Preliminary Communication

Repair of lumbar vertebral bone defects by bone particles combined with hUC-MSCs in weaned rabbit

Reproductive & Genetic Center, National Research Institute for Family Planning, Beijing 100081, PR China

†Authors contributed equally

Key Laboratory of Molecular Developmental Biology, Institute of Genetics & Developmental Biology, Chinese Academy of Sciences, Beijing 100080, PR China

†Authors contributed equally

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Yanyun Yin^†

Key Laboratory of Molecular Developmental Biology, Institute of Genetics & Developmental Biology, Chinese Academy of Sciences, Beijing 100080, PR China

†Authors contributed equally

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Bing Chen

Key Laboratory of Molecular Developmental Biology, Institute of Genetics & Developmental Biology, Chinese Academy of Sciences, Beijing 100080, PR China

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Yannan Zhao

Key Laboratory of Molecular Developmental Biology, Institute of Genetics & Developmental Biology, Chinese Academy of Sciences, Beijing 100080, PR China

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Zhifeng Xiao

Key Laboratory of Molecular Developmental Biology, Institute of Genetics & Developmental Biology, Chinese Academy of Sciences, Beijing 100080, PR China

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Bin Yang

Key Laboratory of Molecular Developmental Biology, Institute of Genetics & Developmental Biology, Chinese Academy of Sciences, Beijing 100080, PR China

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Qingyuan Meng

Key Laboratory of Molecular Developmental Biology, Institute of Genetics & Developmental Biology, Chinese Academy of Sciences, Beijing 100080, PR China

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Yongxiang Fang

State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Lanzhou Veterinary Research Institute, CAAS, Lanzhou 730046, PR China

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Qinghan Liang^‡

Department of Gynaecology, Beijing haidian maternal & child health hospital, Beijing 100089, PR China

†Authors contributed equally

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Ling Zhou^‡

Department of Gynaecology & Obstetrics, Strategic Support Force Medical Centre of PLA, Beijing 100101, PR China

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Xu Ma^‡

Reproductive & Genetic Center, National Research Institute for Family Planning, Beijing 100081, PR China

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Jianwu Dai

*Author for correspondence:

E-mail Address: xda_da@genentics.ac.cn

Key Laboratory of Molecular Developmental Biology, Institute of Genetics & Developmental Biology, Chinese Academy of Sciences, Beijing 100080, PR China

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Published Online:26 Sep 2019https://doi.org/10.2217/rme-2018-0134

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Abstract

Aim: The major symptom of many closed spinal dysraphism patients is that the laminas or arches of vertebra are not fused well. To date, the bone repair of spina bifida for young children is a significant challenge in clinical practice. Materials & methods: Bovine bone collagen particle (BBCP) scaffolds combined with human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) were implanted in the defect area. X-ray analysis was performed after 3 months. Tissues were harvested for gross observation, and histological and immunohistochemical staining. Results: The BBCP supported hUC-MSCs adhesion and growth. Implanted BBCP combined with hUC-MSCs also promoted bone regeneration in the vertebral lamina and arch defect area. Conclusion: This method represents a new strategy for vertebral lamina and arch reconstruction in children.

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Vol. 14, No. 10

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History

Received 16 October 2018

Accepted 10 September 2019

Published online 26 September 2019

Published in print October 2019

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Financial & competing interests disclosure

This work was supported by National Research Institute for National Key R&D Program of China (2016YFC1000806/2016YFC1000800), Family Planning (2016GJ Z06), the National Science Foundation of China (81601084) and ‘Strategic Priority Research Program of the Chinese Academy of Sciences’ (XDA16020100). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

Medical writing support was provided by Mitchell Arico from Liwen Bianji, Edanz Group China (www.liwenbianji.cn/ac) and was funded by the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA16020100).

Ethical conduct of research

The authors state that all animal procedures were performed in accordance with Chinese Ministry of Public Health Guide and US National Institute of Health (NIH) Guide for the care and use of laboratory animals and was approved by the Institutional Animal Care and Use Committee-Bioethics Committee of the Institute of genetics and developmental biology, Chinese academy of sciences.

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