Research Article

Adipose tissue-derived regenerative cells improve implantation of fertilized eggs in thin endometrium

Fusanori Yotsumoto

*Author for correspondence:

E-mail Address: yotsumoto@cis.fukuoka-u.ac.jp

https://orcid.org/0000-0001-9411-476X

Department of Obstetrics & Gynecology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan

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Hideki Iwaguro

Department of Obstetrics & Gynecology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan

SOBAJIMA Clinic, Osaka, Japan

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Yusuke Harada

SOBAJIMA Clinic, Osaka, Japan

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Satoshi Sobajima

SOBAJIMA Clinic, Osaka, Japan

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Takako Suwabe

Department of Obstetrics & Gynecology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan

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Shingo Miyamoto

**Author for correspondence:

E-mail Address: smiya@cis.fukuoka-u.ac.jp

Department of Obstetrics & Gynecology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan

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Published Online:23 Jul 2020https://doi.org/10.2217/rme-2020-0037

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Abstract

Aim: Embryo implantation and subsequent pregnancy depends on endometrial thickness. To investigate potential fertility strategies for women with thin endometrium, we explored the efficacy of adipose tissue-derived regenerative cells (ADRCs) on thin endometrium and embryo implantation in a mouse model. Materials & methods: ADRCs isolated from mouse subcutaneous fat were characterized by flow cytometry. Endometrium thickness, endometrial fibrosis, embryo implantation and angiogenesis factors were evaluated in uterine cavities of ethanol-induced thin endometrium mice with ADRC transplantation. Results: ADRCs included adipose-derived stem cells and some blood vessel component cells. ADRCs improved endometrial thickness, endometrial fibrosis and embryo implantation and augmented vascular endothelial growth factor expression in the mouse uterine. Conclusion: ADRCs may be a useful therapeutic strategy to improve fertility of women with thin endometrium.

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Papers of special note have been highlighted as: • of interest; •• of considerable interest

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Vol. 15, No. 7

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Received 21 March 2020

Accepted 3 July 2020

Published online 23 July 2020

Published in print July 2020

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Supplementary data

To view the supplementary data that accompany this paper please visit the journal website at: www.futuremedicine.com/doi/suppl/10.2217/rme-2020-0037

Author contributions

Conception or design of the work was performed by F Yotsumoto and S Miyamoto. Acquisition of data was performed by F Yotsumoto, T Suwabe and Y Harada. Analysis and interpretation of data were performed by F Yotsumoto, H Iwaguro and S Miyamoto. Drafting of the article was performed by F Yotsumoto and S Miyamoto. Critical revision of the article for important intellectual content was performed by F Yotsumoto, S Sobajima and S Miyamoto. Final approval of the version to be published was given by F Yotsumoto and S Miyamoto. All authors have read and approved the final version of the manuscript.

Acknowledgments

We thank GW Wolf from Edanz Group (https://en-author-services.edanzgroup.com/) for editing a draft of this manuscript and K Nakajima (Joint Laboratory for Frontier Medical Science, Faculty of Medicine, Fukuoka University, Fukuoka, Japan) for technical assistance.

Financial & competing interests disclosure

This work was supported in part by JSPS KAKENHI Grant Number 18K09242 and a Grant-in-Aid from the Kakihara Science and Technology Foundation (Fukuoka, Japan) to S Miyamoto. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations.

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