Research Article

Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China

‡Authors contributed equally

Key Laboratory of Cell Proliferation & Regulation Biology of Ministry of Education, Institute of Cell Biology, College of Life Sciences, Beijing Normal University, Beijing 100875, China

Department of Immunology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China

‡Authors contributed equally

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Yu Wang

Department of Immunology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China

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Yan Li

Key Laboratory of Cell Proliferation & Regulation Biology of Ministry of Education, Institute of Cell Biology, College of Life Sciences, Beijing Normal University, Beijing 100875, China

Department of Immunology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China

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Lin Dong

Key Laboratory of Cell Proliferation & Regulation Biology of Ministry of Education, Institute of Cell Biology, College of Life Sciences, Beijing Normal University, Beijing 100875, China

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Li Li

Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China

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Junhao Zhu

Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China

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QiangQiang Zhang

Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China

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Guangwei Liu

Key Laboratory of Cell Proliferation & Regulation Biology of Ministry of Education, Institute of Cell Biology, College of Life Sciences, Beijing Normal University, Beijing 100875, China

Department of Immunology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China

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Jinhua Xu

Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China

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Min Zhu

*Author for correspondence:

E-mail Address: juneminmyco@126.com

Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China

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Published Online:4 Jun 2019https://doi.org/10.2217/fmb-2019-0043

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Abstract

Aim:Aspergillus fumigatus is one of the most common opportunistic fungi that can cause invasive infection. To profile the kinetic variation of immune cells and cytokines after exposure to A. fumigatus thoroughly, we established a pulmonary A. fumigatus infection model in temporarily immunosuppressed mice. Materials & methods: Systematic and kinetic studies of different immune cells and cytokines were performed. Results: We observed that the granulocytes and macrophages recruited to the site of infection played an important role in the infectious phase. There was a significant increase in the cytokines IFN-γ, IL-6, TNF-α as well as the chemokines CXCL1, MIP-1α, MIP-2 and CCL5 after infection. IL-10 was found to participate in balancing the anti-inflammatory response in the recovery phases. The immune response mediated by T cells was mainly presented by the Th1-type on day 7 after exposure with a high proportion of IFN-γ⁺ CD4⁺ T cells and CD4⁺CD44^highCD62L^low effector T cells. Conclusion: These kinetic parameters of the immune response might provide diagnostic clues for A. fumigatus infection.

Keywords:

Papers of special note have been highlighted as: • of interest; •• of considerable interest

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Vol. 14, No. 8

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History

Received 11 February 2019

Accepted 9 April 2019

Published online 4 June 2019

Published in print May 2019

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Keywords

Supplementary data

To view the supplementary data that accompany this paper please visit the journal website at: www.futuremedicine.com/doi/full/10.2217/fmb-2019-0043

Author contributions

H Su wrote the manuscript; H Su, C Li, Y Wang, Y Li and L Dong designed and conducted the experiment with cells and mice and analyzed data; L Li, J Zhu and Q Zhang assisted with analysis and interpretation of experiments and results. M Zhu, G Liu and J Xu developed the concept, designed and conducted the experiments. H Su and C Li contributed equally to this work.

Financial & competing interests disclosure

This study was funded by the ‘973 Program’ of Ministry of Science and Technology of the People’s Republic of China (2013CB536005). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

This study was carried out in accordance with the recommendations of Animal Ethics Committee of Fudan University. The protocol was approved by the Animal Ethics Committee of Fudan University, Shanghai, China.

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Kinetic host defense of the mice infected with Aspergillus Fumigatus

Abstract

References