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Regenerative Medicine

Combined use of calcium phosphate cement, mesenchymal stem cells and platelet-rich plasma for bone regeneration in critical-size defect of the femoral condyle in mini-pigs

    Guangjun Li‡

    *Author for correspondence: Tel.: +86 188 6821 5006;

    E-mail Address: joseph06113033@126.com

    Department of Orthopedic, Deqing People’s Hospital, Deqing, Zhejiang 313200, PR China

    ‡Authors contributed equally

    Search for more papers by this author

    ,
    Wen Shen‡

    Department of Radiology, Deqing People’s Hospital, Deqing, Zhejiang 313200, PR China

    ‡Authors contributed equally

    Search for more papers by this author

    ,
    Xing Tang

    Department of Orthopedic, Deqing People’s Hospital, Deqing, Zhejiang 313200, PR China

    ,
    Guowei Mo

    Department of Orthopedic, Deqing People’s Hospital, Deqing, Zhejiang 313200, PR China

    ,
    Liqin Yao

    Department of Orthopedic, Deqing People’s Hospital, Deqing, Zhejiang 313200, PR China

    &
    Jixing Wang

    **Author for correspondence:

    E-mail Address: wjxwsy@sina.cn

    Department of Spinal Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, PR China

    Published Online:https://doi.org/10.2217/rme-2020-0099

    Aim: To investigate the outcome of autologous bone marrow mesenchymal stem cells (BMMSCs) and platelet-rich plasma in combination with calcium phosphate cement (CPC) scaffold to reconstruct femoral critical bone defects in mini-pigs. Materials & methods: Scanning electron microscopy, micro-computed tomography evaluation and quantitative histological assessment were used. Results & conclusion: BMMSCs were attached to the CPC scaffold after 7 days of culture and decreased the residual CPC material in each group at 12 weeks compared with 6 weeks. The newly formed bone area was higher in the CPC+SC+P group than in the CPC group at each time point (all p < 0.05). The strategy of CPC combined with BMMSCs and platelet-rich plasma might be an effective method to repair bone defects.

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