Preliminary Communication

The role of nanofibrous structure in osteogenic differentiation of human mesenchymal stem cells with serial passage

Luong TH Nguyen

† Author for correspondence

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Email the corresponding author at hienluong@nus.edu.sg

Susan Liao

Nanyang Technological University, 637551 Singapore

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Seeram Ramakrishna

National University of Singapore, 117576 Singapore

Institute of Materials Research & Engineering, 117602 Singapore

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Casey K Chan

National University of Singapore, 117576 Singapore

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Published Online:26 Aug 2011https://doi.org/10.2217/nnm.11.26

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Abstract

Using scaffolds with autologous stem cells is a golden strategy for the treatment of bone defects. In this strategy, human mesenchymal stem cells (hMSCs) have often been isolated and expanded in vitro on a plastic surface to obtain a sufficient cell number before seeding on a suitable scaffold. Materials & Methods: Investigating the influence of serial passages (from passage two to passage eight) on the abilities of proliferation and osteogenic differentiation of hMSCs on 24-well tissue culture polystyrene plates and poly L-lactic acid electrospun nanofibrous scaffolds was performed to determine how prolonged culture affected these cellular abilities and how the nanofibrous scaffolds supported the osteogenic differentiation potential of hMSCs. Results & Conclusion: Serial passage caused adverse changes in hMSCs characteristics, which were indicated by the decline in both proliferation and osteogenic differentiation abilities. Interestingly, the poly L-lactic acid nanofibrous scaffolds showed a significant support in recovering the osteogenic abilities of hMSCs, which had been severely affected by prolonged culture.

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Papers of special note have been highlighted as: ▪ of interest ▪▪ of considerable interest

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Published online 26 August 2011

Published in print August 2011

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Financial & competing interests disclosure

This study was supported by NMRC/1151/2008, Singapore. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

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