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Diversity of Acinetobacter species isolated from different environments in Lebanon: a nationwide study

Ahmad Al Atrouni

Laboratoire Microbiologie Santé et Environnement (LMSE), Ecole Doctorale des Sciences et de Technologie et Faculté de Santé Publique, Université Libanaise, Tripoli, Liban

ATOMycA, Inserm Atip-Avenir Team, CRCNA, Inserm U892, 6299 CNRS, University of Angers, France

,
Monzer Hamze

Laboratoire Microbiologie Santé et Environnement (LMSE), Ecole Doctorale des Sciences et de Technologie et Faculté de Santé Publique, Université Libanaise, Tripoli, Liban

,
Rayane Rafei

Laboratoire Microbiologie Santé et Environnement (LMSE), Ecole Doctorale des Sciences et de Technologie et Faculté de Santé Publique, Université Libanaise, Tripoli, Liban

,
Matthieu Eveillard

ATOMycA, Inserm Atip-Avenir Team, CRCNA, Inserm U892, 6299 CNRS, University of Angers, France

Laboratoire de Bactériologie, Institut de Biologie en Santé – Centre Hospitalier Universitaire, Angers, France

,
Marie-Laure Joly-Guillou

ATOMycA, Inserm Atip-Avenir Team, CRCNA, Inserm U892, 6299 CNRS, University of Angers, France

Laboratoire de Bactériologie, Institut de Biologie en Santé – Centre Hospitalier Universitaire, Angers, France

&
Marie Kempf

*Author for correspondence:

E-mail Address: makempf@chu-angers.fr

ATOMycA, Inserm Atip-Avenir Team, CRCNA, Inserm U892, 6299 CNRS, University of Angers, France

Laboratoire de Bactériologie, Institut de Biologie en Santé – Centre Hospitalier Universitaire, Angers, France

Published Online:https://doi.org/10.2217/fmb-2016-0082

Aim: To investigate the extrahospital reservoirs of Acinetobacter spp. in Lebanon. Materials & methods: Two thousand three hundred and sixty-one samples from different ecological niches were analyzed by culture methods. Species identification was confirmed by rpoB-gene sequencing. Multilocus sequence typing was used to characterize the Acinetobacter baumannii clones. Results & conclusion:Acinetobacter spp. were detected in 14% of environmental samples and 8% of food samples. Furthermore, 9% of animals and 3.4% of humans were colonized. Non-baumannii Acinetobacter were the most common species isolated and newly susceptible A. baumannii clones were detected. Interestingly, 21 isolates were not identified at the species level and were considered as putative novel species. To our knowledge, this is the largest epidemiological study investigating the epidemiology of Acinetobacter spp. outside hospitals.

Papers of special note have been highlighted as: • of interest; •• of considerable interest

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