 81–94 (2019).
      MedlineGoogle Scholar
    • 2. Zhou Y, Yu L, Nan Z et al. Taxonomy, virulence genes and antimicrobial resistance of Aeromonas isolated from extra-intestinal and intestinal infections. BMC Infect. Dis. 19(1), 158 (2019).
      MedlineGoogle Scholar
    • 3. Rasmussen-Ivey CR, Figueras MJ, McGarey D, Liles MR. Virulence factors of Aeromonas hydrophila: in the wake of reclassification. Front.Microbiol. 7, 1337 (2016).
      MedlineGoogle Scholar
    • 4. Piotrowska M, Popowska M. Insight into the mobilome of Aeromonas strains. Front. Microbiol. 6, 494 (2015).
      MedlineGoogle Scholar
    • 5. Walsh TR, Payne DJ, MacGowan AP, Bennett PM. A clinical isolate of Aeromonas sobria with three chromosomally mediated inducible beta-lactamases: a cephalosporinase, a penicillinase and a third enzyme, displaying carbapenemase activity. J. Antimicrob. Chemother. 35(2), 271–279 (1995).
      Medline CASGoogle Scholar
    • 6. Ko WC, Wu HM, Chang TC, Yan JJ, Wu JJ. Inducible beta-lactam resistance in Aeromonas hydrophila: therapeutic challenge for antimicrobial therapy. J. Clin. Microbiol. 36(11), 3188–3192 (1998).
      Medline CASGoogle Scholar
    • 7. Balsalobre LC, Dropa M, Lincopan N et al. Detection of metallo-beta-lactamases-encoding genes in environmental isolates of Aeromonas hydrophila and Aeromonas jandaei. Lett. Appl. Microbiol. 49(1), 142–145 (2009).
      Medline CASGoogle Scholar
    • 8. Libisch B, Giske CG, Kovacs B, Toth TG, Fuzi M. Identification of the first VIM metallo-beta-lactamase-producing multiresistant Aeromonas hydrophila strain. J. Clin. Microbiol. 46(5), 1878–1880 (2008). •• Describe the blaVIM producing A. hydrophila strain.
      Medline CASGoogle Scholar
    • 9. Neuwirth C, Siebor E, Robin F, Bonnet R. First occurrence of an IMP metallo-beta-lactamase in Aeromonas caviae: IMP-19 in an isolate from France. Antimicrob. Agents Chemother. 51(12), 4486–4488 (2007).
      Medline CASGoogle Scholar
    • 10. Carattoli A. Plasmids and the spread of resistance. Int. J. Med. Microbiol. 303(6-7), 298–304 (2013).
      Medline CASGoogle Scholar
    • 11. Conlan S, Thomas PJ, Deming C et al. Single-molecule sequencing to track plasmid diversity of hospital-associated carbapenemase-producing Enterobacteriaceae. Sci. Transl. Med. 6(254), 254ra126 (2014).
      MedlineGoogle Scholar
    • 12. van der Zwaluw K, de Haan A, Pluister GN et al. The carbapenem inactivation method (CIM), a simple and low-cost alternative for the Carba NP test to assess phenotypic carbapenemase activity in Gram-negative rods. PLoS ONE 10(3), e0123690 (2015).
      MedlineGoogle Scholar
    • 13. Carattoli A, Zankari E, Garcia-Fernandez A et al. In silico detection and typing of plasmids using PlasmidFinder and plasmid multilocus sequence typing. Antimicrob. Agents Chemother. 58(7), 3895–3903 (2014).
      MedlineGoogle Scholar
    • 14. Zankari E, Hasman H, Cosentino S et al. Identification of acquired antimicrobial resistance genes. J. Antimicrob. Chemother. 67(11), 2640–2644 (2012). •• Providesthe web server for identifying acquired antimicrobial resistance genes applied in this study.
      Medline CASGoogle Scholar
    • 15. Koren S, Walenz BP, Berlin K et al. Canu: scalable and accurate long-read assembly via adaptive k-mer weighting and repeat separation. Genome Res. 27(5), 722–736 (2017).
      Medline CASGoogle Scholar
    • 16. Chen PL, Ko WC, Wu CJ. Complexity of beta-lactamases among clinical Aeromonas isolates and its clinical implications. J. Microbiol. Immunol.Infect. 45(6), 398–403 (2012).
      MedlineGoogle Scholar
    • 17. Adler A, Assous MV, Paikin S et al. Emergence of VIM-producing Aeromonas caviae in Israeli hospitals. J. Antimicrob. Chemother. 69(5), 1211–1214 (2014). •• Describes the emergence of human cases with blaVIM producing Aeromonas strains.
      Medline CASGoogle Scholar
    • 18. Antonelli A, D’Andrea MM, Montagnani C et al. Newborn bacteraemia caused by an Aeromonas caviae producing the VIM-1 and SHV-12 beta-lactamases, encoded by a transferable plasmid. J. Antimicrob. Chemother. 71(1), 272–274 (2016).
      MedlineGoogle Scholar
    • 19. de Lannoy C, de Ridder D, Risse J. The long reads ahead: de novo genome assembly using the MinION. F1000Res 6, 1083 (2017). •• Provides a very detailed and up-to-date review on long-read sequencing using the nanopore technology.
      MedlineGoogle Scholar
    • 20. George S, Pankhurst L, Hubbard A et al. Resolving plasmid structures in Enterobacteriaceae using the MinION nanopore sequencer: assessment of MinION and MinION/Illumina hybrid data assembly approaches. Microb. Genom. 3(8), e000118 (2017).
      MedlineGoogle Scholar
    • 21. Wick RR, Judd LM, Gorrie CL, Holt KE. Unicycler: resolving bacterial genome assemblies from short and long sequencing reads. PLoS Comput.Biol. 13(6), e1005595 (2017).
      MedlineGoogle Scholar