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Research Article

Biogenic synthesis of gold nanoparticles using dual extract of tulsi–Vinca for breast cancer tumor regression in mice

    Ravi Pratap

    Department of Physics, Indian Institute of Technology (BHU), Varanasi, 221005, India

    ,
    Karishma Niveria‡

    Nanobiotech Lab Kirori Mal College University of Delhi, Delhi, 110007, India

    ‡Authors contributed equally

    Search for more papers by this author

    ,
    Saurabh Kumar Srivastava‡

    Department of Physics, Indian Institute of Technology (BHU), Varanasi, 221005, India

    ‡Authors contributed equally

    Search for more papers by this author

    ,
    Shilpi Chaudhary

    Department of Physics, Punjab Engineering College (Deemed to be University), Chandigarh, 160012, India

    ,
    Poonam Sharma

    Department of Dravyagun, Faculty of Ayurveda, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India

    ,
    Anita K Verma

    *Author for correspondence:

    E-mail Address: akverma@kmc.du.ac.in

    Nanobiotech Lab Kirori Mal College University of Delhi, Delhi, 110007, India

    Institution of Eminence Fellow, Delhi School of Public Health, Institution of Eminence, University of Delhi, Delhi, 110007, India

    &
    Avanish Singh Parmar

    **Author for correspondence:

    E-mail Address: asparmar.phy@itbhu.ac.in

    Department of Physics, Indian Institute of Technology (BHU), Varanasi, 221005, India

    Centre for Biomaterials and Tissue Engineering, Indian Institute of Technology (BHU), Varanasi, 221006, India

    Published Online:https://doi.org/10.2217/nnm-2023-0208

    Aim: This work aims to synthesize the gold nanoparticles (GNPs) using a dual extract of tulsi and Vinca (T+V-Gold) for breast cancer tumor regression. Methods: The GNPs were synthesized and characterized for their microscopic, spectroscopic and crystalline properties. Further, the GNPs were investigated for in vitro and in vivo studies for the treatment of the 4T1-induced triple-negative breast cancer murine model. Results: The GNPs for 4T1 tumor-challenged mice resulted in delayed tumor development and lower tumor burden, with T+V-Gold demonstrating the highest prevention of tumor spread. The antitumor effect of T+V-Gold is highly significant in the glutathione family antioxidants glutathione S-transferase and glutathione in tumor tissue samples. Conclusion: The bioefficacy and anticancer outcomes of T+V-Gold nanoformulation can be used as therapeutic agents and drug-delivery vehicles.

    Papers of special note have been highlighted as: • of interest; •• of considerable interest

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