Preliminary Communication

Modification of the inflammatory profile of mesenchymal stem cells using different culture conditions

*Author for correspondence:

E-mail Address: alison.annette.smith@gmail.com

Department of Surgery, Tulane University, 1430, Tulane Ave New Orleans, LA 70112, USA

Department of Surgery, Lousiana State University, Health Sciences Center, 2021, Perdido Street New Orleans, LA 70112, USA

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Charles F Bellows

https://orcid.org/0000-0002-2243-0517

Department of Surgery, Tulane University, 1430, Tulane Ave New Orleans, LA 70112, USA

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Published Online:15 Feb 2024https://doi.org/10.2217/rme-2023-0162

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Abstract

Aim: Mesenchymal stem cells (MSCs) are pluripotent cells with significant therapeutic potential. The objective of this study was to examine the inflammatory profile of MSCs cultured under different conditions. Methods: MSCs were cultured by three strategies: seeding on an extracellular matrix (ECM), spheroids in static culture and spheroids in a bioreactor. Paracrine factors and CD206, a marker of M2 macrophage phenotype, were measured. Results: MSCs grown as spheroids in a bioreactor produced more IL-6 and IL-8 (p < 0.05). Supernatant collected from spheroids under both culture conditions increased the M2 macrophage phenotype almost twofold. Conclusion: Results indicate that the inflammatory profile of the supernatant collected from MSCs can be modified through culture conditions which has impacts for the future of regenerative medicine.

Graphical abstract

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Papers of special note have been highlighted as: • of interest; •• of considerable interest

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Vol. 19, No. 2

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History

Received 10 August 2023

Accepted 5 February 2024

Published online 15 February 2024

Published in print February 2024

Information

Keywords

Author contributions

AA Smith: conceptualization, formal analysis, methodology, writing – original draft. CF Bellows: conceptualization, supervision, writing – review and editing.

Acknowledgments

The authors would like to thank D Phinney for providing the mesenchymal stem cells and technical assistance. The graphical abstract was created with BioRender.com. This research was presented as a poster at the 44th annual 2021 Shock Society Virtual Meeting.

Financial disclosure

The authors have no financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties.

Competing interests disclosure

AA Smith is a consultant for Aroa Biosurgery and on the advisory board for Prytime Medical. The authors have no other competing interests or relevant affiliations with any organization or entity with the subject matter or materials discussed in the manuscript apart from those disclosed.

Writing disclosure

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations.

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