Research Article

Purified exosome product enhances chondrocyte survival and regeneration by modulating inflammation and promoting chondrogenesis

Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN 55901, USA

Department of Orthopaedics, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, China

‡Authors contributed equally

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Zeling Long‡

Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN 55901, USA

‡Authors contributed equally

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Rodolfo E De la Vega

Musculoskeletal Gene Therapy Research Laboratory, Rehabilitation Medicine Research Center, Mayo Clinic, Rochester, MN 55901, USA

Department cBITE, MERLN Institute, Maastricht University, Maastricht, 6221, The Netherlands

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Atta Behfar

Department of Cardiovascular Diseases, Van Cleve Cardiac Regenerative Medicine Program, Center for Regenerative Medicine, Mayo Clinic, Rochester, MN 55901, USA

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Steven L Moran

Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN 55901, USA

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Christopher Evans

Musculoskeletal Gene Therapy Research Laboratory, Rehabilitation Medicine Research Center, Mayo Clinic, Rochester, MN 55901, USA

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Chunfeng Zhao

*Author for correspondence: Tel.: +1 507 538 1296;

E-mail Address: zhaoc@mayo.edu

Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN 55901, USA

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Published Online:18 Oct 2022https://doi.org/10.2217/rme-2022-0132

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Abstract

Aim: This study was to detect the effects of purified exosome product (PEP) on C28/I2 cells and chondrocytes derived from osteoarthritis patients. Materials & methods: Cell viability and apoptosis assays were used to detect the effect of PEP on cells. qRT-PCR and cell fluorescence assays were used to investigate the potential mechanism of PEP on cell chondrogenesis. Results: PEP was internalized by cells at a fast rate and enhanced cellular proliferation and migration while attenuating apoptosis. These findings reflect the fact that PEP can increase the expression of PCNA and reduce the expression of CASP3/7/9 and BAX. Conclusion: This study suggests an innovative strategy for chondrogenesis that could be applied to osteoarthritis repair in the future.

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Purified exosome product acts as an efficient chondroprotective and regenerative agent by enhancing proliferation, attenuating apoptosis and modulating inflammation.

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Papers of special note have been highlighted as: • of interest; •• of considerable interest

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Vol. 18, No. 1

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History

Received 29 July 2022

Accepted 28 September 2022

Published online 18 October 2022

Published in print January 2023

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Keywords

Author contributions

G Shi designed the study, performed the experiments, acquired the data, analyzed and interpreted data and drafted the manuscript. Z Long, R de la Vega, A Behfar, C Evans and C Zhao contributed to study design, data interpretation, manuscript editing and finalizing. Z Long also helped perform the experiments and analyzed the data. All authors have read and approved the final submitted manuscript.

Financial & competing interests disclosure

This study was supported by grants from the Orthopedic Research Review Committee of Mayo Clinic (94064024), NIH NIAMS AR57745 and the Mayo Clinic Kelly Fellow Award. This study also received technical support from Ramona. The authors G Shi and Z Long are funded by the China Scholarship Council. G Shi is also funded by the postgraduate Innovation Fund of ‘13th Five-Year comprehensive investment’ of Tianjin Medical University (YJSCX201903) and Tianjin Research Innovation Project for Postgraduate Students (2019YJSB109). A Behfar is a co-founder of Rion, LLC. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.

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