Research Article

Shear stress induces osteogenic differentiation of human mesenchymal stem cells

Department of Physiology & Biophysics, University of Illinois at Chicago, 835 S. Wolcott Avenue, Rm. E-208, MC901, Chicago, IL 60612, USA

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Susan M McCormick

Department of Bioengineering, University of Illinois at Chicago, 851 South Morgan Street, MC 063, Chicago, IL 60607, USA

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Jeremy J Mao

Tissue Engineering & Regenerative Medicine Laboratory, Columbia University Medical Center, 630 W. 168 Sreet, PH7 East, CDM, New York, NY 10032, USA

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Gwendolen C Reilly

† Author for correspondence

Kroto Research Institute, Department of Materials Science & Engineering, University of Sheffield, Broad Lane, Sheffield, S3 7HQ, UK.

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Email the corresponding author at g.reilly@sheffield.ac.uk

Published Online:27 Sep 2010https://doi.org/10.2217/rme.10.60

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Abstract

Aim: To determine whether fluid flow-induced shear stress affects the differentiation of bone marrow-derived human mesenchymal stem cells (hMSCs) into osteogenic cells. Materials & methods: hMSCs cultured with or without osteogenic differentiation medium were exposed to fluid flow-induced shear stress and analyzed for alkaline phosphatase activity and expression of osteogenic genes. Results: Immediately following shear stress, alkaline phosphatase activity in osteogenic medium was significantly increased. At days 4 and 8 of culture the mRNA expression of bone morphogenetic protein-2 and osteopontin was significantly higher in hMSCs subjected to shear stress than those cultured in static conditions. However, hMSCs cultured in osteogenic differentiation medium were less responsive in gene expression of alkaline phosphatase and bone morphogenetic protein-2. Conclusion: These data demonstrate that shear stress stimulates hMSCs towards an osteoblastic phenotype in the absence of chemical induction, suggesting that certain mechanical stresses may serve as an alternative to chemical stimulation of stem cell differentiation.

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Published online 27 September 2010

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Financial & competing interests disclosure

This work was supported by the NIH, grant number: NIH5RC2DE020767. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.

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