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Research Article

Regulation of microRNAs in coronary atherosclerotic plaque

    Öcal Berkan

    Department of Cardiovascular Surgery, Çiğli Training & Research Hospital, İzmir 35620, Turkey

    ,
    Serdal Arslan

    Department of Medical Biology, Faculty of Medicine, Sivas Cumhuriyet University, Sivas 58140, Turkey

    ,
    Torkia Lalem

    Cardiovascular Research Unit, Luxembourg Institute of Health, Strassen L-1445, Luxembourg

    ,
    Lu Zhang

    Cardiovascular Research Unit, Luxembourg Institute of Health, Strassen L-1445, Luxembourg

    ,
    Nil Özbilum Şahin

    Department of Molecular Biology & Genetics, Faculty of Science, Sivas Cumhuriyet University, Sivas 58140, Turkey

    ,
    Eylem Itır Aydemir

    Department of Statistics, Faculty of Science, Sivas Cumhuriyet University, Sivas 58140, Turkey

    ,
    Özge Korkmaz

    Department of Cardiovascular Surgery, Koru Hospital, Yüksek İhtisas University, Ankara 06510, Turkey

    ,
    Hatice Reyhan Eğilmez

    Department of Pathology Faculty of Medicine, Sivas Cumhuriyet University, Sivas 58140, Turkey

    ,
    Nilgün Çekin

    Department of Medical Biology, Faculty of Medicine, Sivas Cumhuriyet University, Sivas 58140, Turkey

    &
    Yvan Devaux

    *Author for correspondence: Tel.: +352 2697 0300; Fax: +352 2697 0719;

    E-mail Address: yvan.devaux@lih.lu

    Cardiovascular Research Unit, Luxembourg Institute of Health, Strassen L-1445, Luxembourg

    Published Online:https://doi.org/10.2217/epi-2019-0036

    Aim: Identification of microRNAs (miRNAs) associated with atherosclerosis may unravel novel therapeutic targets and biomarkers. We studied miRNAs differentially expressed between coronary atherosclerotic plaques (CAP) and healthy arteries. Materials & methods: Paired CAP and internal mammary arteries (IMA) were collected from 14 coronary artery disease patients. The miRNA profiles between diseased (CAP) and healthy (IMA) tissues were compared using microarrays and quantitative PCR. Results: Thirty-one miRNAs were differentially expressed between CAP and IMA. Among these, miR-486-5p showed a high level of regulation (12-fold), had predicted interactions with atherosclerosis-associated genes and correlated with triglyceride levels and arterial stenosis. Regulation of miR-486-5p was validated by PCR (p = 0.004). Conclusion: The miRNAs are regulated in the atherosclerotic plaque. We highlight miR-486-5p whose role in atherosclerosis requires further investigation.

    Papers of special note have been highlighted as: • of interest; •• of considerable interest

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